Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid f...Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid fraction of the Chinese medicinal herb Huperzia serrata (Thunb.) Trev. Their structures and relative configurations were elucidated on the basis of spectroscopic analyses.展开更多
A full life-cycle toxicity test, combined with histology, on calanoid copepod Schmackeria poplesia was used to study the effect of bis（tributyltin） oxide （TBTO）. The results indicate no sex-specific differences in...A full life-cycle toxicity test, combined with histology, on calanoid copepod Schmackeria poplesia was used to study the effect of bis（tributyltin） oxide （TBTO）. The results indicate no sex-specific differences in TBTO toxicity. Long-term mortalities of the copepods exposed to concentrations higher than 20 ng TBTO L＂1 were significantly elevated compared with that of control, and larval development was inhibited when they were exposed to 40 and 60 ng TBTO L-1. The percentages of ovigerous females were reduced compared with the control （P〈0.01） after 24 days exposure to concentrations higher than 10 ng TBTO L-1. Histological examinations suggest that exposure to TBTO might block the posterior end of the diverticula and inhibits the production of egg sacs. A modified Euler-Lotka equation was used to calculate a population-level endpoint, the intrinsic rate of natural increase （rm）, from individual life-table endpoints, i.e. mortality rate, time of release of first brood, sex ratio, the fraction of ovigerous females among all females as well as the number of nauplii per ovigerous female. Apart from the highest TBTO concentration （60 ng LI）, where all females aborted their egg sacs, 20 ng TBTO L-1 was the only concentration that significantly decreased rm compared with that of control （an effect associated with decreased sex ratio）. The results show that the S. poplesia is affected by prolonged exposure to low concentrations of TBTO. The full life-cycle toxicity test combined with histology experiments provides more integral understanding of the toxicity of endocrine disrupters.展开更多
The non-commercial forest is an important natural resource protecting the environment and contributing to the sustainable development of a nation. Due to its crucial positive external effects, it is inevitable policy ...The non-commercial forest is an important natural resource protecting the environment and contributing to the sustainable development of a nation. Due to its crucial positive external effects, it is inevitable policy that non-commercial forestry should be invested by government, thereby formulates the non-commercial forestry compensation system. This paper first discusses the theoretical basis of non-commercial forestry compensation system and the process of establishment of non-commercial forestry compensation system in China, and then analyzes the deficiency in the current compensation system.展开更多
Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive condition associated with exocrine pancreatic insufficiency,and is characterized by hypoplastic nasal alae,mental retardation,sensorineural hearing loss,s...Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive condition associated with exocrine pancreatic insufficiency,and is characterized by hypoplastic nasal alae,mental retardation,sensorineural hearing loss,short stature,scalp defects,dental abnormalities and abnormal hair patterns. Growth hormone deficiency,hypopituitarism,and impaired glucagon secretion response to insulin-induced hypoglycemia have been reported. Congenital heart defects have also been described in this condition. Mental retardation is typically moderate to severe in patients with JBS; however,normal intelligence can occur. In the pancreas,there is a selective defect of acinar tissue,whereas the islets of Langerhans and ducts are preserved. Diabetes has been reported in older children,suggesting the progressive nature of pancreatic disease. The molecular basis of JBS has recently been mapped to chromosome 15q15-q21 with identified mutations in the UBR1 gene. We report the case of a 7-year-old female with pancreatic insufficiency and mild phenotypic features,in whom the diagnosis of JBS was established using recently described molecular testing for the UBR1 gene.展开更多
Pelagic copepods play an important role in the marine food web. However, a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-bas...Pelagic copepods play an important role in the marine food web. However, a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-based copepod diet studies, we found many apostome ciliates that live symbiotically under the exoskeleton of the copepods, and their sequences were often over-represented in the 18S rRNA gene （18S rDNA） libraries. As a first step to address this issue, we designed three apostome ciliate 18S rDNA blocking primers, and tested their blocking efficiency against apostome ciliate 18S rDNA under various PCR conditions. Using a semi-quantitative PCR method, we optimized the conditions to efficiently amplify the 18S rDNA of the prey while simultaneously excluding the symbiotic apostome ciliates. This technique will facilitate PCR-based diet studies of copepods and other zooplankton in their natural environments.展开更多
Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized fu...Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.展开更多
Considering the compliance control problem of a hexapod robot under different environments, a control strategy based on the improved adaptive control algorithm is proposed. The model of robot structure and impedance c...Considering the compliance control problem of a hexapod robot under different environments, a control strategy based on the improved adaptive control algorithm is proposed. The model of robot structure and impedance control is established. Then, the indirect adaptive control algorithm is derived. Through the analysis of its parameters, it can be noticed that the algorithm does not meet the requirements of the robot compliance control in a complex environment. Therefore, the fuzzy control algorithm is used to adjust the adaptive control parameters. The satisfied system response can be obtained based on the adjustment in real time according to the error between input and output. Comparative experiments and analysis of traditional adaptive control and the improved adaptive control algorithm are presented. It can be verified that not only desired contact force can be reached quickly in different environments, but also smaller contact impact and sliding avoidance are guaranteed, which means that the control strategy has great significance to enhance the adaptability of the hexapod robot.展开更多
A dynamic cooperation model of multi-agent is established by combining reinforcement learning with distributed artificial intelligence(DAI),in which the concept of individual optimization loses its meaning because of ...A dynamic cooperation model of multi-agent is established by combining reinforcement learning with distributed artificial intelligence(DAI),in which the concept of individual optimization loses its meaning because of the dependence of repayment on each agent itself and the choice of other agents.Utilizing the idea of DAI,the intellectual unit of each robot and the change of task and environment,each agent can make decisions independently and finish various complicated tasks by communication and reciprocation between each other.The method is superior to other reinforcement learning methods commonly used in the multi-agent system.It can improve the convergence velocity of reinforcement learning,decrease requirements of computer memory,and enhance the capability of computing and logical ratiocinating for agent.The result of a simulated robot soccer match proves that the proposed cooperative strategy is valid.展开更多
Significant research interest has recently been attracted to the study of bipedal robots due to the wide variety of their potential applications.In reality,bipedal robots are often required to perform gait transitions...Significant research interest has recently been attracted to the study of bipedal robots due to the wide variety of their potential applications.In reality,bipedal robots are often required to perform gait transitions to achieve flexible walking.In this paper,we consider the gait transition of a five-link underactuated three-dimensional(3 D)bipedal robot,and propose a two-layer control strategy.The strategy consists of a unique,event-based,feedback controller whose feedback gain in each step is updated by an adaptive control law,and a transition controller that guides the robot from the current gait to a neighboring point of the target gait so that the state trajectory can smoothly converge to the target gait.Compared with previous works,the transition controller is parameterized and its control parameters are obtained by solving an optimization problem to guarantee the physical constraints in the transition process.Finally,the effectiveness of the control strategy is illustrated on the underactuated 3 D bipedal robot.展开更多
Objective： To observe the clinical efficacy of acupuncture for posterior circulation ischemia vertigo （PCIV）. Methods： A total of 71 PCIV cases were randomly allocated into an acupuncture group （37 cases） and a ...Objective： To observe the clinical efficacy of acupuncture for posterior circulation ischemia vertigo （PCIV）. Methods： A total of 71 PCIV cases were randomly allocated into an acupuncture group （37 cases） and a medication （oral Flunarizine Hydrochloride） group （34 cases）. After 6 weeks of treatment, the therapeutic efficacies in the two groups were analyzed. Results： After treatment, the mean blood flow velocities （VM） of both the vertebral artery （VA） and the basilar artery （BA） in acupuncture group were significantly increased, showing statistical differences when compared with the pre-treatment result of the same group （P〈0.05）; despite some decrease in pulsatility index （PI） after treatment, there was no statistical difference when compared with the pre-treatment result. After treatment, the mean blood flow velocities of both VA and BA in the medication group were increased, showing statistical differences when compared with the pre-treatment result of the same group （P〈0.05）; there was no statistical difference in PI before and after the treatment （P〉0.05）. Regarding the improvement of VM, the acupuncture group was superior to the medication group, showing a statistical difference （P〈0.05）; there was no statistical difference in PI improvement between the two groups. Considering from the clinical efficacy, both acupuncture and medication are effective. However, acupuncture obtained a significantly better effect than medication （P〈0.05）. Conclusion： Acupuncture therapy can posterior circulation ischemia vertigo. obtain a better effect than oral Flunarizine Hydrochloride for展开更多
The complete sequence of the mitochondrial genome of the Japanese snapping shrimp Alpheus japonicus Miers （Crustacea： Decapoda： Caridea） is presented here. A comparative analysis based on the currently available m...The complete sequence of the mitochondrial genome of the Japanese snapping shrimp Alpheus japonicus Miers （Crustacea： Decapoda： Caridea） is presented here. A comparative analysis based on the currently available mitochondrial genomic data re- vealed many previously unknown characteristics of the mitochondrial genomes of caridean shrimps. The A. japonicus mito- chondrial genome is 16487 bp long and contains the typical set of 37 metazoan genes. The gene arrangements in the mito- chondrial genomes of four previously studied carideans （Macrobrachium rosenbergii, M. nipponense, M. lanchesteri and Halocaridina rubra） were found to be identical to the pancrustacean ground pattern; thus, it was considered that gene rear- rangements probably did not occur in the suborder Caridea. In the present study, a translocation of the trnE gene involving in- version was found in Alpheus mitochondrial genomes. This phenomenon has not been reported in any other crustacean mito- chondrial genome that has been studied so far; however, the translocation of one transfer RNA gene （trnP or trnT） was report- ed in the mitochondrial genome of Exopalaemon carinicauda. When the ratios of the nonsynonymous and synonymous sub- stitutions rates （Ka/Ks） for the 13 protein coding genes from two Alpheus species （A. japonicus and A. distinguendus） and three Macrobrachium species （M. rosenbergii, M. nipponense, M. lanchesteri） were calculated, the KaIKs values for all the protein coding genes in Alpheus and Macrobrachium mitochondrial genomes were found to be less than 1 （between 0.0048 and 0.2057）, indicating that a strong purification selection had occurred. The phylogenetic tree that was constructed based on the mitochondrial protein coding genes in the genomes of nine related species indicated that Palaemonidae and Alpheidae formed a monophyly and shared a statistically significant relationship, （Palaemonidae＋Alpheidae）＋Atyidae, at the family level.展开更多
In the present study,we aimed to investigate the effect of puromycin aminonucleoside(PAN)on the expression and distribution of transient receptor potential canonical 6(TRPC6)of murine podocytes and further study the p...In the present study,we aimed to investigate the effect of puromycin aminonucleoside(PAN)on the expression and distribution of transient receptor potential canonical 6(TRPC6)of murine podocytes and further study the protective mechanism of fluvastatin on podocyte injury by TRPC6 in vitro.Podocytes were treated by PAN at different doses and at different time points.The expressions of TRPC6 at mRNA and protein levels were assessed.An immunofluorescent assay was used to observe the distribution of TRPC6.Cultured podocytes were then divided into four groups.The expressions of TRPC6 at mRNA and protein levels were measured.The intracellular calcium concentration of podocytes was measured with a laser-scanning confocal microscope.The paracellular permeability to BSA was evaluated using Millicell-PCF Inserts.The expressions of TRPC6 at mRNA and protein levels were increased in a dose and time-dependent manner after exposure to PAN.Immunofluorescence showed that the expression intensity of TRPC6 was significantly increased,and the distribution of TRPC6 was changed after PAN was applied to podocytes.With fluvastatin intervention,PAN-induced up-regulation of TRPC6 was significantly reversed.The ratio of the peak value of intracellular calcium to the basic calcium value in the PAN group was significantly higher after TRPC6 was activated by OAG,while it is obviously reversed under the action of fluvastatin.The podocyte permeability was significantly increased after 48 h of PAN treatment,while the above situation was effectively improved after fluvastatin intervention.The changes of distribution and expression of TRPC6 were related to podocyte injury induced by PAN.Fluvastatin could exert its protective effects on podocytes by down-regulating TRPC6.展开更多
文摘Two novel hydroperoxylated Lycopodium alkaloids, 11alpha-hydroperoxyphlegmariurine B (1) and 7-hydroperoxyphlegmariurine B (2), along with a known compound, phlegmanurine B (3), were isolated from the total alkaloid fraction of the Chinese medicinal herb Huperzia serrata (Thunb.) Trev. Their structures and relative configurations were elucidated on the basis of spectroscopic analyses.
基金Supported by the National Basic Research Program of China(973 Program)(No.2005CB422300)
文摘A full life-cycle toxicity test, combined with histology, on calanoid copepod Schmackeria poplesia was used to study the effect of bis（tributyltin） oxide （TBTO）. The results indicate no sex-specific differences in TBTO toxicity. Long-term mortalities of the copepods exposed to concentrations higher than 20 ng TBTO L＂1 were significantly elevated compared with that of control, and larval development was inhibited when they were exposed to 40 and 60 ng TBTO L-1. The percentages of ovigerous females were reduced compared with the control （P〈0.01） after 24 days exposure to concentrations higher than 10 ng TBTO L-1. Histological examinations suggest that exposure to TBTO might block the posterior end of the diverticula and inhibits the production of egg sacs. A modified Euler-Lotka equation was used to calculate a population-level endpoint, the intrinsic rate of natural increase （rm）, from individual life-table endpoints, i.e. mortality rate, time of release of first brood, sex ratio, the fraction of ovigerous females among all females as well as the number of nauplii per ovigerous female. Apart from the highest TBTO concentration （60 ng LI）, where all females aborted their egg sacs, 20 ng TBTO L-1 was the only concentration that significantly decreased rm compared with that of control （an effect associated with decreased sex ratio）. The results show that the S. poplesia is affected by prolonged exposure to low concentrations of TBTO. The full life-cycle toxicity test combined with histology experiments provides more integral understanding of the toxicity of endocrine disrupters.
文摘The non-commercial forest is an important natural resource protecting the environment and contributing to the sustainable development of a nation. Due to its crucial positive external effects, it is inevitable policy that non-commercial forestry should be invested by government, thereby formulates the non-commercial forestry compensation system. This paper first discusses the theoretical basis of non-commercial forestry compensation system and the process of establishment of non-commercial forestry compensation system in China, and then analyzes the deficiency in the current compensation system.
基金Supported by Grant from the German Research Foundation (DFG) to MZ
文摘Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive condition associated with exocrine pancreatic insufficiency,and is characterized by hypoplastic nasal alae,mental retardation,sensorineural hearing loss,short stature,scalp defects,dental abnormalities and abnormal hair patterns. Growth hormone deficiency,hypopituitarism,and impaired glucagon secretion response to insulin-induced hypoglycemia have been reported. Congenital heart defects have also been described in this condition. Mental retardation is typically moderate to severe in patients with JBS; however,normal intelligence can occur. In the pancreas,there is a selective defect of acinar tissue,whereas the islets of Langerhans and ducts are preserved. Diabetes has been reported in older children,suggesting the progressive nature of pancreatic disease. The molecular basis of JBS has recently been mapped to chromosome 15q15-q21 with identified mutations in the UBR1 gene. We report the case of a 7-year-old female with pancreatic insufficiency and mild phenotypic features,in whom the diagnosis of JBS was established using recently described molecular testing for the UBR1 gene.
基金Supported by the National Natural Science Foundation of China(No.41076085)
文摘Pelagic copepods play an important role in the marine food web. However, a full understanding of the ecological status of this zooplankton group depends on the careful study of their natural diets. In previous PCR-based copepod diet studies, we found many apostome ciliates that live symbiotically under the exoskeleton of the copepods, and their sequences were often over-represented in the 18S rRNA gene （18S rDNA） libraries. As a first step to address this issue, we designed three apostome ciliate 18S rDNA blocking primers, and tested their blocking efficiency against apostome ciliate 18S rDNA under various PCR conditions. Using a semi-quantitative PCR method, we optimized the conditions to efficiently amplify the 18S rDNA of the prey while simultaneously excluding the symbiotic apostome ciliates. This technique will facilitate PCR-based diet studies of copepods and other zooplankton in their natural environments.
基金the National Natural Science Foundation of China (Nos. 31302215, 31272643)the Shandong Provincial Natural Science Foundation (Nos. BS2014NY010, ZR2013CQ030)the Shandong Provincial Primary Research and Development Projects (No. 2015GNC110017)
文摘Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.
基金Project(51221004) supported by the Science Fund for Creative Research Groups of National Natural Science Foundation of ChinaProject(2010R50036) supported by the Program for Zhejiang Leading Team of S&T Innovation,China
文摘Considering the compliance control problem of a hexapod robot under different environments, a control strategy based on the improved adaptive control algorithm is proposed. The model of robot structure and impedance control is established. Then, the indirect adaptive control algorithm is derived. Through the analysis of its parameters, it can be noticed that the algorithm does not meet the requirements of the robot compliance control in a complex environment. Therefore, the fuzzy control algorithm is used to adjust the adaptive control parameters. The satisfied system response can be obtained based on the adjustment in real time according to the error between input and output. Comparative experiments and analysis of traditional adaptive control and the improved adaptive control algorithm are presented. It can be verified that not only desired contact force can be reached quickly in different environments, but also smaller contact impact and sliding avoidance are guaranteed, which means that the control strategy has great significance to enhance the adaptability of the hexapod robot.
文摘A dynamic cooperation model of multi-agent is established by combining reinforcement learning with distributed artificial intelligence(DAI),in which the concept of individual optimization loses its meaning because of the dependence of repayment on each agent itself and the choice of other agents.Utilizing the idea of DAI,the intellectual unit of each robot and the change of task and environment,each agent can make decisions independently and finish various complicated tasks by communication and reciprocation between each other.The method is superior to other reinforcement learning methods commonly used in the multi-agent system.It can improve the convergence velocity of reinforcement learning,decrease requirements of computer memory,and enhance the capability of computing and logical ratiocinating for agent.The result of a simulated robot soccer match proves that the proposed cooperative strategy is valid.
基金Project supported by the National Natural Science Foundation of China(Nos.91748126,11772292,and 51521064)
文摘Significant research interest has recently been attracted to the study of bipedal robots due to the wide variety of their potential applications.In reality,bipedal robots are often required to perform gait transitions to achieve flexible walking.In this paper,we consider the gait transition of a five-link underactuated three-dimensional(3 D)bipedal robot,and propose a two-layer control strategy.The strategy consists of a unique,event-based,feedback controller whose feedback gain in each step is updated by an adaptive control law,and a transition controller that guides the robot from the current gait to a neighboring point of the target gait so that the state trajectory can smoothly converge to the target gait.Compared with previous works,the transition controller is parameterized and its control parameters are obtained by solving an optimization problem to guarantee the physical constraints in the transition process.Finally,the effectiveness of the control strategy is illustrated on the underactuated 3 D bipedal robot.
基金supported by Acupuncture-moxibustion Hospital Affiliated to Anhui College of Traditional Chinese Medicine
文摘Objective： To observe the clinical efficacy of acupuncture for posterior circulation ischemia vertigo （PCIV）. Methods： A total of 71 PCIV cases were randomly allocated into an acupuncture group （37 cases） and a medication （oral Flunarizine Hydrochloride） group （34 cases）. After 6 weeks of treatment, the therapeutic efficacies in the two groups were analyzed. Results： After treatment, the mean blood flow velocities （VM） of both the vertebral artery （VA） and the basilar artery （BA） in acupuncture group were significantly increased, showing statistical differences when compared with the pre-treatment result of the same group （P〈0.05）; despite some decrease in pulsatility index （PI） after treatment, there was no statistical difference when compared with the pre-treatment result. After treatment, the mean blood flow velocities of both VA and BA in the medication group were increased, showing statistical differences when compared with the pre-treatment result of the same group （P〈0.05）; there was no statistical difference in PI before and after the treatment （P〉0.05）. Regarding the improvement of VM, the acupuncture group was superior to the medication group, showing a statistical difference （P〈0.05）; there was no statistical difference in PI improvement between the two groups. Considering from the clinical efficacy, both acupuncture and medication are effective. However, acupuncture obtained a significantly better effect than medication （P〈0.05）. Conclusion： Acupuncture therapy can posterior circulation ischemia vertigo. obtain a better effect than oral Flunarizine Hydrochloride for
基金supported by the National Natural Science Foundation of China (Grant Nos. 40906067 and 31172054)Special Funds From the Central Finance to Support the Development of Local Universities(Grant No. CXTD04)+1 种基金the China Postdoctoral Science Foundation Funded Project (Grant No. 2012M510054)the Jiangsu Key Laboratory of Marine Biotechnology (Grant Nos. 2009HS12 and 2009HS13)
文摘The complete sequence of the mitochondrial genome of the Japanese snapping shrimp Alpheus japonicus Miers （Crustacea： Decapoda： Caridea） is presented here. A comparative analysis based on the currently available mitochondrial genomic data re- vealed many previously unknown characteristics of the mitochondrial genomes of caridean shrimps. The A. japonicus mito- chondrial genome is 16487 bp long and contains the typical set of 37 metazoan genes. The gene arrangements in the mito- chondrial genomes of four previously studied carideans （Macrobrachium rosenbergii, M. nipponense, M. lanchesteri and Halocaridina rubra） were found to be identical to the pancrustacean ground pattern; thus, it was considered that gene rear- rangements probably did not occur in the suborder Caridea. In the present study, a translocation of the trnE gene involving in- version was found in Alpheus mitochondrial genomes. This phenomenon has not been reported in any other crustacean mito- chondrial genome that has been studied so far; however, the translocation of one transfer RNA gene （trnP or trnT） was report- ed in the mitochondrial genome of Exopalaemon carinicauda. When the ratios of the nonsynonymous and synonymous sub- stitutions rates （Ka/Ks） for the 13 protein coding genes from two Alpheus species （A. japonicus and A. distinguendus） and three Macrobrachium species （M. rosenbergii, M. nipponense, M. lanchesteri） were calculated, the KaIKs values for all the protein coding genes in Alpheus and Macrobrachium mitochondrial genomes were found to be less than 1 （between 0.0048 and 0.2057）, indicating that a strong purification selection had occurred. The phylogenetic tree that was constructed based on the mitochondrial protein coding genes in the genomes of nine related species indicated that Palaemonidae and Alpheidae formed a monophyly and shared a statistically significant relationship, （Palaemonidae＋Alpheidae）＋Atyidae, at the family level.
基金National Natural Science Foundation of China(Grant No.81400333)。
文摘In the present study,we aimed to investigate the effect of puromycin aminonucleoside(PAN)on the expression and distribution of transient receptor potential canonical 6(TRPC6)of murine podocytes and further study the protective mechanism of fluvastatin on podocyte injury by TRPC6 in vitro.Podocytes were treated by PAN at different doses and at different time points.The expressions of TRPC6 at mRNA and protein levels were assessed.An immunofluorescent assay was used to observe the distribution of TRPC6.Cultured podocytes were then divided into four groups.The expressions of TRPC6 at mRNA and protein levels were measured.The intracellular calcium concentration of podocytes was measured with a laser-scanning confocal microscope.The paracellular permeability to BSA was evaluated using Millicell-PCF Inserts.The expressions of TRPC6 at mRNA and protein levels were increased in a dose and time-dependent manner after exposure to PAN.Immunofluorescence showed that the expression intensity of TRPC6 was significantly increased,and the distribution of TRPC6 was changed after PAN was applied to podocytes.With fluvastatin intervention,PAN-induced up-regulation of TRPC6 was significantly reversed.The ratio of the peak value of intracellular calcium to the basic calcium value in the PAN group was significantly higher after TRPC6 was activated by OAG,while it is obviously reversed under the action of fluvastatin.The podocyte permeability was significantly increased after 48 h of PAN treatment,while the above situation was effectively improved after fluvastatin intervention.The changes of distribution and expression of TRPC6 were related to podocyte injury induced by PAN.Fluvastatin could exert its protective effects on podocytes by down-regulating TRPC6.
