odeling · mechanismObjective To investigate mechanisms of anti hypertension and anti cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs) Methods The comparison of ...odeling · mechanismObjective To investigate mechanisms of anti hypertension and anti cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs) Methods The comparison of blood pressure (BP) increment with the ages and cardiovascular changes of SHRs was made between the 3% phe intervented group (SHR phe) and the control SHRs group Detection of the structural changes with the VIDAS digital vedio frequency processing technique and light and electron microscopy were made The cell growth and proliferation of cultured smooth muscle cells (CSMCs) of the thoracic aortas or myocardial fibroblasts were evaluated by measuring the 3 H thymidine counts per minute (cpm) incorporated into the new synthesized desoxyribonucleic acid (DNA) and determining the cell number with the crystal violet stain technique The Ca 2+ influx was measured in counts/min of 45 CaCl 2 after incubating it with 5 different concentrations of phenylalanine and the intracellular [Ca 2+ ] i by Fura Ⅱ/Am indicator The total messenger ribonucleic acid (mRNA) of the myocardium was extracted and Northern blot analysis was performed with the probe collagen α 2(Ⅰ)cDNA The tyrosine hydroxylase (TH) activity was measured by high performance liquid chromatography (HPLC) with electrochemical detector after having reacted with its substrate tyrosine and other reagents The catecholamine contents in brain homogenat were detected by HPLC method The comparison of pharmacokinetics of phenylalanine among SHR phe, SHRs and control Wistar Kyoto (WKY) rats was made after intravenous injection of 3 H L phe (1?ml/kg) by PK GRAPH Program for kinetic calculation The 3H L phe uptake by CSMCs after incubating for difinite intervals was also detected and compared Results Phenylalanine could prevent the increase of BP with ages and the heart weight (heart/body weight index) The aortic media thickness and the collagen content in the myocardium were decreased significantly in SHR phe Whereas the dearranged cardiovascular structure was much improved The mechanisms might be direct and specific inhibition of the DNA synthesis and proliferation of cardiovascular cells which may be related to the inhibition of collagen α 2(Ⅰ)cDNA, c fos and c myc expression Other mechanisms may include decrease of intracellular [Ca 2+ ] i and an inhibition of central sympathetic activity due to the results of higher TH activity in the caudate nucleus and higher adrenaline content in the posterior hypothalamus Besides, partial recovery of phenylalanine metabolic aberrants existed in SHRs seems to be another possibility for its effectiveness Conclusions Phenylalanine intervention could exert a definite anti hypertension and anti cardiovascular remodeling effects on SHRs like seen in human essential hypertension Its mechanisms might be related to direct inhibition of growth in the cardiovascular cells, decrease of central sympathetic activity, the reverse of the exhibited phenylalanine metabolic aberrants in SHRs, and a decrement of intracellular [Ca 2+ ] i展开更多
The numerical thermal mechanical simulation of radial forging process of steel H13 stepped shaft with GFM(Gesellschaft fur Fertigungstechnik und Maschinenbau) forging machine was carried out by three-dimensional finit...The numerical thermal mechanical simulation of radial forging process of steel H13 stepped shaft with GFM(Gesellschaft fur Fertigungstechnik und Maschinenbau) forging machine was carried out by three-dimensional finite element code DEFORM 3D.According to the effective plastic strain,the mean stress and the mean plastic strain distribution of the radial forging,the forging penetration efficiency(FPE) was studied throughout each operation.The results show that the effective plastic strain in the center of the forging is always greater than zero for the desirable larger axial drawing velocity.The mean stress in the center of the workpiece is proposed to describe hydrostatic pressure in this paper.There is compressive strain layer beneath the surface of the workpiece to be found,while there is tensile strain core in the center of the workpiece.These results could be a valuable reference for designing the similar forging operations.展开更多
This review highlights very recent achievements and new developments of severe plastic deformation(SPD) technology for producing bulk ultrafine-grain(UFG) and even nanocrystalline(nc) materials.These numerous modified...This review highlights very recent achievements and new developments of severe plastic deformation(SPD) technology for producing bulk ultrafine-grain(UFG) and even nanocrystalline(nc) materials.These numerous modified and novel SPD methods include cyclic forward-backward extrusion,axi-symmetric forward spiral extrusion,vortex extrusion,simple shear extrusion,planar twist extrusion,tubular channel angular pressing,cone-cone method,high-pressure tube twisting,tube channel pressing and elliptical cross-section spiral equal-channel extrusion.According to classification,these new methods are categorized into the extension of equal-channel angle pressing(ECAP),high-pressure torsion(HPT),twist extrusion(TE) and constrained groove pressing(CGP),respectively.The principles of various new SPD technologies are described in detail.In addition,the microstructure revolution characteristics and mechanical properties of materials produced by SPD process,as well as the applications of SPD techniques to UFG materials,are also reported.Furthermore,this article reviews recent progresses in determining the refinement and/or deformation mechanisms,e.g.dislocation deformation mechanism,twin deformation mechanism and grain boundary sliding and torsional deformation mechanism,and further orientation of SPD technology.展开更多
基金ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9173 5 0andNo 3 9470 62 6)
文摘odeling · mechanismObjective To investigate mechanisms of anti hypertension and anti cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs) Methods The comparison of blood pressure (BP) increment with the ages and cardiovascular changes of SHRs was made between the 3% phe intervented group (SHR phe) and the control SHRs group Detection of the structural changes with the VIDAS digital vedio frequency processing technique and light and electron microscopy were made The cell growth and proliferation of cultured smooth muscle cells (CSMCs) of the thoracic aortas or myocardial fibroblasts were evaluated by measuring the 3 H thymidine counts per minute (cpm) incorporated into the new synthesized desoxyribonucleic acid (DNA) and determining the cell number with the crystal violet stain technique The Ca 2+ influx was measured in counts/min of 45 CaCl 2 after incubating it with 5 different concentrations of phenylalanine and the intracellular [Ca 2+ ] i by Fura Ⅱ/Am indicator The total messenger ribonucleic acid (mRNA) of the myocardium was extracted and Northern blot analysis was performed with the probe collagen α 2(Ⅰ)cDNA The tyrosine hydroxylase (TH) activity was measured by high performance liquid chromatography (HPLC) with electrochemical detector after having reacted with its substrate tyrosine and other reagents The catecholamine contents in brain homogenat were detected by HPLC method The comparison of pharmacokinetics of phenylalanine among SHR phe, SHRs and control Wistar Kyoto (WKY) rats was made after intravenous injection of 3 H L phe (1?ml/kg) by PK GRAPH Program for kinetic calculation The 3H L phe uptake by CSMCs after incubating for difinite intervals was also detected and compared Results Phenylalanine could prevent the increase of BP with ages and the heart weight (heart/body weight index) The aortic media thickness and the collagen content in the myocardium were decreased significantly in SHR phe Whereas the dearranged cardiovascular structure was much improved The mechanisms might be direct and specific inhibition of the DNA synthesis and proliferation of cardiovascular cells which may be related to the inhibition of collagen α 2(Ⅰ)cDNA, c fos and c myc expression Other mechanisms may include decrease of intracellular [Ca 2+ ] i and an inhibition of central sympathetic activity due to the results of higher TH activity in the caudate nucleus and higher adrenaline content in the posterior hypothalamus Besides, partial recovery of phenylalanine metabolic aberrants existed in SHRs seems to be another possibility for its effectiveness Conclusions Phenylalanine intervention could exert a definite anti hypertension and anti cardiovascular remodeling effects on SHRs like seen in human essential hypertension Its mechanisms might be related to direct inhibition of growth in the cardiovascular cells, decrease of central sympathetic activity, the reverse of the exhibited phenylalanine metabolic aberrants in SHRs, and a decrement of intracellular [Ca 2+ ] i
文摘The numerical thermal mechanical simulation of radial forging process of steel H13 stepped shaft with GFM(Gesellschaft fur Fertigungstechnik und Maschinenbau) forging machine was carried out by three-dimensional finite element code DEFORM 3D.According to the effective plastic strain,the mean stress and the mean plastic strain distribution of the radial forging,the forging penetration efficiency(FPE) was studied throughout each operation.The results show that the effective plastic strain in the center of the forging is always greater than zero for the desirable larger axial drawing velocity.The mean stress in the center of the workpiece is proposed to describe hydrostatic pressure in this paper.There is compressive strain layer beneath the surface of the workpiece to be found,while there is tensile strain core in the center of the workpiece.These results could be a valuable reference for designing the similar forging operations.
基金supported by the Aeronautical Science Foundation of China(Grant No. 2011ZE53059)the Graduate Starting Seed Fund of Northwestern Polytechnical University (Grant No. Z2011006)
文摘This review highlights very recent achievements and new developments of severe plastic deformation(SPD) technology for producing bulk ultrafine-grain(UFG) and even nanocrystalline(nc) materials.These numerous modified and novel SPD methods include cyclic forward-backward extrusion,axi-symmetric forward spiral extrusion,vortex extrusion,simple shear extrusion,planar twist extrusion,tubular channel angular pressing,cone-cone method,high-pressure tube twisting,tube channel pressing and elliptical cross-section spiral equal-channel extrusion.According to classification,these new methods are categorized into the extension of equal-channel angle pressing(ECAP),high-pressure torsion(HPT),twist extrusion(TE) and constrained groove pressing(CGP),respectively.The principles of various new SPD technologies are described in detail.In addition,the microstructure revolution characteristics and mechanical properties of materials produced by SPD process,as well as the applications of SPD techniques to UFG materials,are also reported.Furthermore,this article reviews recent progresses in determining the refinement and/or deformation mechanisms,e.g.dislocation deformation mechanism,twin deformation mechanism and grain boundary sliding and torsional deformation mechanism,and further orientation of SPD technology.