We demonstrated the simple and effective production of transgenic chickens, in which the enhanced green fluorescence protein (EGFP) was expressed by using third-generation self-inactive HIV-based lentiviral vectors....We demonstrated the simple and effective production of transgenic chickens, in which the enhanced green fluorescence protein (EGFP) was expressed by using third-generation self-inactive HIV-based lentiviral vectors. In our experiments, lentiviruses were injected into 204 fertilized eggs, from which 30 (15%) chickens were hatched. The exogenous gene was detected in the genomes of 16 out of 30 (53%) chickens. The green fluorescence signal was observed directly in various body parts, and was particularly significant in the testes. The transgenes were also found in the offspring of these chickens. The results indicate that HIV-based lentiviral vectors can be used to generate transgenic birds economically and effectively [ Current Zoology 55 (5): 383 - 387,2009].展开更多
AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 genotypes.METHODS:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control...AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 genotypes.METHODS:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control probes were designed,constructed and arrayed on an optical biosensor to develop a microarray assay.Two sets of primers were used in a one tube polymerase chain reaction(PCR) system to amplify two target fragments simultaneously.The biosensor microarray contained probes that had been sequenced to confirm that they included the rs8099917C/T or rs12979860G/T alleles of interest and could serve as the specific assay standards.In addition to rehybridization of four probes of known sequence,a total of 40 clinical samples collected from hepatitis C seropositive patients were also tested.The target fragments of all 40 samples were amplified in a 50 μL PCR system.Ten μL of each amplicon was tested by BBM assay,and another 40 μL was used for sequencing.The agreement of the results obtained by the two methods was tested statistically using the kappa coefficient.The sensitivity of the BBM assay was evaluated using serial dilutions of ten clinical blood samples containing 10 3-10 4 white cells/μL.RESULTS:As shown by polyacrylamide gel electrophoresis,two target segments of the interleukin 28Bassociated polymorphisms(SNPs) were successfully amplified in the one-tube PCR system.The lengths of the two amplified fragments were consistent with the known length of the target sequences,137 and 159 bps.After hybridization of the PCR amplicons with the probes located on the BBM array,the signals of each allele of both the rs8099917 SNPs and rs12979860 SNPs were observed simultaneously and were clearly visible by the unaided eye.The signals were distinct from each other,could be interpreted visually,and accurately recorded using an ordinary digital camera.To evaluate the specificity of the assay,both the plasmids and clinical samples were applied to the microarray.First,30 PCR amplicons of the various SNP alleles were hybridized on the BBM microarray.Full agreement between plasmids and the BBM assay was observed,with 30/30 correct matches(100%).The kappa value for the BBM assay with plasmids was 1.00(P < 0.05).For the 40 clinical blood samples,the BBM assay hybridization and direct sequencing results were compared for each amplicon.For patient blood samples,agreement was 28/28 for rs8099917T/T,9/11 for rs8099917T/G,1/1 for rs8099917G/G,24/24 for rs12979860C/C,11/14 for rs12979860C/T,and 2/2 for rs12979860T/T.Only five clinical samples of amplicon assay and direct sequencing results were discordant and heterozygotes:2/11 rs8099917T/G and 3/14 rs12979860C/T.The agreement of outcomes between BBM assay and direct sequencing for the detection of rs8099917 and rs12979860 was 95% and 92.5%,respectively;and the corresponding kappa values were 0.88 and 0.85(A kappa value > 0.75 was defined as substantial agreement).The BBM assay and sequencing had similar specificities for detection and identification of the two SNPs and their alleles.The sensitivity evaluation showed that the BBM assay could detect and identify SNP sequences present in blood samples containing as few as 10 2 white blood cells/μL.CONCLUSION:This biosensor microarray assay was highly specific,sensitive,rapid and easy to perform.It is compatible with clinical practice for detection of rs8099917 and rs12979860.展开更多
In the evolution of economic growth drivers, technology gaps are a key variable that determines the efficiency of resource allocation. Analysis of an optimal resource allocation path based on an extended endogenous gr...In the evolution of economic growth drivers, technology gaps are a key variable that determines the efficiency of resource allocation. Analysis of an optimal resource allocation path based on an extended endogenous growth model reveals that economic growth drivers evolve from productive investment to R&D investment and a shift from imitation to innovation. Empirical analysis based on China's provincial-level panel data suggests that the effect of productive investment and R&D investment, as well as innovation and imitation, on economic growth and technological progress varies greatly among regions of disparate technology levels. As a late-starting country, China should properly allocate resources between productive investment and R&D investment, and between imitational investment and innovative investment while advancing the transformation of economic growth patterns on a differentiated basis in light of regional technology disparities.展开更多
China's transition to sustainable growth model in the coming years depends on the ability of its industrial sector to adapt and innovate. In the llth Five-Year Plan period, China entered the intermediate stage of ind...China's transition to sustainable growth model in the coming years depends on the ability of its industrial sector to adapt and innovate. In the llth Five-Year Plan period, China entered the intermediate stage of industrialization and crossed the threshold to become a middle-income country. China's current industrial structure, therefore, which is still characteristic of early-stage industrialization, must transition to support intermediate and advanced industrialization. Although resource constraints will pose certain hurdles for this transition, the pressure of these constraints will ultimately serve to catalyze, rather than impede, sustainable industrial growth. Furthermore, industrial transition and upgrade will mean not only changes for the nation's industrial structure as a whole but also rethinking the strategic possibilities and direction of industry at the enterprise level. The key to success rests on China's ability to improve indigenous development and boost competitive advantage in international markets through the use of new, advanced technologies.展开更多
This paper employs a stochastic endogenous growth model with productive government expenditure in a small open economy to analyze the optimal fiscal policy. First, a stochastic model of a small open economy is constru...This paper employs a stochastic endogenous growth model with productive government expenditure in a small open economy to analyze the optimal fiscal policy. First, a stochastic model of a small open economy is constructed. Second, the equilibrium solutions of the representative agent's stochastic optimization problem are derived. Third, we obtain the equilibrium solutions of the central planner's stochastic optimization problem and the optimal government expenditure policy. Finally, the optimal tax policy is characterized.展开更多
基金supported by National High Technology Research and Development Program of China(863 Key Program,No.2007AA100504)Anhui Natural Science Foundation(No.050410201)
文摘We demonstrated the simple and effective production of transgenic chickens, in which the enhanced green fluorescence protein (EGFP) was expressed by using third-generation self-inactive HIV-based lentiviral vectors. In our experiments, lentiviruses were injected into 204 fertilized eggs, from which 30 (15%) chickens were hatched. The exogenous gene was detected in the genomes of 16 out of 30 (53%) chickens. The green fluorescence signal was observed directly in various body parts, and was particularly significant in the testes. The transgenes were also found in the offspring of these chickens. The results indicate that HIV-based lentiviral vectors can be used to generate transgenic birds economically and effectively [ Current Zoology 55 (5): 383 - 387,2009].
文摘AIM:To evaluate a novel biosensor-based microarray(BBM) assay for detecting rs12979860 and rs8099917 genotypes.METHODS:Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control probes were designed,constructed and arrayed on an optical biosensor to develop a microarray assay.Two sets of primers were used in a one tube polymerase chain reaction(PCR) system to amplify two target fragments simultaneously.The biosensor microarray contained probes that had been sequenced to confirm that they included the rs8099917C/T or rs12979860G/T alleles of interest and could serve as the specific assay standards.In addition to rehybridization of four probes of known sequence,a total of 40 clinical samples collected from hepatitis C seropositive patients were also tested.The target fragments of all 40 samples were amplified in a 50 μL PCR system.Ten μL of each amplicon was tested by BBM assay,and another 40 μL was used for sequencing.The agreement of the results obtained by the two methods was tested statistically using the kappa coefficient.The sensitivity of the BBM assay was evaluated using serial dilutions of ten clinical blood samples containing 10 3-10 4 white cells/μL.RESULTS:As shown by polyacrylamide gel electrophoresis,two target segments of the interleukin 28Bassociated polymorphisms(SNPs) were successfully amplified in the one-tube PCR system.The lengths of the two amplified fragments were consistent with the known length of the target sequences,137 and 159 bps.After hybridization of the PCR amplicons with the probes located on the BBM array,the signals of each allele of both the rs8099917 SNPs and rs12979860 SNPs were observed simultaneously and were clearly visible by the unaided eye.The signals were distinct from each other,could be interpreted visually,and accurately recorded using an ordinary digital camera.To evaluate the specificity of the assay,both the plasmids and clinical samples were applied to the microarray.First,30 PCR amplicons of the various SNP alleles were hybridized on the BBM microarray.Full agreement between plasmids and the BBM assay was observed,with 30/30 correct matches(100%).The kappa value for the BBM assay with plasmids was 1.00(P < 0.05).For the 40 clinical blood samples,the BBM assay hybridization and direct sequencing results were compared for each amplicon.For patient blood samples,agreement was 28/28 for rs8099917T/T,9/11 for rs8099917T/G,1/1 for rs8099917G/G,24/24 for rs12979860C/C,11/14 for rs12979860C/T,and 2/2 for rs12979860T/T.Only five clinical samples of amplicon assay and direct sequencing results were discordant and heterozygotes:2/11 rs8099917T/G and 3/14 rs12979860C/T.The agreement of outcomes between BBM assay and direct sequencing for the detection of rs8099917 and rs12979860 was 95% and 92.5%,respectively;and the corresponding kappa values were 0.88 and 0.85(A kappa value > 0.75 was defined as substantial agreement).The BBM assay and sequencing had similar specificities for detection and identification of the two SNPs and their alleles.The sensitivity evaluation showed that the BBM assay could detect and identify SNP sequences present in blood samples containing as few as 10 2 white blood cells/μL.CONCLUSION:This biosensor microarray assay was highly specific,sensitive,rapid and easy to perform.It is compatible with clinical practice for detection of rs8099917 and rs12979860.
文摘In the evolution of economic growth drivers, technology gaps are a key variable that determines the efficiency of resource allocation. Analysis of an optimal resource allocation path based on an extended endogenous growth model reveals that economic growth drivers evolve from productive investment to R&D investment and a shift from imitation to innovation. Empirical analysis based on China's provincial-level panel data suggests that the effect of productive investment and R&D investment, as well as innovation and imitation, on economic growth and technological progress varies greatly among regions of disparate technology levels. As a late-starting country, China should properly allocate resources between productive investment and R&D investment, and between imitational investment and innovative investment while advancing the transformation of economic growth patterns on a differentiated basis in light of regional technology disparities.
文摘China's transition to sustainable growth model in the coming years depends on the ability of its industrial sector to adapt and innovate. In the llth Five-Year Plan period, China entered the intermediate stage of industrialization and crossed the threshold to become a middle-income country. China's current industrial structure, therefore, which is still characteristic of early-stage industrialization, must transition to support intermediate and advanced industrialization. Although resource constraints will pose certain hurdles for this transition, the pressure of these constraints will ultimately serve to catalyze, rather than impede, sustainable industrial growth. Furthermore, industrial transition and upgrade will mean not only changes for the nation's industrial structure as a whole but also rethinking the strategic possibilities and direction of industry at the enterprise level. The key to success rests on China's ability to improve indigenous development and boost competitive advantage in international markets through the use of new, advanced technologies.
基金This research is supported by the National Natural Science Foundation of China (No. 70271069).
文摘This paper employs a stochastic endogenous growth model with productive government expenditure in a small open economy to analyze the optimal fiscal policy. First, a stochastic model of a small open economy is constructed. Second, the equilibrium solutions of the representative agent's stochastic optimization problem are derived. Third, we obtain the equilibrium solutions of the central planner's stochastic optimization problem and the optimal government expenditure policy. Finally, the optimal tax policy is characterized.