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无序数组的最大有序序列
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作者 秦沿海 蔡勇 《西南民族学院学报(自然科学版)》 2002年第1期11-16,共6页
从现实世界的游戏规则出发,讨论在不打破原向量本身次序的基础上寻求一个最大的有序序列的算法问题(可能同时存在多个增序(或降序)序列,但本文讨论增序问题).
关键词 二叉树 路径树 Trace函数 数组 最大有序列 算法 增序序列 C++ 设计
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PCR AMPLIFICATION, MOLECULAR CLONING,DNA SEQUENCE ANALYSIS AND IMMUNO/PROTECTION IN BALB/C MICE OF THE 33 kDa ENDOFLAGELLAR PROTEIN OF L.INTERRORGANS SEROVAR LAI
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作者 戴保民 陈庄 +2 位作者 阎和平 赵慧业 李胜富 《Chinese Medical Sciences Journal》 CAS CSCD 1997年第1期15-21,共7页
A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment... A pair of oligonucleotide primers were designed to amplify the endoflagella gene of L. Interrogans serovar lai. An approximately 840 bp fragment was generated with PCR and inserted into plasmid pUC8,after the fragment and pUC8 were digested respectively with BamHI and PstI. A recombinant plasmid (designated as pLF1 ) was obtained. SDS-PAGE analysis indicated that a 33 kDa was expressed in E. Coli JM103 harboring pLF1 and the expression level of the protein was 11 % of the total bacterial soluble proteins. Western blot analysis showed that the protein band could be recognized by the antiserum against the endoflegella (Axial filament ) of Leptospira interrt,gans serover lai. Nucleotide sequence data showed an open reading frame encoding 282 aminoacids residues,corresponding to a protein of molecular weight 33. 6kDa. Comparison of the deduced endoflagellar subunit protein (flaB) amino acid sequence with flagellins from other bacteria revealed a high level of identity with the Trehoema pallid um flaB proteins. Immunization/protection experiment was performed on the model of BALB/C mice and showed that there was higher survival rate in the group JM103-pLE1 than in the group JM103-pUC8. 展开更多
关键词 LEPTOSPIRA endoflagellin sequencing
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从真菌全基因组中筛选丛赤壳科的DNA条形码 被引量:6
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作者 曾昭清 赵鹏 +2 位作者 罗晶 庄文颖 余知和 《中国科学:生命科学》 CSCD 北大核心 2012年第1期55-63,共9页
将粗糙脉孢菌(Neurospora crassa)的注释基因分别与30种丝状子囊菌基因组比较,根据E值大小预测同源序列,从中选择Hsp90,AAC,CDC48和EF3作为候选基因,以丛赤壳科(Nectriaceae)13个属34个概念清晰的种为材料,对215个序列片段采用不同方法... 将粗糙脉孢菌(Neurospora crassa)的注释基因分别与30种丝状子囊菌基因组比较,根据E值大小预测同源序列,从中选择Hsp90,AAC,CDC48和EF3作为候选基因,以丛赤壳科(Nectriaceae)13个属34个概念清晰的种为材料,对215个序列片段采用不同方法进行分析,筛选适合于该科的DNA条形码.将种内与种间序列差异以及序列获得的难易程度作为评价指标.结果表明,Hsp90和AAC基因虽然具有较高的PCR扩增与测序成功率,但种内、种间距离频率分布存在重叠,可能导致部分种的鉴定错误;CDC48基因可以恰当地区分种内与种间差异,但扩增与测序成功率相对较低;EF3基因不存在种内、种间遗传距离重叠,序列容易获得,适合作为该科真菌的DNA条形码. 展开更多
关键词 E值同源基因序列PCR扩与测成功率序列分析
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