遗传免疫或将免疫抗原编码的 DNA 直接导入动物体内是开发更好病毒疫苗的最新技术方案之一。导入 DNA 产生低水平的表达,随后诱导宿主抗原特异性免疫。就病毒性疫苗而言,这种方法有很多突出的优点:1.病毒抗原被宿主细胞适当地折叠和糖基...遗传免疫或将免疫抗原编码的 DNA 直接导入动物体内是开发更好病毒疫苗的最新技术方案之一。导入 DNA 产生低水平的表达,随后诱导宿主抗原特异性免疫。就病毒性疫苗而言,这种方法有很多突出的优点:1.病毒抗原被宿主细胞适当地折叠和糖基化:2.抗原的存在诱导了宿主的体液和细胞免疫应答;3.展开更多
Intravenous anesthetics are known to cause amnesia, but the underlying molecular mechanisms remain elusive. To identify a possible molecular mechanism, we recently turned our attention to a key intracellular signaling...Intravenous anesthetics are known to cause amnesia, but the underlying molecular mechanisms remain elusive. To identify a possible molecular mechanism, we recently turned our attention to a key intracellular signaling pathway organized by a family of mitogen-activated protein kinases （MAPKs）. As a prominent synapse-to-nucleus superhighway, MAPKs couple surface glutamate receptors to nuclear transcriptional events essential for the development and/or maintenance of different forms of synaptic plasticity （long-term potentiation and long-term depression） and memory formation. To define the role of MAPK-dependent transcription in the amnesic property of anesthetics, we conducted a series of studies to examine the effect of a prototype intravenous anesthetic propofol on the MAPK response to N-methyl-D-aspartate receptor （NMDAR） stimulation in hippocampal neurons. Our results suggest that propofol possesses the ability to inhibit NMDAR-mediated activation of a classic subclass of MAPKs, extracellular signal-regulated protein kinase 1/2 （ERK1/2）. Concurrent inhibition of transcriptional activity also occurs as a result of inhibited responses of ERK1/2 to NMDA. These findings provide first evidence for an inhibitory modulation of the NMDAR-MAPK pathway by an intravenous anesthetic and introduce a new avenue to elucidate a transcription-dependent mechanism processing the amnesic effect of anesthetics.展开更多
FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage spe...FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+ cells have higher binding activity to FT Ⅰ and express the proteins which could form the unique DNA-protein com-plex(es) of FT Ⅰ;. 3, The essential sequence for the specific DNA-protein interactions of FT Ⅰ is AAAAGGGGAAGC; 4, South-western analysis in conjunction with the compe-tition assay of the proteins binding to FT Ⅰ, has revealed a 28 kd protein in WEHI 3BD+ cells that displays the properties of the putative transcription factor which acts through FT Ⅰ. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT Ⅰ.展开更多
Thermal effects are incorporated into developed discrete layer mechanics for two-dimensional cylindrical shells structures. Finite element equations are developed according to layerwise theory of laminated structure. ...Thermal effects are incorporated into developed discrete layer mechanics for two-dimensional cylindrical shells structures. Finite element equations are developed according to layerwise theory of laminated structure. Following the layerwise theory, a variable kinematic model that incorporates mechanics and thermal conditions is also presented. The new element has a field of displacement compatible with the cylindrical shell element or plate and it can be used as a rigid element for this structural element.ln the laminate model construction, adjacent layers are arranged as bonded layers. The layer has a unique constant thickness that can be different to each layer. The fiber reinforced is used and the fibers in a laminate may be oriented arbitrarily. The shear stress is adopted equal to zero because the thin thickness, on the other hand, the normal stress is maintained in order to ensure the compatibility of stress in material. The previously authors of this methods neglect the implications of thermal effects on cylindrical shells structures. Thermal effects become important when the structure has to operate in either extremely hot or cold temperature environments. These extreme conditions may severely affect the response of structure in two distinct ways： （1） induction of thermal stresses due to differences in the coefficients of thermal expansion between the various composite plies and layers and （2） temperature dependence of the elastic properties. Only a limited amount of work has been reported concerning this topic. All in all, the main contribution of this work is the consideration of this kinematic for cylindrical shells that incorporate mechanics and thermal conditions. In addition, numerical results are presented to demonstrate the capability of the current formulation to represent the behavior of cylindrical shells with these characteristics.展开更多
文摘Intravenous anesthetics are known to cause amnesia, but the underlying molecular mechanisms remain elusive. To identify a possible molecular mechanism, we recently turned our attention to a key intracellular signaling pathway organized by a family of mitogen-activated protein kinases （MAPKs）. As a prominent synapse-to-nucleus superhighway, MAPKs couple surface glutamate receptors to nuclear transcriptional events essential for the development and/or maintenance of different forms of synaptic plasticity （long-term potentiation and long-term depression） and memory formation. To define the role of MAPK-dependent transcription in the amnesic property of anesthetics, we conducted a series of studies to examine the effect of a prototype intravenous anesthetic propofol on the MAPK response to N-methyl-D-aspartate receptor （NMDAR） stimulation in hippocampal neurons. Our results suggest that propofol possesses the ability to inhibit NMDAR-mediated activation of a classic subclass of MAPKs, extracellular signal-regulated protein kinase 1/2 （ERK1/2）. Concurrent inhibition of transcriptional activity also occurs as a result of inhibited responses of ERK1/2 to NMDA. These findings provide first evidence for an inhibitory modulation of the NMDAR-MAPK pathway by an intravenous anesthetic and introduce a new avenue to elucidate a transcription-dependent mechanism processing the amnesic effect of anesthetics.
文摘FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+ cells have higher binding activity to FT Ⅰ and express the proteins which could form the unique DNA-protein com-plex(es) of FT Ⅰ;. 3, The essential sequence for the specific DNA-protein interactions of FT Ⅰ is AAAAGGGGAAGC; 4, South-western analysis in conjunction with the compe-tition assay of the proteins binding to FT Ⅰ, has revealed a 28 kd protein in WEHI 3BD+ cells that displays the properties of the putative transcription factor which acts through FT Ⅰ. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT Ⅰ.
文摘Thermal effects are incorporated into developed discrete layer mechanics for two-dimensional cylindrical shells structures. Finite element equations are developed according to layerwise theory of laminated structure. Following the layerwise theory, a variable kinematic model that incorporates mechanics and thermal conditions is also presented. The new element has a field of displacement compatible with the cylindrical shell element or plate and it can be used as a rigid element for this structural element.ln the laminate model construction, adjacent layers are arranged as bonded layers. The layer has a unique constant thickness that can be different to each layer. The fiber reinforced is used and the fibers in a laminate may be oriented arbitrarily. The shear stress is adopted equal to zero because the thin thickness, on the other hand, the normal stress is maintained in order to ensure the compatibility of stress in material. The previously authors of this methods neglect the implications of thermal effects on cylindrical shells structures. Thermal effects become important when the structure has to operate in either extremely hot or cold temperature environments. These extreme conditions may severely affect the response of structure in two distinct ways： （1） induction of thermal stresses due to differences in the coefficients of thermal expansion between the various composite plies and layers and （2） temperature dependence of the elastic properties. Only a limited amount of work has been reported concerning this topic. All in all, the main contribution of this work is the consideration of this kinematic for cylindrical shells that incorporate mechanics and thermal conditions. In addition, numerical results are presented to demonstrate the capability of the current formulation to represent the behavior of cylindrical shells with these characteristics.
