As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of a...As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of ammonium sulfate precipitation, size exclusion chromatography, and anion exchange chromatography was devel- oped for the purification of eGFP. Based on the proposed procedure, recombinant eGFP with an electrophoretic pu- rity was achieved in combination with an overall yield of 66% and a purification factor of 17.9. The fluorescent spectrometry of purified eGFP and lysate from E. coli strain expressing eGFP exhibited the same wavelength of ex- citation and emission maxima, indicating that the purification procedure did not influence the construct and fluo- rescent characteristics of desired protein. The procedure mentioned was easy to scale up for the purification of large quantities of eGFP.展开更多
Strength properties of laboratory scale lime-based samples enhanced with additives such as nanomaterials(nanofibrillated cellulose,nanosilica,nanoclay,expanded graphite),hemp&glass fibres,hemp shiv and polyvinyl a...Strength properties of laboratory scale lime-based samples enhanced with additives such as nanomaterials(nanofibrillated cellulose,nanosilica,nanoclay,expanded graphite),hemp&glass fibres,hemp shiv and polyvinyl acetate(PVAc)are determined.Samples were cured for 26 days in air at 20℃/60%RH after casting before being oven dried for a further two days at 50℃(28 days total).Results show that the nanomaterials on their own had a mixed effect on the strength although nSiO_(2) as a solo additive performed exceptionally well.The combination of fibres in conjunction with PVAc also greatly enhanced the strength due to increased bond between the fibres and the matrix.In addition,Greenhouse Gas emissions(GHG,kgCO_(2)eq)of an arbitrary block was determined for all composites and compared to the GHG of a commonly used lightweight aerated concrete block.Comparison of the normalised compressive strengths to the different loading conditions as outlined in BS EN 8103 shows that a more widespread use of pre-cast lime composites is possible and without unduly increasing GHG emissions.展开更多
基金Supported by the National Natural Science Foundation of China (No. 20306024, No. 20476082), Tianjin Applied FundamentalResearch Project (No. 0436048-11) and the Program for Changjiang Scholars and Innovative Research Team in University from the Ministry of Education of China.
文摘As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of ammonium sulfate precipitation, size exclusion chromatography, and anion exchange chromatography was devel- oped for the purification of eGFP. Based on the proposed procedure, recombinant eGFP with an electrophoretic pu- rity was achieved in combination with an overall yield of 66% and a purification factor of 17.9. The fluorescent spectrometry of purified eGFP and lysate from E. coli strain expressing eGFP exhibited the same wavelength of ex- citation and emission maxima, indicating that the purification procedure did not influence the construct and fluo- rescent characteristics of desired protein. The procedure mentioned was easy to scale up for the purification of large quantities of eGFP.
基金supported by the Iraqi Ministry of Higher Education and Scientific Research and Iraqi Cultural Attache in London,who supported the research studies for F.J.Khalaf.
文摘Strength properties of laboratory scale lime-based samples enhanced with additives such as nanomaterials(nanofibrillated cellulose,nanosilica,nanoclay,expanded graphite),hemp&glass fibres,hemp shiv and polyvinyl acetate(PVAc)are determined.Samples were cured for 26 days in air at 20℃/60%RH after casting before being oven dried for a further two days at 50℃(28 days total).Results show that the nanomaterials on their own had a mixed effect on the strength although nSiO_(2) as a solo additive performed exceptionally well.The combination of fibres in conjunction with PVAc also greatly enhanced the strength due to increased bond between the fibres and the matrix.In addition,Greenhouse Gas emissions(GHG,kgCO_(2)eq)of an arbitrary block was determined for all composites and compared to the GHG of a commonly used lightweight aerated concrete block.Comparison of the normalised compressive strengths to the different loading conditions as outlined in BS EN 8103 shows that a more widespread use of pre-cast lime composites is possible and without unduly increasing GHG emissions.
