In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH valu...In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH value at 6.0, the terbiumenrofloxacin complex had a characteristic fluorescence peak of Tb3＋ at 545 nm （λex= 328 nm）, which could be used for the determination of enrofloxacin. A simple, rapid and sensitive fluorescence method for the determination of enrofloxacin was thus established, and an optimal reaction condition was selected. Under the optimal reaction condition, there was a good linear relation （r2=0.992 3） between concentration of enrofloxacin in the range of 1.0×10^-1.0×10^-6 g/ml and its fluorescence intensity at 545 nm, with a detection limit of 1.3×10^-9 g/ml; the recovery for enrofloxacin in tablets was 97.7% with a variation coefficient of 1.4%; and for enrofloxacin in fish tissues, the recovery was 79.0%-94.5% with a variation coefficient of 2.0%-7.8%.展开更多
基金Supported by Scientific Research Fund of Hanshan Normal University(511043)~~
文摘In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH value at 6.0, the terbiumenrofloxacin complex had a characteristic fluorescence peak of Tb3＋ at 545 nm （λex= 328 nm）, which could be used for the determination of enrofloxacin. A simple, rapid and sensitive fluorescence method for the determination of enrofloxacin was thus established, and an optimal reaction condition was selected. Under the optimal reaction condition, there was a good linear relation （r2=0.992 3） between concentration of enrofloxacin in the range of 1.0×10^-1.0×10^-6 g/ml and its fluorescence intensity at 545 nm, with a detection limit of 1.3×10^-9 g/ml; the recovery for enrofloxacin in tablets was 97.7% with a variation coefficient of 1.4%; and for enrofloxacin in fish tissues, the recovery was 79.0%-94.5% with a variation coefficient of 2.0%-7.8%.
