AIM: To study the relationship between inflammatory response and liver regeneration (LR) at transcriptional level.METHODS: After partial hepatectomy (PH) of rats, the genes associated with inflammatory response ...AIM: To study the relationship between inflammatory response and liver regeneration (LR) at transcriptional level.METHODS: After partial hepatectomy (PH) of rats, the genes associated with inflammatory response were obtained according to the databases, and the gene expression changes during LR were checked by the Rat Genome 230 2.0 army. RESULTS: Two hundred and thirty-nine genes were associated with liver regeneration. The initial and total expressing gene numbers found in initiation phase (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure-function reconstruction (66-168 h after PH) of liver regeneration were 107, 34, 126, 6 and 107, 92, 233, 145 respectively, showing that the associated genes were mainly triggered at the beginning of liver regeneration, and worked at different phases. According to their expression similarity, these genes were classified into 5 groups: only up-regulated, predominantly up-, only down-, predominantly down-, up- and down-, involving 92, 25, 77, 14 and 31 genes, respectively. The total times of their up- and down-regulated expression were 975 and 494, respectively, demonstrating that the expressions of the majority of genes were increased, and that of a few genes were decreased. Their time relevance was classified into 13 groups, showing that the cellular physiological and biochemical activities were staggered during liver regeneration. According to gene expression patterns, they were classified into 33 types, suggesting that the activities were diverse and complex during liver regeneration. CONCLUSION: Inflammatory response is closelyassociated with liver regeneration, in which 239 LR- associated genes play an important role.展开更多
In rice, 2,4-Dichlorphenoxyacetic acid (MS2) has been considered best for callus induction and combination ofNAA, IAA, ABA (MS3) for somatic embryogenesis. Efficient plant regeneration was observed when MS basal s...In rice, 2,4-Dichlorphenoxyacetic acid (MS2) has been considered best for callus induction and combination ofNAA, IAA, ABA (MS3) for somatic embryogenesis. Efficient plant regeneration was observed when MS basal salts supplied with 3% sorbitol and 3% maltose as carbon sources without hormones (MSac). During this experiment, effect of sodium chloride (NaCl) and abscisic acid (ABA) was assessed on callus growth, plant regeneration and root induction efficiency of rice (Oryza sativa L.) varieties IR-6 and Basmati-370. Callus proliferation rate was highly decreased in both varieties on MS2b (100 mol.m3 NaCl + 0.5 mg'L1 ABA) than MS2a (100 mol'm3 NaCl) cultures significantly. Proline, glycine-betaine and reducing sugars were increased significantly in MS2a and MS2b callusing cultures. However, total proteins were decreased in MS2a, while slightly increased in MS2b. Maximum plant regeneration (9.42 ±0.54 and 10.67 ±0.50 plantlets.callus1) from somatic embryos was observed on MS4c in IR-6 and Basmati-370, while 1.56 ± 0.06 (IR-6) and 0.95 ±0.05 (Basmati-370) plantlets callus1 were observed on MSab (100 mol·m3 NaCl). No plant regeneration was observed on MS4b (100 mol·m3 NaCl + 0.5 mg·L-1 ABA) medium in both varieties. Inhibition of root induction efficiency was high in MSsb (100 mol·m3 NaCl + 0.5 mg·L-1 ABA) than MS5a (100 mol·m3 NaCl) in the stressed cultures (P 〈 0.05). In this experiment, it was concluded that ABA involved in somatic embryogenesis and elevation of NaCl stress, while it causes inhibition of cell's growth as well as its regeneration into plantlets from somatic embryos.展开更多
To investigate the effects of Ginse noside Rb 1 on the proliferation of Schwann cells in culture.Methods: Applying MTT assay and Thymidine incorporation assay, the effects of Ginsenoside Rb 1 on the proliferation of S...To investigate the effects of Ginse noside Rb 1 on the proliferation of Schwann cells in culture.Methods: Applying MTT assay and Thymidine incorporation assay, the effects of Ginsenoside Rb 1 on the proliferation of Schwann cells isolated from the sciatic nerve of adult rat were studied.Results: Ginsenoside Rb 1 (10 μg/ml) significantly induced Sc hwann cell proliferation, the effect was similar to NGF (50 μg/ml). At high con centrations of Ginsenoside Rb 1 (1 mg/ml), the proliferation of Schwann cells w as significantly inhibited. Conclusions: Ginsenoside Rb 1 at the optimal concentrations is found to be effective in inducing the proliferation of Schwann cells, but at hi gher concentrations the drug is cytotoxic for Schwann cells.展开更多
基金Supported by the National Natural Science Foundation of China,No. 30270673
文摘AIM: To study the relationship between inflammatory response and liver regeneration (LR) at transcriptional level.METHODS: After partial hepatectomy (PH) of rats, the genes associated with inflammatory response were obtained according to the databases, and the gene expression changes during LR were checked by the Rat Genome 230 2.0 army. RESULTS: Two hundred and thirty-nine genes were associated with liver regeneration. The initial and total expressing gene numbers found in initiation phase (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure-function reconstruction (66-168 h after PH) of liver regeneration were 107, 34, 126, 6 and 107, 92, 233, 145 respectively, showing that the associated genes were mainly triggered at the beginning of liver regeneration, and worked at different phases. According to their expression similarity, these genes were classified into 5 groups: only up-regulated, predominantly up-, only down-, predominantly down-, up- and down-, involving 92, 25, 77, 14 and 31 genes, respectively. The total times of their up- and down-regulated expression were 975 and 494, respectively, demonstrating that the expressions of the majority of genes were increased, and that of a few genes were decreased. Their time relevance was classified into 13 groups, showing that the cellular physiological and biochemical activities were staggered during liver regeneration. According to gene expression patterns, they were classified into 33 types, suggesting that the activities were diverse and complex during liver regeneration. CONCLUSION: Inflammatory response is closelyassociated with liver regeneration, in which 239 LR- associated genes play an important role.
文摘In rice, 2,4-Dichlorphenoxyacetic acid (MS2) has been considered best for callus induction and combination ofNAA, IAA, ABA (MS3) for somatic embryogenesis. Efficient plant regeneration was observed when MS basal salts supplied with 3% sorbitol and 3% maltose as carbon sources without hormones (MSac). During this experiment, effect of sodium chloride (NaCl) and abscisic acid (ABA) was assessed on callus growth, plant regeneration and root induction efficiency of rice (Oryza sativa L.) varieties IR-6 and Basmati-370. Callus proliferation rate was highly decreased in both varieties on MS2b (100 mol.m3 NaCl + 0.5 mg'L1 ABA) than MS2a (100 mol'm3 NaCl) cultures significantly. Proline, glycine-betaine and reducing sugars were increased significantly in MS2a and MS2b callusing cultures. However, total proteins were decreased in MS2a, while slightly increased in MS2b. Maximum plant regeneration (9.42 ±0.54 and 10.67 ±0.50 plantlets.callus1) from somatic embryos was observed on MS4c in IR-6 and Basmati-370, while 1.56 ± 0.06 (IR-6) and 0.95 ±0.05 (Basmati-370) plantlets callus1 were observed on MSab (100 mol·m3 NaCl). No plant regeneration was observed on MS4b (100 mol·m3 NaCl + 0.5 mg·L-1 ABA) medium in both varieties. Inhibition of root induction efficiency was high in MSsb (100 mol·m3 NaCl + 0.5 mg·L-1 ABA) than MS5a (100 mol·m3 NaCl) in the stressed cultures (P 〈 0.05). In this experiment, it was concluded that ABA involved in somatic embryogenesis and elevation of NaCl stress, while it causes inhibition of cell's growth as well as its regeneration into plantlets from somatic embryos.
文摘To investigate the effects of Ginse noside Rb 1 on the proliferation of Schwann cells in culture.Methods: Applying MTT assay and Thymidine incorporation assay, the effects of Ginsenoside Rb 1 on the proliferation of Schwann cells isolated from the sciatic nerve of adult rat were studied.Results: Ginsenoside Rb 1 (10 μg/ml) significantly induced Sc hwann cell proliferation, the effect was similar to NGF (50 μg/ml). At high con centrations of Ginsenoside Rb 1 (1 mg/ml), the proliferation of Schwann cells w as significantly inhibited. Conclusions: Ginsenoside Rb 1 at the optimal concentrations is found to be effective in inducing the proliferation of Schwann cells, but at hi gher concentrations the drug is cytotoxic for Schwann cells.