目的:探讨胃癌术前CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达的关系。方法:收集榆林市第二医院收治的胃癌患者68例作为观察组,根据肿瘤分期分为早中期胃癌组41例、晚期胃癌组27例;取同期在本院就诊被证实为胃良性...目的:探讨胃癌术前CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达的关系。方法:收集榆林市第二医院收治的胃癌患者68例作为观察组,根据肿瘤分期分为早中期胃癌组41例、晚期胃癌组27例;取同期在本院就诊被证实为胃良性病变的患者50例作为胃良性病变组。采用CT扫描检测三组患者的CT强化率、灌注参数,采用酶联免疫吸附法(ELISA)检测血清肿瘤标志物含量,采用RT-PCR法检测病灶组织增殖基因mRNA表达量。结果:晚期胃癌组的CER、AF、BV、CL水平高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的血清CA72-4、CA19-9、CA125、CEA含量高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的组织CADM1、miRNA-34a、Cystatin M mRNA表达量低于早中期胃癌组、胃良性病变组,Survivin、I2PP2A mRNA表达量高于早中期胃癌组、胃良性病变组(P<0.05)。经Pearson检验发现,胃癌患者的CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达量存在直接相关关系。结论:胃癌术前CT强化率、灌注参数与肿瘤恶性程度直接相关,可作为远期肿瘤诊断及恶性程度判断的可靠手段。展开更多
Objective: To investigate the effects of static magnetic field(SMF) on the viability, adhesion molecule expression of human umbilical vessel endothelial cell. Methods: Magnetic flux intensity was 0. 1 mT, 1 mT, 10 mT....Objective: To investigate the effects of static magnetic field(SMF) on the viability, adhesion molecule expression of human umbilical vessel endothelial cell. Methods: Magnetic flux intensity was 0. 1 mT, 1 mT, 10 mT. Cell viability and proliferation were measured with 3H-TdR and MTT methods; and apoptosis of human umbilical vein endothelial cell (HUVEC) was studied by flow cytometry and transmission electric microscopy. ELISA was used to measure the expression of ICAM-1 and VCAM-1 on endothelium. Results: 0. 1 mT SMF had no effects on the growth of HUVEC. however,SMF of 1 mT, 10 mT attenuated growth of HUVEC. 10 mT static magnetic field could induce apoptosis and necrosis of HUVEC. 10 mT SMF enhanced the expression of ICAM-1 and VCAM-1 on endothelium. Conclusion: The effect of SMF depends on the intensity of SMF. 10 mT SMF has adverse effects on human umbilical vessel endothelial cell.展开更多
Objective: The aim of this study was to explore the effects on malignant proliferation of A549 cell by silencing cy-clooxygenase (COX)-2. Methods: In the present study, we constructed three siRNA vectors producing sma...Objective: The aim of this study was to explore the effects on malignant proliferation of A549 cell by silencing cy-clooxygenase (COX)-2. Methods: In the present study, we constructed three siRNA vectors producing small interference RNA. The siRNA vectors and the vacant vectors were transfected into A549 cell with lipofectamine respectively and the transfected cell strains were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of lung cancer cells were studied by cell growth curve, clonogenic assay and xenograft assays. Results: The siRNA expression vectors produced marked effects in A549 cell but the inhibited effects were different. The effect of psi-10 was best and the mRNA and protein levels of COX-2 reduced 61.2% and 56.2% respectively in A549-si10 cell in contrast to the control. The growth of A549 cell slowed and the colony formation rate reduced after silencing COX-2. In xenograft assays, the growth speeds of tumor became slow and the numbers of tumor reduced after silencing COX-2. Conclusion: The si10 target of COX-2 has the best silencing effect in A549 cell and the best inhibition effect on malignant proliferation of A549 cell in vivo and in vitro.展开更多
MicroRNAs(miRNAs),which contains approximately 22 nt,belong to a small endogenous,non-coding regulatory single-stranded RNA molecules.They are posttranscriptional regulators of gene expression and highly conserved in ...MicroRNAs(miRNAs),which contains approximately 22 nt,belong to a small endogenous,non-coding regulatory single-stranded RNA molecules.They are posttranscriptional regulators of gene expression and highly conserved in evolution.Many researches show that miRNAs involved in many processes,including tumor formation,cell proliferation and apoptosis and proliferation and metastasis of cancer cells.Among that,the relationship between miRNAs and lung cancer is one of the most focal areas for the researchers,because the abnormal expressions of miRNAs were significantly associated with the occurrence and development of lung cancer.The expression level of different miRNAs in lung cancer cells exist differences,compared with normal lung tissue cells,there are two classes of expression:over-expression level and low expression level.In this review,we focused on studying the mechanism of overexpression miRNAs in lung cancer.展开更多
The aim of this work was to study the ontogeny of chondrocyte cell division using embryo, adult and osteoarthritic(OA) cartilage. We searched for mitosis phases and performed a comparative evaluation of mitotic index,...The aim of this work was to study the ontogeny of chondrocyte cell division using embryo, adult and osteoarthritic(OA) cartilage. We searched for mitosis phases and performed a comparative evaluation of mitotic index, basic fibro-blast growth factor b (FGFb), transforming growth factor β1 (TGF-β1) receptors, cyclin dependent kinase (CDK1)and Cyclin-B expression in fetal, neonate, 3, 5, 8 weeks old rats and experimental OA. Our results showed that mitosisphases were observed in all normal cartilage studied, although, we found a decrease in mitotic index in relation to tissuedevelopment. No mitosis was detected in OA cartilage. We also found a statistical significant reduction in cell number inOA cartilage, compared with the normal tissue. Furthermore, FGFb and TGF-β1 receptors diminished in relation totissue development, and were very scarce in experimental OA. Western blot assays showed CDK-1 expression in allcases, including human-OA cartilage. Similar results were observed for Cyclin-B, except for 8 weeks, when it was notexpressed. Our results suggest that cell division seems to be scarce, if not absent within the OA cartilage studied.Nevertheless, the existence of factors essential for cell division leaves open the question concerning chondrocyteproliferation in OA cartilage, which is likely to be present in the early stages of the disease.展开更多
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomar...Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.展开更多
Objective To explore the mechanism of An-Pressing manipulation in relieving energy crisis in chronic myofascial trigger points(MTrPs)by observing the effects of An-Pressing manipulation on adenosine triphosphate(ATP),...Objective To explore the mechanism of An-Pressing manipulation in relieving energy crisis in chronic myofascial trigger points(MTrPs)by observing the effects of An-Pressing manipulation on adenosine triphosphate(ATP),adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)/peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α)pathway and mitochondrial ultrastructure of skeletal muscle cells in MTrPs rats.Methods Forty-eight male Sprague-Dawley rats were randomly divided into a blank group,a model group,a lidocaine group,and an An-Pressing manipulation group,with 12 rats in each group.The model group,lidocaine group and An-Pressing manipulation group were used to replicate the MTrPs rat model by blunt shock and centrifugal motion method.After modeling,the An-Pressing manipulation group was subjected to 7 times An-Pressing manipulation,once every other day;the lidocaine group was treated with 3 times of injection of lidocaine at the MTrPs,once every 6 d.The blank group and the model group were fed normally without intervention.After the intervention,local muscle tissue was taken to detect the content of ATP and the expression of AMPK,phosphorylated AMPK(phospho-AMPK),PGC-1α,and glucose transporter 4(GluT4),and the ultrastructure of mitochondria was observed under an electron microscope.Results Compared with the blank group,the ATP content in the model group was decreased(P<0.05),the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were decreased(P<0.05);under the electron microscope,the number of mitochondria decreased,and they were deformed,small in volume,and had deformed cristae.Compared with the model group,the ATP contents in the An-Pressing manipulation group and the lidocaine group were increased(P<0.05),and the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were increased(P<0.05);under the electron microscope,the number of mitochondria increased,the shape and size of the mitochondria were basically normal,and the cristae could be seen.Compared with the lidocaine group,phospho-AMPK and the ratio of phospho-AMPK to AMPK in the An-Pressing manipulation group were increased(P<0.05);under the electron microscope,the numbers of mitochondria were similar,and the shape and size of the mitochondria were basically normal without swelling,and the cristae could be observed.Conclusion An-Pressing manipulation can increase the ATP content in MTrPs tissue,improve the expression levels of PGC-1α and GluT4 proteins and the ratio of phospho-AMPK to AMPK;its mechanism may relate to the activation of AMPK/PGC-1α signaling pathway to promote the repair of mitochondrial damages.展开更多
文摘目的:探讨胃癌术前CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达的关系。方法:收集榆林市第二医院收治的胃癌患者68例作为观察组,根据肿瘤分期分为早中期胃癌组41例、晚期胃癌组27例;取同期在本院就诊被证实为胃良性病变的患者50例作为胃良性病变组。采用CT扫描检测三组患者的CT强化率、灌注参数,采用酶联免疫吸附法(ELISA)检测血清肿瘤标志物含量,采用RT-PCR法检测病灶组织增殖基因mRNA表达量。结果:晚期胃癌组的CER、AF、BV、CL水平高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的血清CA72-4、CA19-9、CA125、CEA含量高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的组织CADM1、miRNA-34a、Cystatin M mRNA表达量低于早中期胃癌组、胃良性病变组,Survivin、I2PP2A mRNA表达量高于早中期胃癌组、胃良性病变组(P<0.05)。经Pearson检验发现,胃癌患者的CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达量存在直接相关关系。结论:胃癌术前CT强化率、灌注参数与肿瘤恶性程度直接相关,可作为远期肿瘤诊断及恶性程度判断的可靠手段。
文摘Objective: To investigate the effects of static magnetic field(SMF) on the viability, adhesion molecule expression of human umbilical vessel endothelial cell. Methods: Magnetic flux intensity was 0. 1 mT, 1 mT, 10 mT. Cell viability and proliferation were measured with 3H-TdR and MTT methods; and apoptosis of human umbilical vein endothelial cell (HUVEC) was studied by flow cytometry and transmission electric microscopy. ELISA was used to measure the expression of ICAM-1 and VCAM-1 on endothelium. Results: 0. 1 mT SMF had no effects on the growth of HUVEC. however,SMF of 1 mT, 10 mT attenuated growth of HUVEC. 10 mT static magnetic field could induce apoptosis and necrosis of HUVEC. 10 mT SMF enhanced the expression of ICAM-1 and VCAM-1 on endothelium. Conclusion: The effect of SMF depends on the intensity of SMF. 10 mT SMF has adverse effects on human umbilical vessel endothelial cell.
文摘Objective: The aim of this study was to explore the effects on malignant proliferation of A549 cell by silencing cy-clooxygenase (COX)-2. Methods: In the present study, we constructed three siRNA vectors producing small interference RNA. The siRNA vectors and the vacant vectors were transfected into A549 cell with lipofectamine respectively and the transfected cell strains were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of lung cancer cells were studied by cell growth curve, clonogenic assay and xenograft assays. Results: The siRNA expression vectors produced marked effects in A549 cell but the inhibited effects were different. The effect of psi-10 was best and the mRNA and protein levels of COX-2 reduced 61.2% and 56.2% respectively in A549-si10 cell in contrast to the control. The growth of A549 cell slowed and the colony formation rate reduced after silencing COX-2. In xenograft assays, the growth speeds of tumor became slow and the numbers of tumor reduced after silencing COX-2. Conclusion: The si10 target of COX-2 has the best silencing effect in A549 cell and the best inhibition effect on malignant proliferation of A549 cell in vivo and in vitro.
文摘MicroRNAs(miRNAs),which contains approximately 22 nt,belong to a small endogenous,non-coding regulatory single-stranded RNA molecules.They are posttranscriptional regulators of gene expression and highly conserved in evolution.Many researches show that miRNAs involved in many processes,including tumor formation,cell proliferation and apoptosis and proliferation and metastasis of cancer cells.Among that,the relationship between miRNAs and lung cancer is one of the most focal areas for the researchers,because the abnormal expressions of miRNAs were significantly associated with the occurrence and development of lung cancer.The expression level of different miRNAs in lung cancer cells exist differences,compared with normal lung tissue cells,there are two classes of expression:over-expression level and low expression level.In this review,we focused on studying the mechanism of overexpression miRNAs in lung cancer.
文摘The aim of this work was to study the ontogeny of chondrocyte cell division using embryo, adult and osteoarthritic(OA) cartilage. We searched for mitosis phases and performed a comparative evaluation of mitotic index, basic fibro-blast growth factor b (FGFb), transforming growth factor β1 (TGF-β1) receptors, cyclin dependent kinase (CDK1)and Cyclin-B expression in fetal, neonate, 3, 5, 8 weeks old rats and experimental OA. Our results showed that mitosisphases were observed in all normal cartilage studied, although, we found a decrease in mitotic index in relation to tissuedevelopment. No mitosis was detected in OA cartilage. We also found a statistical significant reduction in cell number inOA cartilage, compared with the normal tissue. Furthermore, FGFb and TGF-β1 receptors diminished in relation totissue development, and were very scarce in experimental OA. Western blot assays showed CDK-1 expression in allcases, including human-OA cartilage. Similar results were observed for Cyclin-B, except for 8 weeks, when it was notexpressed. Our results suggest that cell division seems to be scarce, if not absent within the OA cartilage studied.Nevertheless, the existence of factors essential for cell division leaves open the question concerning chondrocyteproliferation in OA cartilage, which is likely to be present in the early stages of the disease.
基金supported by the National Natural Science Foundation of China (30870535 and 90913017) (B. Zhang) Introduced Innovative R&D Team Program of Guangdong Province (Gene Silencing Technology and Therapeutics)
文摘Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
文摘Objective To explore the mechanism of An-Pressing manipulation in relieving energy crisis in chronic myofascial trigger points(MTrPs)by observing the effects of An-Pressing manipulation on adenosine triphosphate(ATP),adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)/peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α)pathway and mitochondrial ultrastructure of skeletal muscle cells in MTrPs rats.Methods Forty-eight male Sprague-Dawley rats were randomly divided into a blank group,a model group,a lidocaine group,and an An-Pressing manipulation group,with 12 rats in each group.The model group,lidocaine group and An-Pressing manipulation group were used to replicate the MTrPs rat model by blunt shock and centrifugal motion method.After modeling,the An-Pressing manipulation group was subjected to 7 times An-Pressing manipulation,once every other day;the lidocaine group was treated with 3 times of injection of lidocaine at the MTrPs,once every 6 d.The blank group and the model group were fed normally without intervention.After the intervention,local muscle tissue was taken to detect the content of ATP and the expression of AMPK,phosphorylated AMPK(phospho-AMPK),PGC-1α,and glucose transporter 4(GluT4),and the ultrastructure of mitochondria was observed under an electron microscope.Results Compared with the blank group,the ATP content in the model group was decreased(P<0.05),the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were decreased(P<0.05);under the electron microscope,the number of mitochondria decreased,and they were deformed,small in volume,and had deformed cristae.Compared with the model group,the ATP contents in the An-Pressing manipulation group and the lidocaine group were increased(P<0.05),and the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were increased(P<0.05);under the electron microscope,the number of mitochondria increased,the shape and size of the mitochondria were basically normal,and the cristae could be seen.Compared with the lidocaine group,phospho-AMPK and the ratio of phospho-AMPK to AMPK in the An-Pressing manipulation group were increased(P<0.05);under the electron microscope,the numbers of mitochondria were similar,and the shape and size of the mitochondria were basically normal without swelling,and the cristae could be observed.Conclusion An-Pressing manipulation can increase the ATP content in MTrPs tissue,improve the expression levels of PGC-1α and GluT4 proteins and the ratio of phospho-AMPK to AMPK;its mechanism may relate to the activation of AMPK/PGC-1α signaling pathway to promote the repair of mitochondrial damages.
