Drug-coated balloon has been developed as an alternative to drug-eluting stents for in-stent restenosis but the performance of drug infusion balloon in such setting has not been previously described. We present a case...Drug-coated balloon has been developed as an alternative to drug-eluting stents for in-stent restenosis but the performance of drug infusion balloon in such setting has not been previously described. We present a case of particularly aggressive in-stent restenosis after drug eluting stent implantation treated with a new kind of drug infusion balloon developed in order to overcome the impossibility to inflate regular drug-coated balloon for several dilatation.展开更多
Prevention of annual"green tides"caused by blooms of the green macroalga Ulva prolifera,which have occurred in the Yellow Sea since 2007,has received much attention.Increasing evidence indicates that micro-p...Prevention of annual"green tides"caused by blooms of the green macroalga Ulva prolifera,which have occurred in the Yellow Sea since 2007,has received much attention.Increasing evidence indicates that micro-propagules on the rafts used for Porphyra yezoensis aquaculture along the coastline of Jiangsu Province in China,were the primary source of these green tides.In this study,we investigated the effectiveness of a silicone antifouling coating for preventing the adhesion and germination of U.prolifera micro-propagules on bamboo and nylon ropes,which have been used in rafts for P.yezoensis aquaculture.Our results demonstrated that a coating of silicone(Sylgard 184)containing 1.0 wt.%sodium benzoate(NaB)could reduce the adhesion rate to<5%and completely inhibited the growth of U.prolifera propagules on the coated surfaces.Coating bamboo and nylon ropes with NaB-incorporated silicone thus proved a potentially effective method for controlling the initial biomass accumulation of U.prolifera.展开更多
A new technique for mass rearing Clostera anachoreta(C.) was developed through a large numberof experiments, so the problem of mass produce of CaGV was solved. The process of CaGV was discussedin detail.
Objective: This paper applied a transcriptomic approach to investigate the mechanisms of adriamycin(ADR) in treating proliferative vitreoretinopathy(PVR) using ARPE-19 cells. Methods: The growth inhibitory effects of ...Objective: This paper applied a transcriptomic approach to investigate the mechanisms of adriamycin(ADR) in treating proliferative vitreoretinopathy(PVR) using ARPE-19 cells. Methods: The growth inhibitory effects of ADR on ARPE-19 cells were assessed by sulforhodamine B(SRB) assay and propidium iodide(PI) staining using flow cytometry. The differentially expressed genes between ADR-treated ARPE-19 cells and normal ARPE-19 cells and the signaling pathways involved were investigated by microarray analysis. Mitochondrial function was detected by JC-1 staining using flow cytometry and the Bcl-2/Bax protein family. The phosphorylated histone H2 AX(γ-H2 AX), phosphorylated checkpoint kinase 1(p-CHK1), and phosphorylated checkpoint kinase 2(p-CHK2) were assessed to detect DNA damage and repair. Results: ADR could significantly inhibit ARPE-19 cell proliferation and induce caspasedependent apoptosis in vitro. In total, 4479 differentially expressed genes were found, and gene ontology items and the p53 signaling pathway were enriched. A protein–protein interaction analysis indicated that the TP53 protein molecules regulated by ADR were related to DNA damage and oxidative stress. ADR reduced mitochondrial membrane potential and the Bcl-2/Bax ratio. p53-knockdown restored the activation of c-caspase-3 activity induced by ADR by regulating Bax expression, and it inhibited ADR-induced ARPE-19 cell apoptosis. Finally, the levels of the γ-H2 AX, p-CHK1, and p-CHK2 proteins were up-regulated after ADR exposure. Conclusions: The mechanism of ARPE-19 cell death induced by ADR may be caspase-dependent apoptosis, and it may be regulated by the p53-dependent mitochondrial dysfunction, activating the p53 signaling pathway through DNA damage.展开更多
文摘Drug-coated balloon has been developed as an alternative to drug-eluting stents for in-stent restenosis but the performance of drug infusion balloon in such setting has not been previously described. We present a case of particularly aggressive in-stent restenosis after drug eluting stent implantation treated with a new kind of drug infusion balloon developed in order to overcome the impossibility to inflate regular drug-coated balloon for several dilatation.
基金supported by the National Natural Science Foundation of China(Grant No.41276115)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No. XDA11020302)the National Basic Research Program of China(Grant No. 2010CB428706)
文摘Prevention of annual"green tides"caused by blooms of the green macroalga Ulva prolifera,which have occurred in the Yellow Sea since 2007,has received much attention.Increasing evidence indicates that micro-propagules on the rafts used for Porphyra yezoensis aquaculture along the coastline of Jiangsu Province in China,were the primary source of these green tides.In this study,we investigated the effectiveness of a silicone antifouling coating for preventing the adhesion and germination of U.prolifera micro-propagules on bamboo and nylon ropes,which have been used in rafts for P.yezoensis aquaculture.Our results demonstrated that a coating of silicone(Sylgard 184)containing 1.0 wt.%sodium benzoate(NaB)could reduce the adhesion rate to<5%and completely inhibited the growth of U.prolifera propagules on the coated surfaces.Coating bamboo and nylon ropes with NaB-incorporated silicone thus proved a potentially effective method for controlling the initial biomass accumulation of U.prolifera.
文摘A new technique for mass rearing Clostera anachoreta(C.) was developed through a large numberof experiments, so the problem of mass produce of CaGV was solved. The process of CaGV was discussedin detail.
基金Project supported by the Zhejiang Province Key Research and Development Program(No.2015C03042),China
文摘Objective: This paper applied a transcriptomic approach to investigate the mechanisms of adriamycin(ADR) in treating proliferative vitreoretinopathy(PVR) using ARPE-19 cells. Methods: The growth inhibitory effects of ADR on ARPE-19 cells were assessed by sulforhodamine B(SRB) assay and propidium iodide(PI) staining using flow cytometry. The differentially expressed genes between ADR-treated ARPE-19 cells and normal ARPE-19 cells and the signaling pathways involved were investigated by microarray analysis. Mitochondrial function was detected by JC-1 staining using flow cytometry and the Bcl-2/Bax protein family. The phosphorylated histone H2 AX(γ-H2 AX), phosphorylated checkpoint kinase 1(p-CHK1), and phosphorylated checkpoint kinase 2(p-CHK2) were assessed to detect DNA damage and repair. Results: ADR could significantly inhibit ARPE-19 cell proliferation and induce caspasedependent apoptosis in vitro. In total, 4479 differentially expressed genes were found, and gene ontology items and the p53 signaling pathway were enriched. A protein–protein interaction analysis indicated that the TP53 protein molecules regulated by ADR were related to DNA damage and oxidative stress. ADR reduced mitochondrial membrane potential and the Bcl-2/Bax ratio. p53-knockdown restored the activation of c-caspase-3 activity induced by ADR by regulating Bax expression, and it inhibited ADR-induced ARPE-19 cell apoptosis. Finally, the levels of the γ-H2 AX, p-CHK1, and p-CHK2 proteins were up-regulated after ADR exposure. Conclusions: The mechanism of ARPE-19 cell death induced by ADR may be caspase-dependent apoptosis, and it may be regulated by the p53-dependent mitochondrial dysfunction, activating the p53 signaling pathway through DNA damage.
