In order to determine the most optimal host cell line,inoculation dose and the time of harvest,we studied the growth pattern and cytopathic effect (CPE) of the mutants of Pseudorabies virus (PRV) Ea strain,gG -/LacZ +...In order to determine the most optimal host cell line,inoculation dose and the time of harvest,we studied the growth pattern and cytopathic effect (CPE) of the mutants of Pseudorabies virus (PRV) Ea strain,gG -/LacZ +,in different cell lines.A clone virus of gG -/LacZ +,the titer of which could be up to 10 -8.0 /0.1 ml,after purification four times by plaques,was obtained.The clone virus was inoculated into different cell lines,BHK-21,IBRS-2,MDBK and PK-15,respectively.Infected BHK-21 could be induced CPE at 12 hours p.i..However,The CPE of MDBK appeared after 24 hours p.i..In terms of the growth pattern,the titer of IBRS-2 could reach the peak level at 40 hours p.i. and the peak titer was up to 10 -8.0 /0.1 ml,which was maintained until 64 hours p.i..In contrast,the peak of BHK-21 was only 10 -6.5 /0.1 ml during the 96 hours p.i.. We also studied the titer of IBRS-2 when it was inoculated with different dose virus from 0.001 PFU/cell to 100PFU/cell.It was discovered that the titer could reached over 10 -7.5 /0.1 ml when inoculated with 0.1PFU/cell,0.01PFU/cell after 30,48 hours p.i.,respectively.These data indicate that IBRS-2 is the most optimal cell and that the titer could reach the peak when inoculated with 0.01PFU/cell and harvested at 40-50 hours p.i..展开更多
为了探索ND La Sota弱毒株在SPF鸡胚中的增殖规律,保证生产用种毒的质量,我们采用不同的时间段来收获ND La Sota弱毒株感染的胚液,然后测定胚液的EID50.结果发现,SPF鸡胚在接种病毒后85～100h之间出现死亡高峰期,经病毒含量测定,该时段...为了探索ND La Sota弱毒株在SPF鸡胚中的增殖规律,保证生产用种毒的质量,我们采用不同的时间段来收获ND La Sota弱毒株感染的胚液,然后测定胚液的EID50.结果发现,SPF鸡胚在接种病毒后85～100h之间出现死亡高峰期,经病毒含量测定,该时段感染胚液比其他时间死亡的感染胚液病毒含量高出1.5～6倍以上,表明ND La Sota弱毒株的增殖高峰与SPF鸡胚接种后的死亡时间有重要关系.展开更多
文摘In order to determine the most optimal host cell line,inoculation dose and the time of harvest,we studied the growth pattern and cytopathic effect (CPE) of the mutants of Pseudorabies virus (PRV) Ea strain,gG -/LacZ +,in different cell lines.A clone virus of gG -/LacZ +,the titer of which could be up to 10 -8.0 /0.1 ml,after purification four times by plaques,was obtained.The clone virus was inoculated into different cell lines,BHK-21,IBRS-2,MDBK and PK-15,respectively.Infected BHK-21 could be induced CPE at 12 hours p.i..However,The CPE of MDBK appeared after 24 hours p.i..In terms of the growth pattern,the titer of IBRS-2 could reach the peak level at 40 hours p.i. and the peak titer was up to 10 -8.0 /0.1 ml,which was maintained until 64 hours p.i..In contrast,the peak of BHK-21 was only 10 -6.5 /0.1 ml during the 96 hours p.i.. We also studied the titer of IBRS-2 when it was inoculated with different dose virus from 0.001 PFU/cell to 100PFU/cell.It was discovered that the titer could reached over 10 -7.5 /0.1 ml when inoculated with 0.1PFU/cell,0.01PFU/cell after 30,48 hours p.i.,respectively.These data indicate that IBRS-2 is the most optimal cell and that the titer could reach the peak when inoculated with 0.01PFU/cell and harvested at 40-50 hours p.i..
文摘为了探索ND La Sota弱毒株在SPF鸡胚中的增殖规律,保证生产用种毒的质量,我们采用不同的时间段来收获ND La Sota弱毒株感染的胚液,然后测定胚液的EID50.结果发现,SPF鸡胚在接种病毒后85～100h之间出现死亡高峰期,经病毒含量测定,该时段感染胚液比其他时间死亡的感染胚液病毒含量高出1.5～6倍以上,表明ND La Sota弱毒株的增殖高峰与SPF鸡胚接种后的死亡时间有重要关系.
