采用软托盘加诺丹顿增白胶(ROTADENT R Bleaching - Gel)对30例牙色泽异常患者进行漂白治疗,其有效率达100％。其中,对于外源性色斑牙,轻度、中度四环素牙,采用该方法治疗效果更佳。笔者认为在目前众多的治疗牙色泽异常方法中,该方法操...采用软托盘加诺丹顿增白胶(ROTADENT R Bleaching - Gel)对30例牙色泽异常患者进行漂白治疗,其有效率达100％。其中,对于外源性色斑牙,轻度、中度四环素牙,采用该方法治疗效果更佳。笔者认为在目前众多的治疗牙色泽异常方法中,该方法操作简便,应用安全,疗效可靠,建议推广。展开更多
OBJECTIVE There is a close correlation between interleukin-6 （IL-6） and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantit...OBJECTIVE There is a close correlation between interleukin-6 （IL-6） and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantitive RT-PCR and radioimmunoassay were used to examine the effect of bradykinin on the expression of IL-6 in C6 glioma cells and on the level of IL-6 in the culture medium. RESULTS Using semi-quantitive RT-PCR, the expression of IL-6 mRNA was examined in C6 glioma cells at 0, 5, 10,15, 30, 60 rain following addition of 1μmol/L bradykinin. There was no statistical difference in expression of IL-6 mRNA between the treatment and control groups （P〉0.05） and IL-6 was not detected in the cell culture medium. CONCLUSION Within an hour, IL-6 expression in C6 glioma cells is not induced by bradykinin, suggesting that its clinical application may be useful as a potential therapeutic agent for tumors of the central nervous system .展开更多
基金supported by grants from the National Natural Science Foundation of China (No.30670723, No.30600060, No.30400145 and No.30570650)Natu-ral Science Foundation of Liaoning Province (No.20052102).
文摘OBJECTIVE There is a close correlation between interleukin-6 （IL-6） and malignant proliferation of gliomas. We investigated the effect of bradykinin on the expression of IL-6 in C6 glioma cells. METHODS Semi-quantitive RT-PCR and radioimmunoassay were used to examine the effect of bradykinin on the expression of IL-6 in C6 glioma cells and on the level of IL-6 in the culture medium. RESULTS Using semi-quantitive RT-PCR, the expression of IL-6 mRNA was examined in C6 glioma cells at 0, 5, 10,15, 30, 60 rain following addition of 1μmol/L bradykinin. There was no statistical difference in expression of IL-6 mRNA between the treatment and control groups （P〉0.05） and IL-6 was not detected in the cell culture medium. CONCLUSION Within an hour, IL-6 expression in C6 glioma cells is not induced by bradykinin, suggesting that its clinical application may be useful as a potential therapeutic agent for tumors of the central nervous system .
