In this study, Ficus carica L. ‘Masui Dauphine＇ was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Ma...In this study, Ficus carica L. ‘Masui Dauphine＇ was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Masui Dauphine＇. Accord- ing to the results, the most suitable explants for in vitro rapid propagation of ‘Masui Dauphine＇ were axillary buds and the best medium was modified MS ＋ 1.0 mg/L 6-BA ＋ 0. 05 mg/L NAA ＋ 1.0 mg/L GA3 ＋ 20 mg/L sucrose ＋ 7 mg/L agar, pH 5.8. This study provided scientific basis for barge-scale cultivation of‘Masui Dauphine ＇.展开更多
Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowinten...Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of Hep G2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4—0.8 W/cm^2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm^2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering.展开更多
Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize t...Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize the propagation technique of A. mamillata by tissue culture and set up an industrial production system to provide plenty of A. mamillata seedlings for the human demand. The optimal initiation medium for A. mamillata is MS +2.0 mg/L BA +0.1 mg/L NAA +30 g/L sugar, providing76.4% initiation rate. The optimal shoot proliferation medium for A. mamillata is MS+1.0 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 4.56 fold proliferation rate and3.10 cm shoot in height. The optimal shoot elongation medium for A. mamillata is MS+0.5 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 2.77 fold proliferation rate and 4.27 cm shoot in height. The optimal rooting medium for A. mamillata is 1/2MS+0.1 mg/L IBA +15 g/L sugar, providing 99.7% rooting rate, 4.0 roots per individual,7.53 cm root in length and 3.94 cm shoot in height. This provides a reliable mass propagation method for A. mamillata.展开更多
Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately....Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained under the conditions of 32℃, 30 and 240 μmol/（m^2·s） for G. lichenoides, and 24℃, 20 and 200 μmol/（m^2·s） for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions for the highest daily specific growth rates （% increase in wet weight） are determined to be 31.30℃, 32.10, and 287.23 lamol/（m^2·s） for G. lichenoides （16.26%/d）, and 25.38℃, 21.10, and 229.07 lamol/（m^2·s） for G tenuistipitata （14.83%/d）, respectively.展开更多
A marine red tide algae denominated as Heterocapsa circularisquama was cultured under the control factors. And the ecological function of nutrient salts such as nitrogen （mg/L）, phosphorus （rag/L） as well as silic...A marine red tide algae denominated as Heterocapsa circularisquama was cultured under the control factors. And the ecological function of nutrient salts such as nitrogen （mg/L）, phosphorus （rag/L） as well as silicon （rag/L） in different consistency on the population growth of H. circularisquama was investigated. The results showed that overtop or too low consistency of nutrient salts both delayed the population multiplication of the red tide algae, and different nutrient factors as nitrogen, phosphorus and silicon all had significant effects on the population amplification of H.circularisquama （p〈0.01）. The optimal concentration of nitrogen, phosphorus and silicon in the culture medium for the algae propagation and growth were 10 mg/L, 1-5 mg/L and 0 mg/L respectively.展开更多
The California least tern （Sternula antillarum browni） is an endangered species. The two largest colonies of this bird species in the San Francisco Bay, California, USA, are located 23 km apart at Alameda Point and ...The California least tern （Sternula antillarum browni） is an endangered species. The two largest colonies of this bird species in the San Francisco Bay, California, USA, are located 23 km apart at Alameda Point and Hayward Regional Shoreline. The Alameda Point colony, on the former Naval Air Station, Alameda, has grown at an average rate of 9.2% per year since its discovery in 1976 and has a nesting density ranging from 1-153 nests per ha. Long-term breeding success at Alameda has averaged 0.86 fledglings per breeding pair. Least tems began appearing in 2003, at an island in Hayward enhanced with substrate mediums and social attraction devices. Successful breeding attempts have been observed at the Hayward colony since 2006. The Hayward colony has grown at an average rate of 51.5% per year, has a nesting density ranging from 33-333 nests per ha and long-term breeding success average of 0.87 fledglings per breeding pair. Dropped fish have been collected from both colonies, and silversides （family Atherinopsidae） are the dominant prey. Alameda terns forage on a greater variety of species than the Hayward terns. The regression results indicate the importance of Northern anchovy （Engraulis mordax） to the breeding success of the Alameda colony.展开更多
The development, survivorship and reproduction of Bemisia tabaci B bi otype on eggplant at seven constant temperatures (17, 20, 23, 26, 29, 32 and 35℃) were studied. The developmental periods from egg to adult varied...The development, survivorship and reproduction of Bemisia tabaci B bi otype on eggplant at seven constant temperatures (17, 20, 23, 26, 29, 32 and 35℃) were studied. The developmental periods from egg to adult varied from 48.7 days at 17℃ to 13.9 days at 29℃ and the develo pmental threshold estimated for a generation by linear regression was 12.4℃. The optimum temperature for B. tabaci population growth was 26℃, both extremely low (<17℃) and high temperature (>32℃) delayed the de velopment. Survivorships from egg to adult was 67.3 % at 26℃, 27.6 % and 29.0 % at 35℃ and 17℃ resp ectively. The average longevity of females ranged from 39.6 days at 20℃ to 12.8 days at 35℃. Ovipo sition per female varied from 164.8 eggs at 20℃ to 78.5 eggs at 32℃. Both the longevity and oviposition of B. tabaci females at different temperatures were significantly different ( P < 0.05), and the intrinsic rate of natural increase ( r m ) for B. tabaci at 29℃ was the highest.展开更多
文摘In this study, Ficus carica L. ‘Masui Dauphine＇ was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Masui Dauphine＇. Accord- ing to the results, the most suitable explants for in vitro rapid propagation of ‘Masui Dauphine＇ were axillary buds and the best medium was modified MS ＋ 1.0 mg/L 6-BA ＋ 0. 05 mg/L NAA ＋ 1.0 mg/L GA3 ＋ 20 mg/L sucrose ＋ 7 mg/L agar, pH 5.8. This study provided scientific basis for barge-scale cultivation of‘Masui Dauphine ＇.
基金Supported by the Natural Science Foundation of Tianjin(No.12JCYBJC18300)
文摘Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of Hep G2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4—0.8 W/cm^2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm^2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering.
基金Supported by Fujian Modern Agriculture Project:The Innovation and Industrialization Techniques of Dominant Woody Flowering Plants(No.:Min Lin Ji Cai[2012]137)
文摘Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize the propagation technique of A. mamillata by tissue culture and set up an industrial production system to provide plenty of A. mamillata seedlings for the human demand. The optimal initiation medium for A. mamillata is MS +2.0 mg/L BA +0.1 mg/L NAA +30 g/L sugar, providing76.4% initiation rate. The optimal shoot proliferation medium for A. mamillata is MS+1.0 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 4.56 fold proliferation rate and3.10 cm shoot in height. The optimal shoot elongation medium for A. mamillata is MS+0.5 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 2.77 fold proliferation rate and 4.27 cm shoot in height. The optimal rooting medium for A. mamillata is 1/2MS+0.1 mg/L IBA +15 g/L sugar, providing 99.7% rooting rate, 4.0 roots per individual,7.53 cm root in length and 3.94 cm shoot in height. This provides a reliable mass propagation method for A. mamillata.
基金Supported by the 908 Special Program (908-02-04-07)the National Basic Research Program of China (973 Program, No. 2006CB400608)K. C. Wong Magna Fund in Ningbo University
文摘Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained under the conditions of 32℃, 30 and 240 μmol/（m^2·s） for G. lichenoides, and 24℃, 20 and 200 μmol/（m^2·s） for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions for the highest daily specific growth rates （% increase in wet weight） are determined to be 31.30℃, 32.10, and 287.23 lamol/（m^2·s） for G. lichenoides （16.26%/d）, and 25.38℃, 21.10, and 229.07 lamol/（m^2·s） for G tenuistipitata （14.83%/d）, respectively.
文摘A marine red tide algae denominated as Heterocapsa circularisquama was cultured under the control factors. And the ecological function of nutrient salts such as nitrogen （mg/L）, phosphorus （rag/L） as well as silicon （rag/L） in different consistency on the population growth of H. circularisquama was investigated. The results showed that overtop or too low consistency of nutrient salts both delayed the population multiplication of the red tide algae, and different nutrient factors as nitrogen, phosphorus and silicon all had significant effects on the population amplification of H.circularisquama （p〈0.01）. The optimal concentration of nitrogen, phosphorus and silicon in the culture medium for the algae propagation and growth were 10 mg/L, 1-5 mg/L and 0 mg/L respectively.
文摘The California least tern （Sternula antillarum browni） is an endangered species. The two largest colonies of this bird species in the San Francisco Bay, California, USA, are located 23 km apart at Alameda Point and Hayward Regional Shoreline. The Alameda Point colony, on the former Naval Air Station, Alameda, has grown at an average rate of 9.2% per year since its discovery in 1976 and has a nesting density ranging from 1-153 nests per ha. Long-term breeding success at Alameda has averaged 0.86 fledglings per breeding pair. Least tems began appearing in 2003, at an island in Hayward enhanced with substrate mediums and social attraction devices. Successful breeding attempts have been observed at the Hayward colony since 2006. The Hayward colony has grown at an average rate of 51.5% per year, has a nesting density ranging from 33-333 nests per ha and long-term breeding success average of 0.87 fledglings per breeding pair. Dropped fish have been collected from both colonies, and silversides （family Atherinopsidae） are the dominant prey. Alameda terns forage on a greater variety of species than the Hayward terns. The regression results indicate the importance of Northern anchovy （Engraulis mordax） to the breeding success of the Alameda colony.
文摘The development, survivorship and reproduction of Bemisia tabaci B bi otype on eggplant at seven constant temperatures (17, 20, 23, 26, 29, 32 and 35℃) were studied. The developmental periods from egg to adult varied from 48.7 days at 17℃ to 13.9 days at 29℃ and the develo pmental threshold estimated for a generation by linear regression was 12.4℃. The optimum temperature for B. tabaci population growth was 26℃, both extremely low (<17℃) and high temperature (>32℃) delayed the de velopment. Survivorships from egg to adult was 67.3 % at 26℃, 27.6 % and 29.0 % at 35℃ and 17℃ resp ectively. The average longevity of females ranged from 39.6 days at 20℃ to 12.8 days at 35℃. Ovipo sition per female varied from 164.8 eggs at 20℃ to 78.5 eggs at 32℃. Both the longevity and oviposition of B. tabaci females at different temperatures were significantly different ( P < 0.05), and the intrinsic rate of natural increase ( r m ) for B. tabaci at 29℃ was the highest.
