Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of ...Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of human lymphocytes exposed to pesticides （Mancozeb fungicide and Metribuzin herbicide） and on Thl and Th2 cytokine secretion. Their possible protective role on intracellular stress oxidative induced by pesticides was also investigated. Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Histopaque. They were cultured with mitogen concanavalin A （Con A）, Mancozeb and Metribuzin （50 μM） in the presence or the absence of vitamin C （50 μM） or NADH （50 μM）. Proliferation （MTT assay）, IL-2, INFγ and IL-4 （Elisa kits）, oxidative markers （intracellular superoxide anion, hydroperoxides, carbonyl proteins, GSH, catalase and SOD） were determined, The results showed that pesticides were immunosuppressive and decreased cytokine secretion with a shift away from to Thl phenotype. These immunomodulatory properties were accompanied by an increase in lymphocyte intracellular oxidative stress. The presence of vitamin C or NADH, in the medium, exhibited protective effects in human lymphocytes by inhibiting pesticide---induced lymphocyte proliferation suppression, inflammatory status and oxidative stress generation. In conclusion, vitamin C and NADH can result in a safe and effective method to reduce pesticide adverse effects and help to restore immune function.展开更多
In 2012,Zhi-Wei Sun posed many conjectures about the monotonicity of sequences of form {n√zn},where {zn} is a familiar number-theoretic or combinatorial sequence. We show that if the sequence {zn+1/zn}is increasing(r...In 2012,Zhi-Wei Sun posed many conjectures about the monotonicity of sequences of form {n√zn},where {zn} is a familiar number-theoretic or combinatorial sequence. We show that if the sequence {zn+1/zn}is increasing(resp.,decreasing),then the sequence {n√zn} is strictly increasing(resp.,decreasing) subject to a certain initial condition. We also give some sufficient conditions when {zn+1/zn} is increasing,which is equivalent to the log-convexity of {zn}. As consequences,a series of conjectures of Zhi-Wei Sun are verified in a unified approach.展开更多
文摘Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of human lymphocytes exposed to pesticides （Mancozeb fungicide and Metribuzin herbicide） and on Thl and Th2 cytokine secretion. Their possible protective role on intracellular stress oxidative induced by pesticides was also investigated. Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Histopaque. They were cultured with mitogen concanavalin A （Con A）, Mancozeb and Metribuzin （50 μM） in the presence or the absence of vitamin C （50 μM） or NADH （50 μM）. Proliferation （MTT assay）, IL-2, INFγ and IL-4 （Elisa kits）, oxidative markers （intracellular superoxide anion, hydroperoxides, carbonyl proteins, GSH, catalase and SOD） were determined, The results showed that pesticides were immunosuppressive and decreased cytokine secretion with a shift away from to Thl phenotype. These immunomodulatory properties were accompanied by an increase in lymphocyte intracellular oxidative stress. The presence of vitamin C or NADH, in the medium, exhibited protective effects in human lymphocytes by inhibiting pesticide---induced lymphocyte proliferation suppression, inflammatory status and oxidative stress generation. In conclusion, vitamin C and NADH can result in a safe and effective method to reduce pesticide adverse effects and help to restore immune function.
基金supported by National Natural Science Foundation of China(Grant Nos.11071030,11201191 and 11371078)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.20110041110039)+1 种基金National Science Foundation of Jiangsu Higher Education Institutions(GrantNo.12KJB110005)the Priority Academic Program Development of Jiangsu Higher Education Institutions(Grant No.11XLR30)
文摘In 2012,Zhi-Wei Sun posed many conjectures about the monotonicity of sequences of form {n√zn},where {zn} is a familiar number-theoretic or combinatorial sequence. We show that if the sequence {zn+1/zn}is increasing(resp.,decreasing),then the sequence {n√zn} is strictly increasing(resp.,decreasing) subject to a certain initial condition. We also give some sufficient conditions when {zn+1/zn} is increasing,which is equivalent to the log-convexity of {zn}. As consequences,a series of conjectures of Zhi-Wei Sun are verified in a unified approach.
