A study of chitosan blend film as a carrier of corneal endothelial cells

In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film as a carrier of corneal endothelial cell, rabbit corneal endothelial cells cultured in vitro were breeded onto the film. After a cell monolayer formed, the scanning electron micrography was performed. After inplanted into anterior chamber, slit lamp observation, thickness metering, specular microscopy and HE staining were performed at random time after operation to evaluate the biocompatibility. Inflmmnation in anterior, thickness of cornea, cell density, hexagonality and cell size of the surgical cornea were taken as the indexes of biocompatibility. The cultured cells exhibited a confluent monolayer 10 days after incubation, which proved the satisfactory cytocompatibility of this film. Biocompatibility assay results suggested the implantation feasibility of the film as a carder of corneal endothelial cells.

考虑单向预浸料弯曲性能的有限元仿真

热固性树脂基预浸料沿纤维方向具有极高的拉伸模量和较小的弯曲刚度,准确描述预浸料的这种力学特性对于成型过程中褶皱等缺陷的预测与抑制、提升成型过程的有限元仿真精度具有重要意义。本文建立了基于纤维方向准确跟踪并考虑非线性剪切行为的单向预浸料本构模型,并通过共节点壳膜混合单元实现了预浸料高拉伸模量和低弯曲刚度的解耦。同时,以国产AC531/CCF800H单向预浸料为对象,系统测量了未固化预浸料的拉伸模量、剪切模量和弯曲刚度。最后,通过单向预浸料的偏轴拉伸试验和轴向压缩试验分别验证了本文所建立的有限元模型在膜单元和壳单元主导的受力条件下的有效性。