In this paper,we propose a novel spatial data index based on Hadoop:HQ-Tree.In HQ-Tree,we use PR QuadTrec to solve the problem of poor efficiency in parallel processing,which is caused by data insertion order and spac...In this paper,we propose a novel spatial data index based on Hadoop:HQ-Tree.In HQ-Tree,we use PR QuadTrec to solve the problem of poor efficiency in parallel processing,which is caused by data insertion order and space overlapping.For the problem that HDFS cannot support random write,we propose an updating mechanism,called "Copy Write",to support the index update.Additionally,HQ-Tree employs a two-level index caching mechanism to reduce the cost of network transferring and I/O operations.Finally,we develop MapReduce-based algorithms,which are able to significantly enhance the efficiency of index creation and query.Experimental results demonstrate the effectiveness of our methods.展开更多
Objective：To observe the protective effects of erythropoietin （EPO） pretreatment on cardiac myocyte with hypoxia/reoxygenation （H/R） injury and the role of NF-κBin this effects. Methods：After the H/R model of c...Objective：To observe the protective effects of erythropoietin （EPO） pretreatment on cardiac myocyte with hypoxia/reoxygenation （H/R） injury and the role of NF-κBin this effects. Methods：After the H/R model of cardiac myocytes of neonatal rats was established, the cultured cardiac myocytes were divided into 4 groups, including EPO pretreatment group （ EPO 10 U/ml 24 h before H/R）, EPO pretreatment ＋ PDTC group（EPO 10 U/ml and PDTC 5 μg/ml 24 h before H/R）, PDTC group （PDTC 5 μg /ml 24 h before H/R） and eomrolgroup. Before and after the H/R, assay of LDH concentration in the culture medium, the survival rate of the myocytes tested by MTT chromatometry and the apoptosis by flow cytometry were undertaken. Activation of NF-κB was determined by EMSA before and after H/R. Results：EPO pretreatment markedly reduced the LDH concentration in the medium, elevated the survival rate of myocytes and inhibited the apoptosis after H/R. Addition of PDTC during the pretreatment abol- ished the protective effects of EPO pretreatment. NF-κB was markedly activated during EPO pretreatment and PDTCinhibited the activation. However, after H/R, the activity of NF-κB in myocytes with EPO pretreatment was significantly inhibited compared to the other myocytes. Conclusion：NF-κB is significantly activated during EPO pretreatment, but is inhibited after H/R, which is correlated with the protective effects of EPO pretreatment on cardiac myocytes with H/R. This phenomenon can be explained as the negative feedback mechanism of the activation of NF-κB.展开更多
基金This work is supported by the National Natural Science Foundation of China under Grant No.61370091and No.61170200, Jiangsu Province Science and Technology Support Program （industry） Project under Grant No.BE2012179, Program Sponsored for Scientific Innovation Research of College Graduate in Jiangsu Province under Grant No. CXZZ12_0229.
文摘In this paper,we propose a novel spatial data index based on Hadoop:HQ-Tree.In HQ-Tree,we use PR QuadTrec to solve the problem of poor efficiency in parallel processing,which is caused by data insertion order and space overlapping.For the problem that HDFS cannot support random write,we propose an updating mechanism,called "Copy Write",to support the index update.Additionally,HQ-Tree employs a two-level index caching mechanism to reduce the cost of network transferring and I/O operations.Finally,we develop MapReduce-based algorithms,which are able to significantly enhance the efficiency of index creation and query.Experimental results demonstrate the effectiveness of our methods.
文摘Objective：To observe the protective effects of erythropoietin （EPO） pretreatment on cardiac myocyte with hypoxia/reoxygenation （H/R） injury and the role of NF-κBin this effects. Methods：After the H/R model of cardiac myocytes of neonatal rats was established, the cultured cardiac myocytes were divided into 4 groups, including EPO pretreatment group （ EPO 10 U/ml 24 h before H/R）, EPO pretreatment ＋ PDTC group（EPO 10 U/ml and PDTC 5 μg/ml 24 h before H/R）, PDTC group （PDTC 5 μg /ml 24 h before H/R） and eomrolgroup. Before and after the H/R, assay of LDH concentration in the culture medium, the survival rate of the myocytes tested by MTT chromatometry and the apoptosis by flow cytometry were undertaken. Activation of NF-κB was determined by EMSA before and after H/R. Results：EPO pretreatment markedly reduced the LDH concentration in the medium, elevated the survival rate of myocytes and inhibited the apoptosis after H/R. Addition of PDTC during the pretreatment abol- ished the protective effects of EPO pretreatment. NF-κB was markedly activated during EPO pretreatment and PDTCinhibited the activation. However, after H/R, the activity of NF-κB in myocytes with EPO pretreatment was significantly inhibited compared to the other myocytes. Conclusion：NF-κB is significantly activated during EPO pretreatment, but is inhibited after H/R, which is correlated with the protective effects of EPO pretreatment on cardiac myocytes with H/R. This phenomenon can be explained as the negative feedback mechanism of the activation of NF-κB.
