期刊文献+
共找到11篇文章
< 1 >
每页显示 20 50 100
复原胞超光栅中负透射的鲁棒性 被引量:1
1
作者 郭海琴 杜骏杰 《华东师范大学学报(自然科学版)》 CAS CSCD 北大核心 2023年第4期94-100,共7页
基于多重散射理论,研究了复原胞超光栅在发生负向透射,即透射光与入射光在法线同侧时,复原胞的结构对负向透射效率的影响.复原胞由2个不同半径的介质纳米柱组成,它们沿一条线排列起来构成超光栅.结果表明,要实现完美效率,复原胞超光栅... 基于多重散射理论,研究了复原胞超光栅在发生负向透射,即透射光与入射光在法线同侧时,复原胞的结构对负向透射效率的影响.复原胞由2个不同半径的介质纳米柱组成,它们沿一条线排列起来构成超光栅.结果表明,要实现完美效率,复原胞超光栅对小柱子的半径及其在复原胞内的位置并没有非常严格的要求.这意味着复原胞结构对负向透射现象具有很好的鲁棒性,制备和实现高效的复原胞介质超光栅非常可行. 展开更多
关键词 超光栅 负透射 复原胞 鲁棒性
下载PDF
Alterations of red blood cell immunoadherence function in hepatitis B patients
2
作者 孙自勤 王要军 +2 位作者 权启镇 肖瑞明 郭峰 《World Journal of Gastroenterology》 SCIE CAS CSCD 1996年第1期20-21,15,共3页
AIMS To investigate the alterations of RBC immunoadherence function in patients with various hepatitis B. METHODS RBCC3bRR,RBCICRR and serum CIC levels were measured in 42 patients with acute and chronic hepatitis B a... AIMS To investigate the alterations of RBC immunoadherence function in patients with various hepatitis B. METHODS RBCC3bRR,RBCICRR and serum CIC levels were measured in 42 patients with acute and chronic hepatitis B at ac- tive and convalescence stages. RESULTS RBCC3bRRs at the active/acute stage of various hepatitis were decreased.They were 13,54%±5,23% in AH, 7.61%±4.12% in AFH,and 16.18%±6.10% in CH, respectively,all of which were lower than those in normal persons (18.12%±3.91% ).At the quiescent/recovery stage of various hepatitis,the RBCC3bRRs were increased significantly.The changes of RBCICRR and serum CIC level were contrary to those of RBCC3bRR. CONCLUSIONS RBC immunoadherence function is decreased in acute and chronic hepatitis.The decrease is in direct proportion to the severity of the diseases. 展开更多
关键词 hepatitis viral human/immunology erythrocytosis/immunology autigen-antibody complex/blood
下载PDF
A case-control study of the relationship between hepatitis B virus DNA level and risk of hepatocellular carcinoma in Qidong,China 被引量:15
3
作者 Ta o-Tao Liu Ying Fang +5 位作者 Hui Xiong Tao-Yang Chen Zheng-Pin Ni ]ian-Feng Luo Nai-Qing Zhao Xi-Zhong Shen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第19期3059-3063,共5页
AIM:To investigate the role of hepatitis B virus (HBV) replication in the development of hepatocellular carcinoma (HCC), a nested case-control study was performed to study the relationship between HBV DNA level and ri... AIM:To investigate the role of hepatitis B virus (HBV) replication in the development of hepatocellular carcinoma (HCC), a nested case-control study was performed to study the relationship between HBV DNA level and risk of HCC. METHODS:One hundred and seventy cases of HCC and 276 control subjects free of HCC and cirrhosis were selected for this study. Serum HBV DNA level was measured using fluorescein quantitative polymerase chain reaction at study entry and the last visit. RESULTS:In a binary unconditional logistic regression analysis adjusted for age, cigarette smoking, alcohol consumption and family history of chronic liver diseases, the adjusted odds ratios (95% confidence intervals) of HCC in patients with increasing HBV DNA level were 2.834 (1.237-6.492), 48.403 (14.392-162.789), 42.252 (14.784-120.750), and 14.819 (6.992-31.411) for HBV DNA levels ≥ 104 to < 105; ≥ 105 to < 106; ≥ 106 to < 107; ≥ 107 copies/mL, respectively. Forty-six HCC cases were selected to compare the serums viral loads of HBV DNA at study entry with those at the last visit. The HBV DNA levels measured at the two time points did not differ significantly.CONCLUSION:The findings of this study provide strong longitudinal evidence of an increased risk of HCC associated with persistent elevation of serum HBV DNA level in the 104-107 range. 展开更多
关键词 Hepatitis B surface antigen Viral replication Asvmptomatic carriers Viral load
下载PDF
Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA 被引量:2
4
作者 Yun-Qing Yao Ding-Feng Zhang +10 位作者 Ni Tang Ai-Long Huang Xiao-Yi Zou Jiang-Feng Xiao Yun Luo Da-Zhi Zhang Bo Wang Wei-Ping Zhou Hong Ren Qi Liu Shu-Hua Guo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第32期5019-5021,共3页
AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×... AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×1012copies of linear HBV DNA/1×107 PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System.Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group.RESULTS: HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive≥2.1) respectively. HBeAg was negative (<2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected group. Dot blotting revealed that HBV DNA was strongly positive in the transfected group and negative in the control group. Southern blot analysis of intracellular total DNA indicated that there were relaxed circular (rc DNA), covalently closed circular (ccc DNA), and single-stranded (ss DNA) HBV DNA replicative intermediates in the transfected group, there was no integrated HBV DNA in the cellular genome. These parameters were negative in control group.CONCLUSION: Expression and replication of HBV genes can occur in hepatocytes from non-mammalian species.HBV replication has no critical species-specificity, and yet hepatic-specific regulating factors in hepatocytes may be essential for viral replication. 展开更多
关键词 Hepatitis B virus REPLICATION EXPRESSION Primary duck hepatocytes
下载PDF
Herbal compound 861 regulates mRNA expression of collagen synthesis- and degradation-related genes in human hepatic stellate cells 被引量:6
5
作者 Lin Wang Jlan Wang +2 位作者 Xue-Hai Tan Bao-En Wang Pei-Gen Xiao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第11期1790-1794,共5页
AIM: To identify the role of herbal compound 861 (Cpd 861) in the regulation of mRNA expression of collagen synthesis- and degradation-related genes in human hepatic stellate cells (HSCs). METHODS: mRNA levels o... AIM: To identify the role of herbal compound 861 (Cpd 861) in the regulation of mRNA expression of collagen synthesis- and degradation-related genes in human hepatic stellate cells (HSCs). METHODS: mRNA levels of collagen types I and III, matrix metalloproteinase 1 (MMP-1), matrix metalloproteinase 2 (MMP-2), membrane type-1 matrix metalloproteinase (MT1-MMP), tissue inhibitor of metalloproteinase 1 (TIMP-1), and transforming growth factor β1 (TGF-β1) in cultured-activated HSCs treated with Cpd 861 or interferon-γ, (IFN-γ,) were determined by real-time PCR. RESULTS: Both Cpd 861 and IFN-γ reduced the mRNA levels of collagen type Ⅲ, MMP-2 and TGF-β1. Moreover, Cpd 861 significantly enhanced the MMP-1 mRNA levels while down-regulated the TIMP-1 mRNA expression, increasing the ratio of MMP-1 to TIMP-1 to (6.3 + 0.3)- fold compared to the control group. CONCLUSION: The anti-fibrosis function of Cpd 861 may be mediated by both decreased interstitial collagen sythesis by inhibiting the transcription of collagen type Ⅲ and TGF-β1 and increased degradation of these collagens by up-regulating MMP-1 and down-regulating TIMP-1 mRNA levels. 展开更多
关键词 Herbal Compound 861 Human hepatic stellate cells Collagen synthesis and degration Collagen type Matrix metalloproteinase 1 Tissue inhibitor of metalloproteinase 1
下载PDF
Trichosanthin inhibits T cell activation by interfering with the recruitment of ZAP-70 to CD3 ζchain 被引量:3
6
作者 HONG JIAN SAI LI FU +2 位作者 ZHONG YI SHEN PEI HUA LU KUANG YEN CHOU (Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai China) 《Cell Research》 SCIE CAS CSCD 1998年第1期33-39,共7页
Plant protein Trichosanthin (Tk) has been shown in our previous experiments to suppress antigenic response of T cells. Here we explored its inhibitory mechanisms on the proliferation of human Jurkat leukemia T cell tr... Plant protein Trichosanthin (Tk) has been shown in our previous experiments to suppress antigenic response of T cells. Here we explored its inhibitory mechanisms on the proliferation of human Jurkat leukemia T cell triggered by anti-CD3 McAb. By examination of tyrosine phosphorylation of cell lysate, we were able to show that Tk could interfere with the PTK-related activity in the TCR/CD3initiated signal transduction in addition to blocking the phosphorylation of PKC. As shown in our experiment,the expression intensity of ZAP-70, a kind of protein tyrosine kinase, was not changed but its phosphorylation could be inhibited. When physical link between CD3(chain and ZAP-70 was further examined by using coimmunoprecipitation after pluse-treatment of the cell line with Tk, the anti-CD3 McAb-induced recruitment of ZAP70 to CD3 ζ chain was observed to be blocked in some extent. This may account for, at least in part, how Trichosanthin was able to inhibit the TCR-triggered T cell proliferation. 展开更多
关键词 TRICHOSANTHIN TCR signaling PTK tyrosine phosphorylation
下载PDF
Treatment of chronic proliferative cholangitis with c-myc shRNA 被引量:6
7
作者 Fu-Yu Li Nan-Sheng Cheng +4 位作者 Jing-Qiu Cheng Hui Mao Li-Sheng Jiang Ning Li Sheng He 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第1期95-101,共7页
AIM: To investigate the feasibility and effectiveness of c-myc shRNA in inhibiting the hyperplastic behavior and lithogenic potentiality of chronic proliferative cholangitis (CPC), in order to prevent stone recurre... AIM: To investigate the feasibility and effectiveness of c-myc shRNA in inhibiting the hyperplastic behavior and lithogenic potentiality of chronic proliferative cholangitis (CPC), in order to prevent stone recurrence and biliary restenosis. METHODS: An animal model of CPC was established by giving intralumenally 0.5 mL of c-myc shRNA. Then, the effects of c-myc shRNA on hyperplastic behavior and lithogenic potentiality of CPC were evaluated by histological observation, immunohistochemistry, real- time PCR and Western blotting for c-myc, proliferating cell nuclear antigen (PCNA), procollagen m, mucin 5AC, enzymatic histochemistry for 13-glucuronidase, and biochemistry for hydroxyproline in the diseased bile duct. RESULTS: Treatment with c-myc shRNA efficiently suppressed the hyperplasia of biliary epithelium, submucosal gland, and collagen fiber by inhibiting mRNA and protein expression of c-myc. More importantly, it decreased the lithogenic potentiality of CPC by inhibiting the expression of mucin 5AC and the secretion of endogenous 13-glucuronidase. Further investigation indicated that c-myc shRNA-3 had a better inhibitory effect on CPC. CONCLUSION: Treatment with c-myc shRNA-3 can control CPC and reduce the lithogenic potentiality of CPC. 展开更多
关键词 Chronic proliferative cholangitis Hepatoli-thiasis RECURRENCE C-MYC PREVENTION
下载PDF
Gene-Ontology Analysis on the Differentially Expressed Genes in Maize (Zea mays L.) Ear Rot 被引量:2
8
作者 Guang-Sheng Yuan Jian Gao Zhi-Ming Zhang Juan Du Gui-Qing Mu Guang-Tang Pan 《Journal of Life Sciences》 2013年第3期219-226,共8页
To better know FM (Fusarium moniliforme) induced genes in maize ear rot, GO (gene ontology) method was performed to analyze detail physiological functions in the defensive response after pathogen infection. This g... To better know FM (Fusarium moniliforme) induced genes in maize ear rot, GO (gene ontology) method was performed to analyze detail physiological functions in the defensive response after pathogen infection. This gene annotation system was widely used to investigate large numbers of genes involving in real active role or regulator in cell response. First of all, differentially expressed genes were isolated by using genechip platform at 96 h post-inoculation with FM in maize inbred Bt-1. In total, 482 differentially expressed unique genes were screened out in inbred Bt-1 when compared to mock-inoculated bract tissues. Then, each gene was annotated to define functional class by GO method. Finally, these large FM-responsive genes with significant differentially change were sorted into cellular component, molecular function and biological process with complicated network by molecular annotation system. The demonstrated information in the GO analysis could provide another view for understanding the molecular mechanism and indicate a deeply complicated network with gene function underlying disease development in the host tissue. The findings in this study provide important bases to probe the molecular processes, the alteration of metabolism and the immune mechanism upon the FM infection in maize. 展开更多
关键词 Ear rot GENECHIP Fusarium moniliforme gene ontology Zea mays.
下载PDF
Rebamipide promotes healing of colonic ulceration through enhanced epithelial restitution
9
作者 Tomohisa Takagi Yuji Naito +9 位作者 Kazuhiko Uchiyama Toshimitsu Okuda Katsura Mizushima Takahiro Suzuki Osamu Handa Takeshi Ishikawa Nobuaki Yagi Satoshi Kokura Hiroshi Ichikawa Toshikazu Yoshikawa 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第33期3802-3809,共8页
AIM:To investigate the efficacy of rebamipide in a rat model of colitis and restitution of intestinal epithelial cells in vitro.METHODS:Acute colitis was induced with trinitrobenzene sulfonic acid(TNBS)in male Wistar ... AIM:To investigate the efficacy of rebamipide in a rat model of colitis and restitution of intestinal epithelial cells in vitro.METHODS:Acute colitis was induced with trinitrobenzene sulfonic acid(TNBS)in male Wistar rats.Rats received intrarectal rebamipide treatment daily starting on day 7 and were sacrificed on day 14 after TNBS administration.The distal colon was removed to evaluate the various parameters of inflammation.Moreover,wound healing assays were used to determine the enhanced restitution of rat intestinal epithelial(RIE)cells treated with rebamipide.RESULTS:Intracolonic administration of rebamipide accelerated TNBSinduced ulcer healing.Increases in the wet weight of the colon after TNBS administration were significantly inhibited by rebamipide.The wound assay revealed that rebamipide enhanced the migration of RIE cells through phosphorylation of extracellular signalregulated kinase(ERK)and activation of Rho kinase.CONCLUSION:Rebamipide enema healed intestinal injury by enhancing restitution of RIE cells,via ERK activation.Rebamipide might be a novel therapeutic approach for inflammatory bowel disease. 展开更多
关键词 REBAMIPIDE Experimental colitis Intestinal epithelial cells Extracellular signalregulated kinase Rho kinase
下载PDF
Epidemiology and gene markers of ulcerative colitis in the Chinese 被引量:36
10
作者 Jun Yun Chang-Tai Xu Bo-Rong Pan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第7期788-803,共16页
Inflammatory bowel disease (IBD) includes two similar yet distinct conditions called ulcerative colitis (UC) and Crohn's disease (CD). These diseases affect the digestive system and cause the inflammation of in... Inflammatory bowel disease (IBD) includes two similar yet distinct conditions called ulcerative colitis (UC) and Crohn's disease (CD). These diseases affect the digestive system and cause the inflammation of intestinal tissue, form sores and bleed easily. Most children with IBD are diagnosed in late childhood and adolescence. However, both UC and CD have been reported as early as in infancy. Most information pertaining to the epidemiology of IBD is based upon adult studies. Symptoms include abdominal pain, cramping, fatigue and diarrhea. Genetic factors play a significant role in determining IBD susceptibility. Epidemiological data support a genetic contribution to the pathogenesis of IBD. Recently, numerous new genes have been identified as being involved in the genetic susceptibility to IBD: TNF- 308A, CARD15 (NOD2), MIF-173, N-acetyltransferase 2 (NAT2), NKG2D (natural killer cell 2D), STAT6 (signal transducer and activator of transcription 6), CTLA-4 (cytotoxic T lymphocyte antigen-4), MICA-MICB (major histocompatibility complex A and B), HLA-DRB1, HLA class-Ⅱ, IL-18, IL-4, MICA-A5, CD14, TI R4, Fas-670, p53 and NF-kB. The characterization of these novel genes has the potential to identify therapeutic agents and aid clinical assessment of phenotype and prognosis in patients with IBD (UC and CD). 展开更多
关键词 GENETIC Inflammatory bowel disease Ulcerative colitis Crohn's disease EPIDEMIOLOGY SUSCEPTIBILITY GENE
下载PDF
Mineralization regulation and biological influence of bioactive glass-collagen-phosphatidylserine composite scaffolds 被引量:1
11
作者 YANG ChunRong WANG YingJun CHEN XiaoFeng 《Science China(Life Sciences)》 SCIE CAS 2012年第3期236-240,共5页
Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies.In the present study,novel biomimetic composite scaffolds,with similar properties to natural bone,were pre... Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies.In the present study,novel biomimetic composite scaffolds,with similar properties to natural bone,were prepared,blended and cross-linked with bioactive glass,type I collagen and phosphatidylserine.When exposed to cell culture solution in the absence of a cellular source,the composite scaffolds form crystals with octahedral structure.These crystals are similar to the products derived from MC3T3-E1 cell mineralization within the composite scaffolds,with respect to both composition and morphology.Furthermore,crystals with octahedral structure were observed to develop into plate-like hydroxyapatite.The bio-mineralization behavior of the composite scaffolds is likely influenced by inorganic components.Finally,a rabbit tibia defect model shows that the highly bioactive properties of the investigated composites result in excellent bone repair. 展开更多
关键词 composite SCAFFOLD cell bio-mineralization REGULATION
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部