目的:探讨关节镜下改良Brostrom-Mason-Allen术治疗复发性踝关节外侧副韧带损伤的临床疗效。方法:回顾性分析我院2018年1月~2020年1月接受关节镜下改良Brostrom-Mason-Allen术治疗的30例复发性踝关节外侧副韧带损伤患者,男18例、女12例...目的:探讨关节镜下改良Brostrom-Mason-Allen术治疗复发性踝关节外侧副韧带损伤的临床疗效。方法:回顾性分析我院2018年1月~2020年1月接受关节镜下改良Brostrom-Mason-Allen术治疗的30例复发性踝关节外侧副韧带损伤患者,男18例、女12例,左侧13例、右侧17例。通过比较患者术前及术后1周、3月及末次随访时疼痛视觉模拟评分(VAS),术前及术后2月、6月、1年及末次随访时美国足踝外科协会(AOFAS)评分以及末次随访使用关节韧带数字体查仪(Ligs)测量患者双侧踝关节前抽屉实验位移以评价手术疗效。结果:术后患者切口均一期愈合,未出现感染、植入反应、肌腱损伤、神经损伤或血管损伤等并发症。患者随访时间24.3个月(20~36个月)。患者术后VAS评分、AOFAS评分较术前明显改善,且差异具有统计学意义(P<0.001)。患者末次随访时踝关节前抽屉实验健侧与患侧位移(11.34±1.35 mm vs. 11.36±1.18 mm),差异无统计学意义(t=-0.08,P>0.05)。结论:关节镜下改良Brostrom-Mason-Allen术治疗复发性踝关节外侧副韧带损伤,手术创伤小,近期临床疗效好且安全可靠,术后踝关节功能恢复满意。展开更多
AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×...AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×1012copies of linear HBV DNA/1×107 PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System.Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group.RESULTS: HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive≥2.1) respectively. HBeAg was negative (＜2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected group. Dot blotting revealed that HBV DNA was strongly positive in the transfected group and negative in the control group. Southern blot analysis of intracellular total DNA indicated that there were relaxed circular (rc DNA), covalently closed circular (ccc DNA), and single-stranded (ss DNA) HBV DNA replicative intermediates in the transfected group, there was no integrated HBV DNA in the cellular genome. These parameters were negative in control group.CONCLUSION: Expression and replication of HBV genes can occur in hepatocytes from non-mammalian species.HBV replication has no critical species-specificity, and yet hepatic-specific regulating factors in hepatocytes may be essential for viral replication.展开更多
Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and ...Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and HSR injuries. The expressions of TNF-α, IL-6, G-CSF and ICAM-1 mRNAs in the liver were examined by RT-PCR, and their serum levels were measured by ELISA. The liver inflammatory infiltration and liver injury in both Egr-1 WT and KO mice following HS/R were evaluated by liver MPO content, serum ALT level and histological examination. Results: Egr-1 inhibition resulted in less mRNA expression of TNF-α, IL-6 , G-CSF and ICAM-1 in the liver, and lower serum levels of TNF-α, IL-6, G-CSF and ICAM-1 antigens in Egr-1 KO mice following HS/R. The liver inflammatory infiltration and liver injury were less severe in Egr-1 KO mice following HS/R, as evidenced by lower serum ALT level, lower hepatic MPO content and histological manifestations. Conclusion: Our data suggest that transcription factor Egr-1 is involved in regulating the expression of inflammatory response genes and plays a role in liver injury following HS/R.展开更多
In order to get a deep understanding of composite failure mechanisms, the new advanced signal processing methodologies are established for the analysis of the large number of acoustic emission (AE) data obtained from ...In order to get a deep understanding of composite failure mechanisms, the new advanced signal processing methodologies are established for the analysis of the large number of acoustic emission (AE) data obtained from the quasi-static tension test of carbon fiber twill weave composite. For this purpose, AE signals have been collected and post-processed for tension test, and are analyzed with three signal processing methods: Empirical Mode Decomposition (EMD), Hilbert-Huang Transform (HHT) and modified energy entropy algorithm. AE signals can be decomposed into a set of Intrinsic Mode Functions (IMF) components, results from this study reveal that the peak frequency of IMF components based on Fast Fourier Transform (FFT) corresponds to different damage mechanisms of composite. HHT of AE signals can clearly express the frequency distribution of IMF component in time-scale in different damage stages, and can calculate accurate instantaneous frequency for damage modes recognition. The energy entropy based on EMD is introduced to act as a new relevant descriptor of composite damage modes in order to improve the characterization and the discrimination of the damage mechanisms.展开更多
文摘目的:探讨关节镜下改良Brostrom-Mason-Allen术治疗复发性踝关节外侧副韧带损伤的临床疗效。方法:回顾性分析我院2018年1月~2020年1月接受关节镜下改良Brostrom-Mason-Allen术治疗的30例复发性踝关节外侧副韧带损伤患者,男18例、女12例,左侧13例、右侧17例。通过比较患者术前及术后1周、3月及末次随访时疼痛视觉模拟评分(VAS),术前及术后2月、6月、1年及末次随访时美国足踝外科协会(AOFAS)评分以及末次随访使用关节韧带数字体查仪(Ligs)测量患者双侧踝关节前抽屉实验位移以评价手术疗效。结果:术后患者切口均一期愈合,未出现感染、植入反应、肌腱损伤、神经损伤或血管损伤等并发症。患者随访时间24.3个月(20~36个月)。患者术后VAS评分、AOFAS评分较术前明显改善,且差异具有统计学意义(P<0.001)。患者末次随访时踝关节前抽屉实验健侧与患侧位移(11.34±1.35 mm vs. 11.36±1.18 mm),差异无统计学意义(t=-0.08,P>0.05)。结论:关节镜下改良Brostrom-Mason-Allen术治疗复发性踝关节外侧副韧带损伤,手术创伤小,近期临床疗效好且安全可靠,术后踝关节功能恢复满意。
基金Supported by the National Natural Science Foundation of China,No. 39670340the Applied Basic Research Programs of ScienceTechnology Commission Foundation of Chongqing, No.20021889
文摘AIM: To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).METHODS: Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19×1012copies of linear HBV DNA/1×107 PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System.Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group.RESULTS: HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive≥2.1) respectively. HBeAg was negative (＜2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected group. Dot blotting revealed that HBV DNA was strongly positive in the transfected group and negative in the control group. Southern blot analysis of intracellular total DNA indicated that there were relaxed circular (rc DNA), covalently closed circular (ccc DNA), and single-stranded (ss DNA) HBV DNA replicative intermediates in the transfected group, there was no integrated HBV DNA in the cellular genome. These parameters were negative in control group.CONCLUSION: Expression and replication of HBV genes can occur in hepatocytes from non-mammalian species.HBV replication has no critical species-specificity, and yet hepatic-specific regulating factors in hepatocytes may be essential for viral replication.
文摘Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and HSR injuries. The expressions of TNF-α, IL-6, G-CSF and ICAM-1 mRNAs in the liver were examined by RT-PCR, and their serum levels were measured by ELISA. The liver inflammatory infiltration and liver injury in both Egr-1 WT and KO mice following HS/R were evaluated by liver MPO content, serum ALT level and histological examination. Results: Egr-1 inhibition resulted in less mRNA expression of TNF-α, IL-6 , G-CSF and ICAM-1 in the liver, and lower serum levels of TNF-α, IL-6, G-CSF and ICAM-1 antigens in Egr-1 KO mice following HS/R. The liver inflammatory infiltration and liver injury were less severe in Egr-1 KO mice following HS/R, as evidenced by lower serum ALT level, lower hepatic MPO content and histological manifestations. Conclusion: Our data suggest that transcription factor Egr-1 is involved in regulating the expression of inflammatory response genes and plays a role in liver injury following HS/R.
基金supported by the National Natural Science Foundation of China (Grand No.51275221)the Natural Science Foundation of Jiangsu Province, China (Grand No. BK2011261)
文摘In order to get a deep understanding of composite failure mechanisms, the new advanced signal processing methodologies are established for the analysis of the large number of acoustic emission (AE) data obtained from the quasi-static tension test of carbon fiber twill weave composite. For this purpose, AE signals have been collected and post-processed for tension test, and are analyzed with three signal processing methods: Empirical Mode Decomposition (EMD), Hilbert-Huang Transform (HHT) and modified energy entropy algorithm. AE signals can be decomposed into a set of Intrinsic Mode Functions (IMF) components, results from this study reveal that the peak frequency of IMF components based on Fast Fourier Transform (FFT) corresponds to different damage mechanisms of composite. HHT of AE signals can clearly express the frequency distribution of IMF component in time-scale in different damage stages, and can calculate accurate instantaneous frequency for damage modes recognition. The energy entropy based on EMD is introduced to act as a new relevant descriptor of composite damage modes in order to improve the characterization and the discrimination of the damage mechanisms.
