利用花叶1号×紫茎1号杂交后代衍生的208个F2家系组建群体,构建含有95个SSR标记位点的遗传连锁图谱,该图谱包含11个连锁群,全长1457.47 c M,标记平均间距为15.34 c M。利用复合区间作图法,对株高、幼茎色、主茎色、生长习性、结荚...利用花叶1号×紫茎1号杂交后代衍生的208个F2家系组建群体,构建含有95个SSR标记位点的遗传连锁图谱,该图谱包含11个连锁群,全长1457.47 c M,标记平均间距为15.34 c M。利用复合区间作图法,对株高、幼茎色、主茎色、生长习性、结荚习性、复叶叶形和成熟叶色等农艺性状进行QTL分析,分别检测到与株高、幼茎色、主茎色、复叶叶形有关的QTL各1个,贡献率在8.49%~66.64%之间;与结荚习性有关的QTL3个,贡献率在60.32%~80.36%之间;与成熟叶色有关QTL 4个,贡献率在69.06%~87.35%之间;与生长习性有关的QTL数量最多,共26个,贡献率在58.32%~99.51%之间。上述QTL主要分布在LG1、LG2、LG4、LG8和LG10连锁群,其中LG1最少,仅检测到生长习性的1个QTL,LG4最多,包含了幼茎色、主茎色、结荚习性、生长习性、复叶叶形、成熟期叶色6个农艺性状的15个QTL;这些QTL既可以应用于绿豆育种的分子标记辅助选择,也对深入研究这些性状的遗传奠定了基础。展开更多
Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carr...Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.展开更多
Breeding rice with high water use efficiency (WUE) can ameliorate water shortage through water-saving irrigation.However,WUE is a complex quantitative trait and very few studies have been conducted to measure WUE dire...Breeding rice with high water use efficiency (WUE) can ameliorate water shortage through water-saving irrigation.However,WUE is a complex quantitative trait and very few studies have been conducted to measure WUE directly.In this study,a recombined inbred line population derived from a cross between an indica lowland rice and upland japonica rice was used to dissect the genetic control of WUE by fine-monitored water supply experiments.Quantitative trait loci (QTL) were scanned for 10 traits including heading date (HD),water-consumption per day (water/d),shoot weight gain per day (shootw/d),root weight gain per day (rootw/d),kernel weight gain per day (kernelw/d),average WUE at whole plant level (WUEwhole/d),average WUE for up-ground biomass (WUEup/d),average WUE for grain yield (WUEyield/d),average economic index (econindex/d),and average root/shoot ratio per day (ratio/d).The results show that most of the traits were significantly correlated to each other.Twenty-four QTL (LOD ≥ 2.0) were detected for econindex,econindex/d,WUEyield,WUEyield/d,WUEup,WUEup/d,WUEwhole,WUEwhole/d,kernelw,kernelw/d,rootw,and water/d by composite interval mapping.These QTLs are located on chromosomes 1,2,4,6,7,8,and 12.Individual QTLs accounted for 4.97%-10.78% of the phenotypic variation explained.Some of these QTLs overlapped with previously reported drought resistance QTLs detected in this population.These results provide useful information for further dissection of the genetic basis and marker-assisted selection of WUE in rice.展开更多
文摘利用花叶1号×紫茎1号杂交后代衍生的208个F2家系组建群体,构建含有95个SSR标记位点的遗传连锁图谱,该图谱包含11个连锁群,全长1457.47 c M,标记平均间距为15.34 c M。利用复合区间作图法,对株高、幼茎色、主茎色、生长习性、结荚习性、复叶叶形和成熟叶色等农艺性状进行QTL分析,分别检测到与株高、幼茎色、主茎色、复叶叶形有关的QTL各1个,贡献率在8.49%~66.64%之间;与结荚习性有关的QTL3个,贡献率在60.32%~80.36%之间;与成熟叶色有关QTL 4个,贡献率在69.06%~87.35%之间;与生长习性有关的QTL数量最多,共26个,贡献率在58.32%~99.51%之间。上述QTL主要分布在LG1、LG2、LG4、LG8和LG10连锁群,其中LG1最少,仅检测到生长习性的1个QTL,LG4最多,包含了幼茎色、主茎色、结荚习性、生长习性、复叶叶形、成熟期叶色6个农艺性状的15个QTL;这些QTL既可以应用于绿豆育种的分子标记辅助选择,也对深入研究这些性状的遗传奠定了基础。
基金Supported by the National Basic Research Program of China (Grant No. 2010CB126000)National High Technology Research and Development Program of China (Grant No. 2006011001044)the National Natural Science Foundation of China (Grant Nos. 30671322 and 30871561)
文摘Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.
基金supported by the National Natural Science Foundation of China (30725021 and 30921091)
文摘Breeding rice with high water use efficiency (WUE) can ameliorate water shortage through water-saving irrigation.However,WUE is a complex quantitative trait and very few studies have been conducted to measure WUE directly.In this study,a recombined inbred line population derived from a cross between an indica lowland rice and upland japonica rice was used to dissect the genetic control of WUE by fine-monitored water supply experiments.Quantitative trait loci (QTL) were scanned for 10 traits including heading date (HD),water-consumption per day (water/d),shoot weight gain per day (shootw/d),root weight gain per day (rootw/d),kernel weight gain per day (kernelw/d),average WUE at whole plant level (WUEwhole/d),average WUE for up-ground biomass (WUEup/d),average WUE for grain yield (WUEyield/d),average economic index (econindex/d),and average root/shoot ratio per day (ratio/d).The results show that most of the traits were significantly correlated to each other.Twenty-four QTL (LOD ≥ 2.0) were detected for econindex,econindex/d,WUEyield,WUEyield/d,WUEup,WUEup/d,WUEwhole,WUEwhole/d,kernelw,kernelw/d,rootw,and water/d by composite interval mapping.These QTLs are located on chromosomes 1,2,4,6,7,8,and 12.Individual QTLs accounted for 4.97%-10.78% of the phenotypic variation explained.Some of these QTLs overlapped with previously reported drought resistance QTLs detected in this population.These results provide useful information for further dissection of the genetic basis and marker-assisted selection of WUE in rice.
