AIM： To prepare a complex of hyaluronic acid （HA） and phospholipids （PL）, and study the improvement effect of PL on the oral absorption of HA. METHODS： The complex of HA-PL （named Haplex） was prepared by film ...AIM： To prepare a complex of hyaluronic acid （HA） and phospholipids （PL）, and study the improvement effect of PL on the oral absorption of HA. METHODS： The complex of HA-PL （named Haplex） was prepared by film dispersion and sonication method, its physico-chemical properties were identified by infrared spectra and differential scanning calorimetry （DSC）. The oral absorption of Haplex was studied. Thirty-two healthy rats were divided into 4 groups randomly： （1） a normal saline （NS） control group; （2） an HA group; （3） a mixture group and （4） a Haplex group. After intragastric administration, the concentration of HA in serum was determined. RESULTS： The physico-chemical properties of Haplex were different from HA or PL or their mixture. After Haplex was administered to rats orally, the serum concentration of HA was increased when compared with the mixture or HA control groups from 4 h to 10 h （P 〈 0.05）. The AAUCo-12, of Haplex was also greater than that of the other three groups （P 〈 0.05）. CONCLUSION： The method of film dispersion and sonication can prepare HA and PL complex, and PL can enhance the oral absorption of exogenous HA.展开更多
Objective ：To explore the effects of motilin in the hippocampus on the interdigestive migrating motor complex （MMC） in rats. Methods： Adult SD rats of either sex were used; 0.5 μl motilin （0. 74 retool/L） was i...Objective ：To explore the effects of motilin in the hippocampus on the interdigestive migrating motor complex （MMC） in rats. Methods： Adult SD rats of either sex were used; 0.5 μl motilin （0. 74 retool/L） was injected into the guide cannula which was stereotaxically implanted into the hippocampus previously. Then the MMC was recorded by a RM6240B multilead physiological recording system. Resuits： （1）MMC characteristics of normal rats＇ duodenum： the frequency of phase Ⅲ was （18. 1±0. 4） bursts/min; the amplitude of phase Ⅲ was （260.5±42.3）μV; the duration of phase Ⅲ was （354.1±21.6） s; MMC cycle duration was （690.2±58.7）s. （2） After motilin was injected into the hippocampus, the duodenal MMC cycle duration was decreased significantly. However, the amplitude of phase Ⅲ and the frequency of phase Ⅲ were increased. But there were no effects on the duration of phase Ⅲ . Frequency of phase Ⅲ percentage change was much more than amplitude of phase Ⅲ percentage change （57.2±2.8 vs 39.3±5. 2）. （3） Effects of motilin in the hippocampus on MMC were completely abolished by subdiaphragmal vagotomy. （4） Effects of motilin in the hippocampus on MMC were unaffected by intravenously injected atropine, phentolamine or propranolol. （5） The anti-motilin serum partly abolished the effects of motilin in the hippocampus on MMC. Conclusion： Motilin in the hippocampus has effects on the duodenal MMC cycle duration, the amplitude of phase Ⅲ and the frequency of phase Ⅲ. Motilin in the hippocampus plays an important role in duodenal MMC.展开更多
AIM:To investigate the efficacy of rebamipide in a rat model of colitis and restitution of intestinal epithelial cells in vitro.METHODS:Acute colitis was induced with trinitrobenzene sulfonic acid(TNBS)in male Wistar ...AIM:To investigate the efficacy of rebamipide in a rat model of colitis and restitution of intestinal epithelial cells in vitro.METHODS:Acute colitis was induced with trinitrobenzene sulfonic acid(TNBS)in male Wistar rats.Rats received intrarectal rebamipide treatment daily starting on day 7 and were sacrificed on day 14 after TNBS administration.The distal colon was removed to evaluate the various parameters of inflammation.Moreover,wound healing assays were used to determine the enhanced restitution of rat intestinal epithelial(RIE)cells treated with rebamipide.RESULTS:Intracolonic administration of rebamipide accelerated TNBSinduced ulcer healing.Increases in the wet weight of the colon after TNBS administration were significantly inhibited by rebamipide.The wound assay revealed that rebamipide enhanced the migration of RIE cells through phosphorylation of extracellular signalregulated kinase(ERK)and activation of Rho kinase.CONCLUSION:Rebamipide enema healed intestinal injury by enhancing restitution of RIE cells,via ERK activation.Rebamipide might be a novel therapeutic approach for inflammatory bowel disease.展开更多
基金the Natural Science Foundation of Shandong Province, China. No. Y2005C31
文摘AIM： To prepare a complex of hyaluronic acid （HA） and phospholipids （PL）, and study the improvement effect of PL on the oral absorption of HA. METHODS： The complex of HA-PL （named Haplex） was prepared by film dispersion and sonication method, its physico-chemical properties were identified by infrared spectra and differential scanning calorimetry （DSC）. The oral absorption of Haplex was studied. Thirty-two healthy rats were divided into 4 groups randomly： （1） a normal saline （NS） control group; （2） an HA group; （3） a mixture group and （4） a Haplex group. After intragastric administration, the concentration of HA in serum was determined. RESULTS： The physico-chemical properties of Haplex were different from HA or PL or their mixture. After Haplex was administered to rats orally, the serum concentration of HA was increased when compared with the mixture or HA control groups from 4 h to 10 h （P 〈 0.05）. The AAUCo-12, of Haplex was also greater than that of the other three groups （P 〈 0.05）. CONCLUSION： The method of film dispersion and sonication can prepare HA and PL complex, and PL can enhance the oral absorption of exogenous HA.
文摘Objective ：To explore the effects of motilin in the hippocampus on the interdigestive migrating motor complex （MMC） in rats. Methods： Adult SD rats of either sex were used; 0.5 μl motilin （0. 74 retool/L） was injected into the guide cannula which was stereotaxically implanted into the hippocampus previously. Then the MMC was recorded by a RM6240B multilead physiological recording system. Resuits： （1）MMC characteristics of normal rats＇ duodenum： the frequency of phase Ⅲ was （18. 1±0. 4） bursts/min; the amplitude of phase Ⅲ was （260.5±42.3）μV; the duration of phase Ⅲ was （354.1±21.6） s; MMC cycle duration was （690.2±58.7）s. （2） After motilin was injected into the hippocampus, the duodenal MMC cycle duration was decreased significantly. However, the amplitude of phase Ⅲ and the frequency of phase Ⅲ were increased. But there were no effects on the duration of phase Ⅲ . Frequency of phase Ⅲ percentage change was much more than amplitude of phase Ⅲ percentage change （57.2±2.8 vs 39.3±5. 2）. （3） Effects of motilin in the hippocampus on MMC were completely abolished by subdiaphragmal vagotomy. （4） Effects of motilin in the hippocampus on MMC were unaffected by intravenously injected atropine, phentolamine or propranolol. （5） The anti-motilin serum partly abolished the effects of motilin in the hippocampus on MMC. Conclusion： Motilin in the hippocampus has effects on the duodenal MMC cycle duration, the amplitude of phase Ⅲ and the frequency of phase Ⅲ. Motilin in the hippocampus plays an important role in duodenal MMC.
基金Supported by A GrantinAid for Scientific Research(B)to Toshikazu Yoshikawa(Grant No.21390184)Challenging Exploratory Research to Yuji Naito(No.08101559)from the Japan Society for the Promotion of Science+1 种基金A City Area Program to Toshikazu Yoshikawa and Yuji Naito from Ministry of Education,Culture,Sports,Science and Technology,JapanAn Adaptable and Seamless Technology Transfer Program through targetdriven R&D to Yuji Naito from Japan Science and Technology Agency
文摘AIM:To investigate the efficacy of rebamipide in a rat model of colitis and restitution of intestinal epithelial cells in vitro.METHODS:Acute colitis was induced with trinitrobenzene sulfonic acid(TNBS)in male Wistar rats.Rats received intrarectal rebamipide treatment daily starting on day 7 and were sacrificed on day 14 after TNBS administration.The distal colon was removed to evaluate the various parameters of inflammation.Moreover,wound healing assays were used to determine the enhanced restitution of rat intestinal epithelial(RIE)cells treated with rebamipide.RESULTS:Intracolonic administration of rebamipide accelerated TNBSinduced ulcer healing.Increases in the wet weight of the colon after TNBS administration were significantly inhibited by rebamipide.The wound assay revealed that rebamipide enhanced the migration of RIE cells through phosphorylation of extracellular signalregulated kinase(ERK)and activation of Rho kinase.CONCLUSION:Rebamipide enema healed intestinal injury by enhancing restitution of RIE cells,via ERK activation.Rebamipide might be a novel therapeutic approach for inflammatory bowel disease.
