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复合性感染的复数菌药敏实验分析
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作者 桓新 马颖 +2 位作者 马瑞 朱敏 李蓉 《中国微生态学杂志》 CAS CSCD 2001年第5期293-294,共2页
关键词 复合性感染 药敏实验 复数菌
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TT virus and hepatitis G virus infections in Korean blood donors and patients with chronic liver disease 被引量:7
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作者 Mee Juhng Jeon Jong Hee Shin +2 位作者 Soon Pal Suh Young Chai Lim Dong Wook Ryang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第4期741-744,共4页
AIM:To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea,to investigate the association of TTV and HGV infections with blood transfusion,and to ass... AIM:To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea,to investigate the association of TTV and HGV infections with blood transfusion,and to assess the correlation between TTV and HGV viremia and hepatic damage. METHODS:A total of 391 serum samples were examined in this study.Samples were obtained from healthy blood donors(n=110),hepatitis B surface antigen(HBsAg)-positive donors(n=112),anti-hepatitis C virus(anti-HCV)-positive donors(n=69),patients with type B chronic liver disease (n=81),and patients with type C chronic liver disease(n=19). Trv DNA was detected using the hemi-nested PCR.HGV RNA was tested using RT-PCR.A history of blood transfusion and serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were also determined. RESULTS:TTV DNA was detected in 8.2%of healthy blood donors,16.1%of HBsAg-positive donors,20.3%of anti- HCV-positive donors,21.0%of patients with type B chronic liver disease,and 21.1%of patients with type C chronic liver disease.HGV RNA was detected in 1.8%of healthy blood donors,1.8%of HBsAg-positive donors,17.4%of anti-HCV-positive donors,13.6%of patients with type B chronic liver disease,and 10.5%of patients with type C chronic liver disease.The prevalence of TTV and HGV infections in HBV- or HCV-positive donors and patients was significantly higher than in healthy blood donors(P<0.05), except for the detection rate of HGV in HBsAg-positive donors which was the same as for healthy donors.There was a history of transfusion in 66.7%of TTV DNA-positive patients and 76.9%of HGV RNA-positive patients(P<0.05).No significant increase in serum ALT and AST was detected in the TTV or HGV-positive donors and patients. CONCLUSION:TTV and HGV infections are more frequently found in donors and patients infected with HBV or HCV than in healthy blood donors.However,there is no significant association between TTV or HGV infections and liver injury. 展开更多
关键词 Blood Donors Blood Transfusion Chronic Disease DNA Virus Infections DNA Viral Flaviviridae Infections GB virus C purification Hepatitis B Surface Antigens Hepatitis Viral Human Korea Liver Diseases Polymerase Chain Reaction Reference Values Reverse Transcriptase Polymerase Chain Reaction Torque teno virus
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Construction of a chimeric hepatitis C virus replicon based on a strain isolated from a chronic hepatitis C patient 被引量:1
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作者 Huang Cao Wandi Zhu +2 位作者 Qingxia Han Rongjuan Pei Xinwen Chen 《Virologica Sinica》 SCIE CAS CSCD 2014年第1期61-70,共10页
Subgenomic replicons of hepatitis C virus (HCV) have been widely used for studying HCV replication.Here,we report a new subgenomic replicon based on a strain isolated from a chronically infected patient.The coding s... Subgenomic replicons of hepatitis C virus (HCV) have been widely used for studying HCV replication.Here,we report a new subgenomic replicon based on a strain isolated from a chronically infected patient.The coding sequence of HCV was recovered from a Chinese chronic hepatitis C patient displaying high serum HCV copy numbers.A consensus sequence designated as CCH strain was constructed based on the sequences of five clones and this was classified by sequence alignment as belonging to genotype 2a.The subgenomic replicon of CCH was replication-deficient in cell culture,due to dysfunctions in NS3 and NS5B.Various JFH1/CCH chimeric replicons were constructed,and specific mutations were introduced.The introduction of mutations could partially restore the replication of chimeric replicons.A replication-competent chimeric construct was finally obtained by the introduction of NS3 from JFH1 into the backbone of the CCH strain. 展开更多
关键词 hepatitis C virus subgenomic replicon MUTATION
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