本研究采用了2个能值体系(代谢能、净能)×2种油脂来源(大豆油、复合油脂)的析因试验,探讨了在保育猪饲粮中添加复合油脂等量替代大豆油的可行性。试验选用了192头初始体重为(8.40±1.04)kg的健康杜×长×大三元断奶仔...本研究采用了2个能值体系(代谢能、净能)×2种油脂来源(大豆油、复合油脂)的析因试验,探讨了在保育猪饲粮中添加复合油脂等量替代大豆油的可行性。试验选用了192头初始体重为(8.40±1.04)kg的健康杜×长×大三元断奶仔猪,根据初始体重按照随机区组的原则分为4个处理,每个处理6个重复(栏),每个重复8头猪(公母各占1/2)。根据NRC(2012)推荐,分别采用净能和代谢能2套能值体系,配制7~11 kg和12~25 kg 2个阶段饲粮。对照组为玉米-豆粕型基础饲粮,试验组用2%的复合油脂等量替代2%的大豆油,饲喂35 d。饲养结束后,从饲喂净能体系饲粮的2个组中分别选择6头体重接近重复平均体重的猪屠宰,采集空肠组织做形态学观测,收集空肠黏膜、结肠内容物和新鲜粪便,分别用于检测紧密连接蛋白表达、抗氧化指标、挥发性脂肪酸含量以及微生物组成。结果表明:饲粮中添加2%的复合油脂替代等量的大豆油对保育猪的生长性能和腹泻率没有显著影响(P>0.05);复合油脂代替大豆油有提高血清免疫球蛋白G(IgG)(P=0.09)和免疫球蛋白M(IgM)含量(P=0.06)的趋势,但对血清中炎症因子含量没有显著影响(P>0.05),血清中脂质过氧化物丙二醛(MDA)含量显著降低(P<0.05),而且血清总抗氧化能力(T-AOC)(P=0.06)和谷胱甘肽过氧化物酶(GSH-Px)活性(P=0.06)也有升高的趋势。代谢能体系饲粮饲喂的猪的血清总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)和GSH-Px活性以及T-AOC显著高于净能体系饲粮饲喂的猪(P<0.05)。净能体系下,复合油脂等量替代大豆油显著升高了猪肠道黏膜中GSH-Px活性(P<0.05),有升高T-AOC(P=0.09)和CAT活性(P=0.08)的趋势;复合油脂组紧密连接蛋白-1(Claudin-1)和闭锁小带蛋白-2(ZO-2)的蛋白表达显著提高(P<0.05),闭合蛋白(Occludin)的蛋白表达也有升高的趋势(P=0.09);复合油脂等量代替大豆油显著提高了猪结肠内容物中乙酸的含量(P<0.05),而且粪便微生物门水平的Beta多样性显著变化(P<0.05),表现为采食复合油脂显著提高了厚壁菌门(Firmicutes)的相对丰度(P<0.05),显著降低了拟杆菌门(Bacteroidota)的相对丰度(P<0.05)。综上所述,保育猪饲粮中添加2%的复合油脂等量替代大豆油,不影响保育猪的生长性能和健康,而且有助于提高机体免疫力、抗氧化能力和维护肠道屏障功能。展开更多
Previous study has shown that 10-hydroxycamptothecin（HCPT） has well-established pharmacological effects in vitro.However,its in vivo bioavailability is very poor due to various problems,which severely restricts its ...Previous study has shown that 10-hydroxycamptothecin（HCPT） has well-established pharmacological effects in vitro.However,its in vivo bioavailability is very poor due to various problems,which severely restricts its clinical applications.In the present study,phospholipid complex（PC） technology was employed to improve the solubility and bioavailability of HCPT.XRD data confirmed the formation of HCPT-PC.However,our previously prepared HCPT-PC is too sticky,which may result in the slow dissolution rate and negative effects on its absorption.Therefore,we prepared HCPT-PC-solid dispersion（HCPT-PC-SD）and lipid-based formulations of HCPT-PC through simple preparation process.The results showed that the dissolution rate of HCPT-PC was effectively improved by solid dispersion technology,which reached 91.73%in 45 min.Pharmacokinetic study revealed that the AUC_（0-t） of HCPT-PC-SD and HCPT-PC lipid-based formulations was effectively further increased compared with HCPT-PC.Moreover,we found that the combination of SD technology and lipid-base formulations could be a promising drug-delivery system to improve the oral bioavailability of HCPT-PC.In addition,we showed that the bioavailability of HCPT-PC lipid-base formulations was even greater than that of HCPT-PC-SD.In particular,lipid-base formulations could be prepared just by a simple method,suggesting its feasibility of industrialization.展开更多
文摘本研究采用了2个能值体系(代谢能、净能)×2种油脂来源(大豆油、复合油脂)的析因试验,探讨了在保育猪饲粮中添加复合油脂等量替代大豆油的可行性。试验选用了192头初始体重为(8.40±1.04)kg的健康杜×长×大三元断奶仔猪,根据初始体重按照随机区组的原则分为4个处理,每个处理6个重复(栏),每个重复8头猪(公母各占1/2)。根据NRC(2012)推荐,分别采用净能和代谢能2套能值体系,配制7~11 kg和12~25 kg 2个阶段饲粮。对照组为玉米-豆粕型基础饲粮,试验组用2%的复合油脂等量替代2%的大豆油,饲喂35 d。饲养结束后,从饲喂净能体系饲粮的2个组中分别选择6头体重接近重复平均体重的猪屠宰,采集空肠组织做形态学观测,收集空肠黏膜、结肠内容物和新鲜粪便,分别用于检测紧密连接蛋白表达、抗氧化指标、挥发性脂肪酸含量以及微生物组成。结果表明:饲粮中添加2%的复合油脂替代等量的大豆油对保育猪的生长性能和腹泻率没有显著影响(P>0.05);复合油脂代替大豆油有提高血清免疫球蛋白G(IgG)(P=0.09)和免疫球蛋白M(IgM)含量(P=0.06)的趋势,但对血清中炎症因子含量没有显著影响(P>0.05),血清中脂质过氧化物丙二醛(MDA)含量显著降低(P<0.05),而且血清总抗氧化能力(T-AOC)(P=0.06)和谷胱甘肽过氧化物酶(GSH-Px)活性(P=0.06)也有升高的趋势。代谢能体系饲粮饲喂的猪的血清总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)和GSH-Px活性以及T-AOC显著高于净能体系饲粮饲喂的猪(P<0.05)。净能体系下,复合油脂等量替代大豆油显著升高了猪肠道黏膜中GSH-Px活性(P<0.05),有升高T-AOC(P=0.09)和CAT活性(P=0.08)的趋势;复合油脂组紧密连接蛋白-1(Claudin-1)和闭锁小带蛋白-2(ZO-2)的蛋白表达显著提高(P<0.05),闭合蛋白(Occludin)的蛋白表达也有升高的趋势(P=0.09);复合油脂等量代替大豆油显著提高了猪结肠内容物中乙酸的含量(P<0.05),而且粪便微生物门水平的Beta多样性显著变化(P<0.05),表现为采食复合油脂显著提高了厚壁菌门(Firmicutes)的相对丰度(P<0.05),显著降低了拟杆菌门(Bacteroidota)的相对丰度(P<0.05)。综上所述,保育猪饲粮中添加2%的复合油脂等量替代大豆油,不影响保育猪的生长性能和健康,而且有助于提高机体免疫力、抗氧化能力和维护肠道屏障功能。
基金Science and Technology Department of Henan province Fund Project(Grant No.144300510019)
文摘Previous study has shown that 10-hydroxycamptothecin（HCPT） has well-established pharmacological effects in vitro.However,its in vivo bioavailability is very poor due to various problems,which severely restricts its clinical applications.In the present study,phospholipid complex（PC） technology was employed to improve the solubility and bioavailability of HCPT.XRD data confirmed the formation of HCPT-PC.However,our previously prepared HCPT-PC is too sticky,which may result in the slow dissolution rate and negative effects on its absorption.Therefore,we prepared HCPT-PC-solid dispersion（HCPT-PC-SD）and lipid-based formulations of HCPT-PC through simple preparation process.The results showed that the dissolution rate of HCPT-PC was effectively improved by solid dispersion technology,which reached 91.73%in 45 min.Pharmacokinetic study revealed that the AUC_（0-t） of HCPT-PC-SD and HCPT-PC lipid-based formulations was effectively further increased compared with HCPT-PC.Moreover,we found that the combination of SD technology and lipid-base formulations could be a promising drug-delivery system to improve the oral bioavailability of HCPT-PC.In addition,we showed that the bioavailability of HCPT-PC lipid-base formulations was even greater than that of HCPT-PC-SD.In particular,lipid-base formulations could be prepared just by a simple method,suggesting its feasibility of industrialization.
