基因工程猪生长激素复性技术的研究

对基因工程猪生长激素 (r pGH)体外复性条件进行研究 ,提取包涵体用 6mol L盐酸胍溶解 ,空气氧化 80h后转入复性缓冲液中开始重组蛋白复性 ,结果表明 ,采用透析复性法———复性缓冲液Ⅲ ( 0 2 5 %Na2 CO3 ,0 2 %乳糖 ,0 2 %甘露醇 ) ,pH8 5 ,透析 5～ 6次 ,可较好恢复r pGH生物活性。β 巯基乙醇、谷光苷肽等添加剂对r pGH的体外复性影响不显著。复性后蛋白浓缩液进行去垂体大鼠试验 ,大鼠增重明显 ,表明得到了正确折叠的较高生物活性的r pGH。

蛋白质复性技术

重组蛋白质的过表达常导致其在胞内发生错误折叠和聚集，形成被称为包含体的聚集体。因此，蛋白质复性是许多基因重组蛋白质药物生产过程的重要步骤。本文简要介绍包含体提取、纯化和溶解工艺，重点阐述蛋白质复性技术，包括稀释复性、稀释添加剂、人工分子伴侣、柱色谱复性和反胶团溶解复性等。最后展望蛋白质复性技术的发展和应用，特别是荷电介质对同电荷蛋白质复性的促进作用。

湖南柑桔冻害频率与冻后恢复性技术措施

分析了湖南省516 a来的柑桔冻害频率,结果表明,大冻害发生频率建国前为114.25 a一次,建国后为9.83 a一次,建国后湖南省柑桔大冻害频率显著提高是柑桔生长的生态环境变化所致。柑桔冻害程度与低温及持续时间、小气候及大环境生态条件、柑桔培管水平及冻前结果量、降温前气候及土壤湿度、柑桔品种及熟期等因素密切相关。针对2008年1月湖南省柑桔遭受特大冻害的情况,提出农技推广部门要指导桔农辨别柑桔冻害程度,传授恢复性技术措施,对于3级以上冻害的桔树,结合品种优化,考虑高接换种。并推荐了柑桔冻后的恢复性技术措施。

包涵体蛋白的复性技术

细菌表达系统产生的重组蛋白常为不溶的包涵体蛋白，影响产率。随着对蛋白质折叠中凝聚机制的认识，在包涵体复性中对一些条件加以控制，如蛋白质浓度、缓冲液的组成等，特别是低分子量添加剂的使用，可使蛋白产率提高。

重组蛋白的体外复性技术研究进展

概要介绍了重组蛋白体外复性的意义，该技术的难点及现有复性方法的局限性。并重点阐述了色谱复性和分子伴侣体外复性技术的原理、特点及在基因工程药物生产过程中的应用，结果表明新型重组蛋白复性技术可将复性收率提高80％－100％，有望解决基因工程产业化的关键技术。

发现新基因的高效方法——cDNA文库的复性式均一化技术

由复性式均一化技术制作的均一化cDNA文库 (equalizedcDNAlibrary ,normalizedcDNAlibrary)是近年来发展起来的一种获得EST、发现新基因的高效平台。本文就该技术的原理、方法比较、存在问题和展望进行了阐述。

用复性电泳技术研究溶酶体蛋白水解酶的性质和活性

介绍了复性单向电泳（Ｇ－ＰＡＧＥ）和复性双向电泳（ＩＥＦ－Ｇ－ＰＡＧＥ）的概念、原理、方法、应用范围、优缺点和使用该方法应注意的事项。并用该方法定性和定量分析了Ｃ６大白鼠神经胶质瘤细胞酸性溶酶体蛋白水解酶的性质和活性。

延边黄牛背最长肌差异表达基因的筛选、克隆及序列分析

应用引物复性控制技术筛选肌内脂肪含量差异极显著的延边黄牛背最长肌组织差异表达基因,寻找与肌内脂肪沉积的相关候选基因。文章选取30头28月龄延边黄牛阉牛的背最长肌组织测定肌内脂肪含量,选取肌内脂肪含量差异极显著的最高和最低各3头组成RNA池,采用引物复性控制技术,分析了两组个体背最长肌组织差异表达基因。利用20对随机引物差异显示扩增下,共获得12条ESTs(片段大小为200～890 bp),其中8个为已知的ESTs分别与细胞骨架形成、细胞因子信号转导、蛋白质合成、能量代谢和其他功能的差异基因,4个未知的ESTs。结果表明,应用引物复性控制技术筛选得到了12个可能参与了肌内脂肪沉积调控的ESTs,为进一步筛选肌内脂肪沉积相关的基因奠定了基础。

水稻新品种玉稻518灌浆期POD动态分析

采用复性电泳技术对离子束诱变的水稻新品种玉稻518及其亲本在灌浆期的POD酶谱动态变化进行了分析,结果表明,稳定遗传的诱变后代与亲本灌浆期叶片的POD酶谱存在显著差异:1)诱变后代检出185、54kD和42kD3条新酶带;2)诱变后代中245、53kD和38kD酶带较亲本表达时期有差异;3)诱变后代灌浆中期、后期POD总酶活较亲本显著增强。

Multi-Bit Sliding Stack Decoding Algorithm for OVXDM

Overlapped x domain multiplexing(OVXDM) is a promising encoding technique to obtain high spectral efficiency by utilizing inter-symbol interference(ISI) intelligently. However, the computational complexity of maximum likelihood sequence detection(MLSD) increases exponentially with the growth of spectral efficiency in OVXDM, which is unbearable for practical implementations. In this paper, based on a novel path metric associating adjacent symbols, we propose a multi-bit sliding stack decoding(Multi-Bit SSD) algorithm to achieve multiple-bit decoding simultaneously in OVXDM. Theoretical analysis is provided for the algorithm, which indicates the relationship between the performance and parameters including multiplexing waveform, overlapping fold and sliding window size. Simulation results show that the proposed algorithm can achieve better decoding performance and higher spectral efficiency than conventional fast decoding algorithms.

Electro-conductive Nanocrystalline Cellulose Film Filled with TiO_2-ReducedGraphene Oxide Nanocomposite

Imparting electro-conductive properties to nanocellulose-based products may render them suitable for applications in electronics, optoelectronics, and energy storage devices. In the present work, an electro-conductive nanocrystalline cellulose （NCC） film filled with TiO2-reduced-graphene oxide （TiO2-RGO） was developed. Initially, graphene oxide （GO） was prepared using the modified Hummers method and thereafter photocatalytically reduced using TiO2 as a catalyst. Subsequently, an electro-conductive NCC film was prepared via vacuum filtration with the as-prepared TiO2-RGO nanocomposite as a functional filler. The TiO2-RGO nanocomposite and the NCC/TiO2-RGO film were systematically characterized. The results showed that the obtained TiO2-RGO nanocomposite exhibited reduced oxygen-containing group content and enhanced electro-conductivity as compared with those of GO. Moreover, the NCC flm flled with TiO2-RGO nanocomposite displayed an electro-conductivity of up to 9.3 S/m and improved mechanical properties compared with that of the control. This work could provide a route for producing electro-conductive NCC flms, which may hold signifcant potential as transparent ？exible substrates for future electronic device applications.

Low-Complexity Detection and Decoding Scheme for LDPC-Coded MLC NAND Flash Memory

With the development of manufacture technology, the multi-level cell(MLC)technique dramatically increases the storage density of NAND flash memory. As the result,cell-to-cell interference(CCI) becomes more serious and hence causes an increase in the raw bit error rate of data stored in the cells.Recently, low-density parity-check(LDPC)codes have appeared to be a promising solution to combat the interference of MLC NAND flash memory. However, the decoding complexity of the sum-product algorithm(SPA) is extremely high. In this paper, to improve the accuracy of the log likelihood ratio(LLR) information of each bit in each NAND flash memory cell, we adopt a non-uniform detection(N-UD) which uses the average maximum mutual information to determine the value of the soft-decision reference voltages.Furthermore, with an aim to reduce the decoding complexity and improve the decoding performance, we propose a modified soft reliabilitybased iterative majority-logic decoding(MSRBI-MLGD) algorithm, which uses a non-uniform quantizer based on power function to decode LDPC codes. Simulation results show that our design can offer a desirable trade-off between the performance and complexity for high-column-weight LDPC-coded MLC NAND flash memory.

Thermal Treatment of Hydrocarbon-Impacted Soils＂ A Review of Technology Innovation for Sustainable Remediation

Thermal treatment technologies hold an important niche in the remediation of hydrocarbon- contaminated soils and sediments due to their ability to quickly and reliably meet cleanup standards. However, sustained high temperature can be energy intensive and can damage soil properties. Despite the broad applicability and prevalence of thermal remediation, little work has been done to improve the environmental compatibility and sustainahility of these technologies. We review several common thermal treatment technologies for hydrocarbon-contaminated soils, assess their potential environmental impacts, and propose frameworks for sustainable and low-impact deployment based on a holistic consideration of energy and water requirements, ecosystem ecology, and soil science. There is no universally appropriate thermal treatment technology. Rather, the appropriate choice depends on the contamination scenario （including the type of hydrocarbons present） and on site-specific considerations such as soil properties, water availability, and the heat sensitivity of contaminated soils. Overall, the convergence of treatment process engineering with soil science, ecosystem ecology, and plant biology research is essential to fill critical knowledge gaps and improve both the removal efficiency and sustainability of thermal technologies.

RELAY ASSISTED TRANSMISSSION WITH BIT-INTERLEAVED CODED MODULATION

We investigate an adaptive cooperative protocol in a Two-Hop-Relay (THR) wireless system that combines the following: (1) adaptive relaying based on repetition coding; (2) single or two transmit antennas and one receive antenna configurations for all nodes, each using high order constellation; (3) Bit-Interleaved Coded Modulation (BICM). We focus on a simple decoded relaying (i.e. no error correcting at a relay node) and simple signal quality thresholds for relaying. Then the impact of the two simple thresholds on the system performance is studied. Our results suggest that compared with the traditional scheme for direct transmission, the proposed scheme can increase average throughput in high spectral efficiency region with low implementa-tion-cost at the relay.

Rehabilitation Soils with Date Palm Mulching Treatments

Kuwait is located in hyper arid desert environment. This geographical location and irrational human activities accelerate the expansion of land degradation problem. In order to rehabilitate the degraded areas it is necessary to use soil and water resources in sustainable manner. Owing to these reasons it is essential to use appropriate methods based on the scientific diagnostics of the problem. It is compulsory to identify, specify and test different efficient, cost-effective and environment friendly sound sustainable sand control measures such as, semi-circular bunds, square micro catchment and checker board palm leaves to rehabilitate degraded lands in Liyah refilled quarries. To evaluate the impact of rehabilitation method through mulching with date palm leaves soil samples were collected （control and rehabilitation sites with square micro catchment） prior to the implementation plan and post completion of the project （three years）. In this study will be highlighted on detail the results of using square micro catchment and with short brief descriptions on other water harvesting techniques. The results showed an improvement of physical soil properties after the application of these techniques. Soil fertility increased through increasing the quantity of fine and very fine sand. The soil moisture at the surface soil increased from 0.085% to 1.62% after the treatment. The untreated soil has high bulk density about 2 g/cm3 and low porosity about 27%. However, after the soil was ploughed and mulched the bulk density decreased to 0.03 g/cm3. And porosity improved 98%. This study conserved the irrigation water through reducing the proportion of soil evaporation, in addition to the creation of optimum condition for plant growth. The aim of this study was to analyze the effect of using organic mulch date palm leaves with water harvesting techniques on the physical and biological properties of degraded soil in Liyah area.

Sustainable development of resource-based industrial clusters in Yunnan,China

The resources-oriented industry cluster is geographically close to natural resources development and processing industry.The knowledge-intensive,technology-intensive,capital-intensive,and labor-intensive is a relatively aggregate associated with natural resources and will structure commonality and complementary coupling so that economic and social development play a leading role in the complex adaptive systems closely related to ecological environment.A resources-oriented industry cluster in Yunnan has general common characteristics of industrial clusters and will form the characteristics of the enterprise spatial aggregation of innovative network of the industrial chain and value chain.According to the market structure of resources and resources market behavior in Yunnan,the particularity of resources commodity production has conduced resources market structure and general merchandise market structure many differences.Optimizing the cluster structure and extending the industrial chain have improved the productivity of the cluster and extension the lifecycle of the cluster to achieve sustainable development.

Variation of DRB1 Gene in Tibetan Sheep

It reveals that the MHC （major histocompatibility complex） gene product always involved in the control of immune response and disease resistance. Nowadays many studies have indicated the OLA （ovine lymphocyte antigen） DRB1 gene is associated with some sheep diseases. Tibetan sheep is one of the three major shag sheep breeds in China, and also have the largest number of China＇s sheep breeds. But till now no report has been seen on studying DRB1 gene in Tibetan sheep of China. To understand the evolution and provide the basis for sheep disease resistance, polymorphism in the exon2 ofDRB1 gene in Tibetan sheep was analyzed. The PCR-SSCP, cloning and sequencing were used to analyse DRB1 gene variation in 600 Tibetan sheep of China. And the genetic relationship and evolutionary significance of the alleles had also been analyzed. Total of 31 alleles were identified, in which 15 alleles had not been reported before. And there were 70 SNPs （single nucleotide polymorphisms） sites in 31 sheep DRB1 gene haplotypes, the proportion was 29.5% to the whole exort2 sequence. All of this indicated that DRB1 exon2 is highly polymorphic in Tibetan sheep. The variation identified here might have an impact on both the function and level of expression of the OLA-DRB1.

Influence of modulated structure on magnetic properties of NdFeB/Co multilayer thin films

The Nd Fe B/Co multilayer films were prepared by magnetron sputtering. After that, the samples were annealed at 600 ℃ for 20 min. The surface morphology, phase structures and magnetic properties of Mo（50 nm）/[Nd Fe B（100 nm）/Co（y）]×10/Mo（50 nm） thin films were researched by AFM, XRD and VSM, respectively. The results show that the films show stronger perpendicular magnetic anisotropy. When the thickness of Co layers is 10 nm, the coercivity Hc⊥ is the maximum, 295 k A/m. However, for y=10-20, the reduced remanence M/Ms of films has increased. When the thickness of Co layers is 20-30 nm, the Nd Fe B/Co multilayer films obtained more superior magnetic properties with M/Ms =0.95.

The Repairing of Concrete Walls in Structure for Water Treatment in Thermo Power Plant

As a result of longer term exploitation, exposure to severe weather conditions or influence of chemical conditions, concrete walls of concrete structures get damaged internally as well as externally. This study includes a preliminary analysis of chemically treated water in existing concrete structures, and another after the application of the method and using materials for the structures in future. One of the priorities was to examine the existing concrete structures by using nondestructive and destructive methods. After that, based on the results of the analysis, adequate new materials are proposed for the repairs, most commonly new technology polymer carbonated materials, in order to achieve durability of structure elements in using technological processes. Behavior of the repairing structures was tested using the in situ methods, and especially pull-of test, to verify the adhesion force between the old concrete structures and new applied layer. After the repairing, the concrete structures will be monitored to record the behavior under the chemical treated water.