Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321&#...Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties.展开更多
The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, h...The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, have been elaborated. The cultivation of explants was carried out on the Gamborg （B5） feeding area. The hormonal （BAP; Zn; NAA） composition of the feeding area and their concentrations have been selected; proliferation of buds in the basal part of the sprout has been achieved from the formed morphogenic tissue. The microclones by activating axillary meristem have been received.展开更多
In order to compare the differences in the accumulation of plumbum(Pb),cadmium(Cd),chromium(Cr),cuprum(Cu),nickel(Ni)and zinc(Zn)from the multi-contaminated,alkalescent soil in the plants of four-season big-leaf coria...In order to compare the differences in the accumulation of plumbum(Pb),cadmium(Cd),chromium(Cr),cuprum(Cu),nickel(Ni)and zinc(Zn)from the multi-contaminated,alkalescent soil in the plants of four-season big-leaf coriander,butter lettuce,four-season fast-growing pakchoi and four-season parsley to choose low accumulation vegetables,a pot experiment was conducted by using heavy metal salts to simulate high-,moderate-and low-level heavy metals multi-contaminated soils.The results showed that the multi-contaminated soil by high levels of Pb,Cd,Cr,Cu and Zn produced inhibition on biomass of four-season fast-growing pakchoi,failing to collect samples,but had no obvious inhibition on the growth of the three other vegetables.Among the four kinds of vegetables examined,the accumulation ability of four-season parsley to the five heavy metals of Pb,Cd,Cr,Cu and Zn was the strongest,while the accumulation ability of four-season fast-growing pakchoi to Ni was the strongest.In the high-level contaminated soil,the underground part of these four vegetables grown was more likely to accumulate heavy metals than their aboveground part.Both of vegetable varieties and heavy metal types can affect the accumulation of heavy metals in vegetables.Our research also found that four-season big-leaf coriander was high yielding and low accumulation of heavy metals.展开更多
文摘Hypocotyl segments from aseptic seedlings of two important cultivars of upland cotton ( Gossypium hirsutum L.) in Northwest China, 'Xinluzao_1', 'Jinmian_7', 'Jinmian_12' and 'Jihe_321' were transformed respectively by two efficient plant expression plasmids pBinMoBc and pBinoBc via Agrobacterium tumefaciens . In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter. After co_cultivation with Agrobacterium tumefimpfaciens LBA4404 (containing pBinMoBc or pBinoBc), kanamycin_resistant selection, somatic embryos were induced and regenerated plants were obtained. Then the regenerated plantlets were grafted to untransformed stocks in greenhouse to produce descendants. The integration of cry 1Ac3 gene and its expression in T 2 generation of transgenic cotton plants were confirmed by Southern hybridization and Western blotting. The analyses of insect bioassay indicated that the transgenic plants of both constructions have significant resistance to the larvae of cotton bollworm ( Heliothis armigera ) and that cry 1Ac3 gene driven by chimeric OM promoter could endue T 2 generation cotton with high pest_resistant ability, implicating that it has a profound application in genetic engineering to breed new pest_resistant cotton varieties.
文摘The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, have been elaborated. The cultivation of explants was carried out on the Gamborg （B5） feeding area. The hormonal （BAP; Zn; NAA） composition of the feeding area and their concentrations have been selected; proliferation of buds in the basal part of the sprout has been achieved from the formed morphogenic tissue. The microclones by activating axillary meristem have been received.
文摘In order to compare the differences in the accumulation of plumbum(Pb),cadmium(Cd),chromium(Cr),cuprum(Cu),nickel(Ni)and zinc(Zn)from the multi-contaminated,alkalescent soil in the plants of four-season big-leaf coriander,butter lettuce,four-season fast-growing pakchoi and four-season parsley to choose low accumulation vegetables,a pot experiment was conducted by using heavy metal salts to simulate high-,moderate-and low-level heavy metals multi-contaminated soils.The results showed that the multi-contaminated soil by high levels of Pb,Cd,Cr,Cu and Zn produced inhibition on biomass of four-season fast-growing pakchoi,failing to collect samples,but had no obvious inhibition on the growth of the three other vegetables.Among the four kinds of vegetables examined,the accumulation ability of four-season parsley to the five heavy metals of Pb,Cd,Cr,Cu and Zn was the strongest,while the accumulation ability of four-season fast-growing pakchoi to Ni was the strongest.In the high-level contaminated soil,the underground part of these four vegetables grown was more likely to accumulate heavy metals than their aboveground part.Both of vegetable varieties and heavy metal types can affect the accumulation of heavy metals in vegetables.Our research also found that four-season big-leaf coriander was high yielding and low accumulation of heavy metals.
