目的观察夏朴消瘿方(XPXYF)含药血清对碘化钠(NaI)诱导的人甲状腺上皮细胞(Nthy ori 3-1)焦亡的影响。方法将Nthy ori 3-1细胞分为3组,空白组不予任何干预措施,模型组用NaI诱导细胞焦亡模型,实验组在造模的基础上加入含药血清培养。CCK-...目的观察夏朴消瘿方(XPXYF)含药血清对碘化钠(NaI)诱导的人甲状腺上皮细胞(Nthy ori 3-1)焦亡的影响。方法将Nthy ori 3-1细胞分为3组,空白组不予任何干预措施,模型组用NaI诱导细胞焦亡模型,实验组在造模的基础上加入含药血清培养。CCK-8法筛选含药血清最佳浓度,流式细胞术检测Nthy ori 3-1细胞焦亡,LDH法检测细胞毒性,细胞电镜观察Nthy ori 3-1细胞形态变化,流式细胞术检测氧化应激指标[活性氧(ROS)],Western blotting法检测细胞焦亡相关蛋白[焦亡蛋白D(GSDMD)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、Caspase-1、核因子κB(NF-κB)]表达。结果与空白对照组比较,模型组细胞活力低(P<0.01);与模型组比较,实验组细胞活力高(P<0.01),实验组10%含药血清干预48 h,Nthy ori 3-1细胞活力最高。因此将10%含药血清用于后续研究。与空白对照组比较,模型组FLICA(+)-PI(+)双阳性比例高,焦亡率高(P<0.01)。与模型组比较,实验组FLICA(+)-PI(+)双阳性比例低,焦亡率低(P<0.05)。与空白对照组比较,模型组Nthy ori 3-1细胞LDH释放量多(P<0.01);与模型组比较,实验组细胞毒性少(P<0.01)。实验组甲状腺滤泡细胞形态较规则,细胞核变小,胞质内空泡形成,空泡较模型组少,线粒体呈椭圆形,线粒体结构比模型组较完整。与空白对照组比较,模型组ROS水平高(P<0.01);与模型组比较,实验组ROS水平低(P<0.01)。与空白对照组比较,模型组GSDMD、NF-κB p56蛋白表达高(P<0.05);与模型组比较,实验组GSDMD、Caspase-1、NF-κB p56、NLRP3蛋白表达低(P<0.05)。结论XPXYF含药血清可改善高碘诱导的Nthy ori 3-1细胞焦亡,可能与抑制氧化应激及激活GSDMD/NF-κB信号通路有关。展开更多
Objective： To observe the therapeutic effect and mechanism of modified Banxia Houpu decoction on globus hystericus. Methods： The 95 patients with globus hystericus were randomly divided into a treatment group of 46 ...Objective： To observe the therapeutic effect and mechanism of modified Banxia Houpu decoction on globus hystericus. Methods： The 95 patients with globus hystericus were randomly divided into a treatment group of 46 cases treated with modified Banxia Houpu decoction and a control group of 49 cases treated with Manyanshuning （Granula for Clearing the Throat）. In addition, a normal group of 24 healthy people was set up. SCL-90 scale was adopted to observe the therapeutic effect, evaluate the psychological state of patients and build a database on combination of four diagnoses. Results： The effect of the modified Banxia Houpo decoction was better than that of the control group in relieving depression, anxiety and improving the psychological state （P〈0.05 or P〈0.01）. Conclusion： Modified Banxia Houpu decoction has definite therapeutic effect on globus hystericus. Its mechanism may be related to its function in relieving depression and anxiety and regulating the osvchological state.展开更多
文摘目的观察夏朴消瘿方(XPXYF)含药血清对碘化钠(NaI)诱导的人甲状腺上皮细胞(Nthy ori 3-1)焦亡的影响。方法将Nthy ori 3-1细胞分为3组,空白组不予任何干预措施,模型组用NaI诱导细胞焦亡模型,实验组在造模的基础上加入含药血清培养。CCK-8法筛选含药血清最佳浓度,流式细胞术检测Nthy ori 3-1细胞焦亡,LDH法检测细胞毒性,细胞电镜观察Nthy ori 3-1细胞形态变化,流式细胞术检测氧化应激指标[活性氧(ROS)],Western blotting法检测细胞焦亡相关蛋白[焦亡蛋白D(GSDMD)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、Caspase-1、核因子κB(NF-κB)]表达。结果与空白对照组比较,模型组细胞活力低(P<0.01);与模型组比较,实验组细胞活力高(P<0.01),实验组10%含药血清干预48 h,Nthy ori 3-1细胞活力最高。因此将10%含药血清用于后续研究。与空白对照组比较,模型组FLICA(+)-PI(+)双阳性比例高,焦亡率高(P<0.01)。与模型组比较,实验组FLICA(+)-PI(+)双阳性比例低,焦亡率低(P<0.05)。与空白对照组比较,模型组Nthy ori 3-1细胞LDH释放量多(P<0.01);与模型组比较,实验组细胞毒性少(P<0.01)。实验组甲状腺滤泡细胞形态较规则,细胞核变小,胞质内空泡形成,空泡较模型组少,线粒体呈椭圆形,线粒体结构比模型组较完整。与空白对照组比较,模型组ROS水平高(P<0.01);与模型组比较,实验组ROS水平低(P<0.01)。与空白对照组比较,模型组GSDMD、NF-κB p56蛋白表达高(P<0.05);与模型组比较,实验组GSDMD、Caspase-1、NF-κB p56、NLRP3蛋白表达低(P<0.05)。结论XPXYF含药血清可改善高碘诱导的Nthy ori 3-1细胞焦亡,可能与抑制氧化应激及激活GSDMD/NF-κB信号通路有关。
基金supported by a grant from High-tech Project of Science & Technology Department of Jiangsu Province (No 30772839, No30873302)
文摘Objective： To observe the therapeutic effect and mechanism of modified Banxia Houpu decoction on globus hystericus. Methods： The 95 patients with globus hystericus were randomly divided into a treatment group of 46 cases treated with modified Banxia Houpu decoction and a control group of 49 cases treated with Manyanshuning （Granula for Clearing the Throat）. In addition, a normal group of 24 healthy people was set up. SCL-90 scale was adopted to observe the therapeutic effect, evaluate the psychological state of patients and build a database on combination of four diagnoses. Results： The effect of the modified Banxia Houpo decoction was better than that of the control group in relieving depression, anxiety and improving the psychological state （P〈0.05 or P〈0.01）. Conclusion： Modified Banxia Houpu decoction has definite therapeutic effect on globus hystericus. Its mechanism may be related to its function in relieving depression and anxiety and regulating the osvchological state.
