Objective The aim of the study was to explore the difference between immune cell subsets during the incubation of cytokine-induced kill cells （CIKs） from patients with and without hepatitis B virus （HBV）. Methods ...Objective The aim of the study was to explore the difference between immune cell subsets during the incubation of cytokine-induced kill cells （CIKs） from patients with and without hepatitis B virus （HBV）. Methods Peripheral blood samples were extracted from 50 tumor patients, and were divided into two groups according to the presence or absence of HBV. The proliferation rate and activity of CIK cells were examined based on counts on days 1, 5, 7, 9, 11, 13, and 15 of culture. Additionally, the CD3＋, CD4＋, CD8＋, CD3＋CD8＋, C＋）3＋CD4＋, and CD3＋CD56＋ T cell populations were analyzed by flow cytometry on days 5, 7, 10, 13, and 15 of culture. Results Proliferation over a 15-day period was higher in the HBV-positive group than in the negative group （280-fold vs. 180-fold increase, respectively）, but there was no significant difference between the two groups at each time point. The frequencies of CD3＋, CD8＋ T, CD3＋CD8＋, and CD3＋CD56＋T cells increased over time, while those of CD4＋ and CD3＋CD4＋ T cells decreased over time, and these changes were greater in the positive group than in the negative group. The differences in CD8＋ T cells and CD3＋CD4＋ T cells between the two groups were significant （P 〈 0.05）. Conclusion The proliferative capacity of CIK cells was higher for patients in the HBV-positive group than those in the HBV-negative group, and immune cell subsets were more favorable in the HBV-positive group than the neaative arouD.展开更多
The MTEC1 cell line, established in our laboratory, is a normal epithelial cell line derived from thymus medulla of Balb/c mice and these cells constitutively produce multiple cytokines. The selection of thymic microe...The MTEC1 cell line, established in our laboratory, is a normal epithelial cell line derived from thymus medulla of Balb/c mice and these cells constitutively produce multiple cytokines. The selection of thymic microenvironment on developing T cells was investigated in an in vitro system. Un-separated fresh thymocytes from Balb/c mice were cocul-tured with MTECl cells or/and MTEC1-SN,then,the viability, proliferation and phenotypes of cultured thymocytes were assessed. Without any exogenous stimulus, both MTECl cells and MTECl -SN were able to maintain the viability of thymocytes, while only the MTECl cells, not the MTECl -SN, could directly activate thymocytes to exhibit moderate proliferation, indicating that the proliferative signal is delivered through cell surface interactions of MTECl cells and thymocytes. Phenotype analysis on FACS of viable thymocytes after coculture revealed that MTECl cells preferentially activate the subsets of CD4+ CDS', CD4+ CD8+ and CD4- CD8- thymocytes; whereas MTEC1- SN preferentially maintained the viability of CD4+ CD8- and CD4-CD8+ thymocyte subsets.For the Con A-activated thymocytes, both MTEC1 cells and MTEC1-SN provided accessory signal(s) to significantly increase the number of viable cells and to markedly enhance the proliferation of thymocytes with virtually equal potency, phenotyped as CD4+CD8-, CD4-CD8+, and CD4- CD8- subsets. In summary, MTEC1 cells displayedselective support to the different thymocyte subsets , and the selectivity is dependent on the status of thymocytes.展开更多
Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combinati...Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combination on symbiotic. In this in vitro study the authors assess the effect of a symbiotic combination between cellobiose and two probiotic strains （L. rhamnosus, S. thermophilus） toward two pathogenic bacteria （S. aureus and E. colO. In addition, we evaluate the influence of this fiber either on the localization of the lactic inhibiting substances and their extracelluler polymer substances （EPS） production. The results showed that the prebiotic candidate noticeably elongated the adaptation phase of the target pathogens to up than 12 hours, while stimulate the probiotic growth till 2 Log CFU/mL in favor of L. rhamnosus and to approximately 3 Log CFU/mL for S. thermophilus compared with control substrate （glucose）. The inhibiting effect was more important toward S. aureus （2.54 and 1.09 Log CFU/mL against 4.99 and 7.75 in co-culture with L. rhamnosus and S. thermophilus respectively at the 4th hour of incubation） and lesser for E. coli compared with the control substrate. And the results suggested that S thermophilus inhibited both target strains mainly by bacteriocins, whilst L. rharnnosus rather inhibited S. aureus growth by bacteriocins and E. coli ones by acids production. We also observed a significant stimulation of the exopolysaccahrids production by this fiber, which may allow a better adhesion of the probiotic candidates in the intestinal tract.展开更多
文摘Objective The aim of the study was to explore the difference between immune cell subsets during the incubation of cytokine-induced kill cells （CIKs） from patients with and without hepatitis B virus （HBV）. Methods Peripheral blood samples were extracted from 50 tumor patients, and were divided into two groups according to the presence or absence of HBV. The proliferation rate and activity of CIK cells were examined based on counts on days 1, 5, 7, 9, 11, 13, and 15 of culture. Additionally, the CD3＋, CD4＋, CD8＋, CD3＋CD8＋, C＋）3＋CD4＋, and CD3＋CD56＋ T cell populations were analyzed by flow cytometry on days 5, 7, 10, 13, and 15 of culture. Results Proliferation over a 15-day period was higher in the HBV-positive group than in the negative group （280-fold vs. 180-fold increase, respectively）, but there was no significant difference between the two groups at each time point. The frequencies of CD3＋, CD8＋ T, CD3＋CD8＋, and CD3＋CD56＋T cells increased over time, while those of CD4＋ and CD3＋CD4＋ T cells decreased over time, and these changes were greater in the positive group than in the negative group. The differences in CD8＋ T cells and CD3＋CD4＋ T cells between the two groups were significant （P 〈 0.05）. Conclusion The proliferative capacity of CIK cells was higher for patients in the HBV-positive group than those in the HBV-negative group, and immune cell subsets were more favorable in the HBV-positive group than the neaative arouD.
基金grants from the China medical board, USA,and from the national foundation of natural scirnces,China
文摘The MTEC1 cell line, established in our laboratory, is a normal epithelial cell line derived from thymus medulla of Balb/c mice and these cells constitutively produce multiple cytokines. The selection of thymic microenvironment on developing T cells was investigated in an in vitro system. Un-separated fresh thymocytes from Balb/c mice were cocul-tured with MTECl cells or/and MTEC1-SN,then,the viability, proliferation and phenotypes of cultured thymocytes were assessed. Without any exogenous stimulus, both MTECl cells and MTECl -SN were able to maintain the viability of thymocytes, while only the MTECl cells, not the MTECl -SN, could directly activate thymocytes to exhibit moderate proliferation, indicating that the proliferative signal is delivered through cell surface interactions of MTECl cells and thymocytes. Phenotype analysis on FACS of viable thymocytes after coculture revealed that MTECl cells preferentially activate the subsets of CD4+ CDS', CD4+ CD8+ and CD4- CD8- thymocytes; whereas MTEC1- SN preferentially maintained the viability of CD4+ CD8- and CD4-CD8+ thymocyte subsets.For the Con A-activated thymocytes, both MTEC1 cells and MTEC1-SN provided accessory signal(s) to significantly increase the number of viable cells and to markedly enhance the proliferation of thymocytes with virtually equal potency, phenotyped as CD4+CD8-, CD4-CD8+, and CD4- CD8- subsets. In summary, MTEC1 cells displayedselective support to the different thymocyte subsets , and the selectivity is dependent on the status of thymocytes.
文摘Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combination on symbiotic. In this in vitro study the authors assess the effect of a symbiotic combination between cellobiose and two probiotic strains （L. rhamnosus, S. thermophilus） toward two pathogenic bacteria （S. aureus and E. colO. In addition, we evaluate the influence of this fiber either on the localization of the lactic inhibiting substances and their extracelluler polymer substances （EPS） production. The results showed that the prebiotic candidate noticeably elongated the adaptation phase of the target pathogens to up than 12 hours, while stimulate the probiotic growth till 2 Log CFU/mL in favor of L. rhamnosus and to approximately 3 Log CFU/mL for S. thermophilus compared with control substrate （glucose）. The inhibiting effect was more important toward S. aureus （2.54 and 1.09 Log CFU/mL against 4.99 and 7.75 in co-culture with L. rhamnosus and S. thermophilus respectively at the 4th hour of incubation） and lesser for E. coli compared with the control substrate. And the results suggested that S thermophilus inhibited both target strains mainly by bacteriocins, whilst L. rharnnosus rather inhibited S. aureus growth by bacteriocins and E. coli ones by acids production. We also observed a significant stimulation of the exopolysaccahrids production by this fiber, which may allow a better adhesion of the probiotic candidates in the intestinal tract.
