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双外标校准高效液相色谱法同时测定中药牛膝中皂苷类成分和甾酮类成分 被引量:3
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作者 吴冰筱 金武燮 +4 位作者 张留记 谷丽华 李林楠 杨莉 王峥涛 《药学学报》 CAS CSCD 北大核心 2022年第6期1868-1873,共6页
牛膝现行的质量标准难以有效控制其质量。本研究运用双外标结合校正因子,同时测定牛膝中牛膝皂苷C、牛膝皂苷D、β-蜕皮甾酮、25R-牛膝甾酮和25S-牛膝甾酮的含量。采用Agilent Poroshell 120 EC-C18(150 mm×4.6 mm,2.7μm)色谱柱,... 牛膝现行的质量标准难以有效控制其质量。本研究运用双外标结合校正因子,同时测定牛膝中牛膝皂苷C、牛膝皂苷D、β-蜕皮甾酮、25R-牛膝甾酮和25S-牛膝甾酮的含量。采用Agilent Poroshell 120 EC-C18(150 mm×4.6 mm,2.7μm)色谱柱,流动相为0.1%磷酸乙腈-0.1%磷酸水溶液梯度洗脱,流速0.8 mL·min^(-1),柱温35℃,进样量5μL,分析时间30 min。分别以β-蜕皮甾酮和牛膝皂苷D为对照,计算25R-牛膝甾酮、25S-牛膝甾酮和牛膝皂苷C的相对校正因子(relative correction factors,RCF)和相对保留时间(relative retention time,RRT),25R-牛膝甾酮、25S-牛膝甾酮和牛膝皂苷C的相对校正因子分别为1.116、1.056和0.8881,不同条件下RCF稳定性良好。分别使用外标法和双外标校准法对20批不同来源牛膝样品的含量进行计算,两种方法所得结果相对误差(relative error,RE)小于5%,表明建立的双外标校准法同时测定牛膝中5种成分准确可行。含量测定结果显示牛膝2种皂苷和3种甾酮的含量分别为0.597%~1.916%和0.044%~0.150%,皂苷含量约为甾酮的10倍,提示牛膝皂苷是牛膝药材质量的关键成分。该研究结果为牛膝药材提供了科学可行的整体质量评价方法。 展开更多
关键词 牛膝 牛膝皂苷D β-蜕皮甾酮 外标校准 高效液相色谱 质量评价
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LA-ICP-MS法分析粉煤灰中的主要和微量元素分布
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作者 王炜 《环境保护与循环经济》 2014年第6期56-59,共4页
利用激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)来分析单个的粉煤灰,以达到确定灰分颗粒的性质以及微量元素的附着情况。根据粉煤灰分类方式,外标校准和内标标准化两种分析方法会被应用到两个灰分样品的分析当中。
关键词 飞灰颗粒 外标校准 内标标准化 重金属和元素分布
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A Target-Orientated Marker Image Binarization Method for Orthopaedic Surgical Navigation System 被引量:1
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作者 闫士举 陈晓军 +2 位作者 王成焘 苏颖颖 夏庆 《Journal of Shanghai Jiaotong university(Science)》 EI 2007年第1期18-22,共5页
Camera calibration is the key technique in a C-arm based orthopaedic surgical navigation system. The extraction of marker location information is a necessary step in the calibration process. Ideal marker images should... Camera calibration is the key technique in a C-arm based orthopaedic surgical navigation system. The extraction of marker location information is a necessary step in the calibration process. Ideal marker images should possess uniform background and contain marker shadow only, but in fact marker images always possess nonuniform background and are contaminated by noise and unwanted anatomic information, making the extraction very difficult. A target-orientated marker shadow extraction method was proposed. With this method a proper threshold for marker image binarization can be determined. 展开更多
关键词 C-ARM orthopaedic surgery marker image BINARIZATION gray-scale threshold
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Infrared radiation measurement of the aerial target based on temperature calibration and target images 被引量:2
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作者 CAO Xi-zheng GUO Li-hong LI Zhuo 《Optoelectronics Letters》 EI 2006年第6期463-467,共5页
The gray level values of target pixels of infrared images processed by the SUSAN noise filtering algorithm and the background filtering algorithm are calculated,assuming that the target distance is given in image acqu... The gray level values of target pixels of infrared images processed by the SUSAN noise filtering algorithm and the background filtering algorithm are calculated,assuming that the target distance is given in image acquisition.The equivalent blackbody temperature is obtained by the temperature calibration model that represents the quantitative relation,at a certain integration time,between the gray level value and the equivalent blackbody temperature.The temperature calibration model has been acquired by the curve fitting with the experimental data of blackbody temperature calibration using Origin 6.0.Then the target radiance can be determined by the radiant flux equivalence model built on condition that the target radiant flux received by the infrared detector is equivalent to that of the blackbody placed in front of the infrared detector at a close distance.This measuring method is based on the temperature calibration parameters and real image information,so it can avoid subjectivity of the traditional theory modeling methods. 展开更多
关键词 红外线辐射测量 温度校准 目标图像 滤波算法
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