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大麦种子前期处理对其外植体性状的调控和对其机制的探讨(Ⅰ) 被引量:1
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作者 邵宏波 梁海曼 《广西植物》 CAS CSCD 北大核心 2001年第4期347-352,共6页
研究了种壳对大麦种子吸胀生理影响的调节作用 ,同时对其外植体性状的调控作用和其机制也进行了探讨。结果如下 :(1 )种壳显著延缓种子的吸水进程 ;(2 )种壳对分子渗漏影响显著 ;(3 )总 N的渗漏主要来自胚乳 ,Mn+ + 的渗漏在胚中相对较... 研究了种壳对大麦种子吸胀生理影响的调节作用 ,同时对其外植体性状的调控作用和其机制也进行了探讨。结果如下 :(1 )种壳显著延缓种子的吸水进程 ;(2 )种壳对分子渗漏影响显著 ;(3 )总 N的渗漏主要来自胚乳 ,Mn+ + 的渗漏在胚中相对较多 ,种壳对 K+ 渗漏影响较大 ;(4 )去壳促进吸胀期间的可溶性蛋白量和过氧化物酶同工酶活性上升 ;(5 )吸胀使 IAA、GA3含量上升 ,使胚乳中的 ABA含量显著减少 ,去壳显著促进吸胀初期胚乳中的 ABA、IAA、GA3含量上升 ,去壳减少吸胀后期胚中的 IAA、ABA、GA3含量 ;(6)去壳减少吸胀 2 4h并且种子进行培养时的培养效果 ,它们具有明显较高的萌发率和愈伤组织诱导率。 展开更多
关键词 大麦种子 前期处理 吸胀 种壳 渗漏 矿质元素 内源激素 愈伤组织 外植体性状 调控
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AN EXPERIMENTAL STUDY OF THE TUMOUR NECROSIS FACTOR LEVELSIN AQUEOUS HUMOR AFTER TRAUMATIC CATARACT AND INTRAOCULAR LENS IMPLANTATION 被引量:2
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作者 周朝晖 何守志 《Chinese Medical Sciences Journal》 CAS CSCD 1999年第1期64-66,共3页
Purpose. This paper studies the tumour necrosis factor (TNF) levels in aqueous humor after traumatic cataract extraction and posterior chamber (PC) intraocular lens (IOL) implantation in rabb... Purpose. This paper studies the tumour necrosis factor (TNF) levels in aqueous humor after traumatic cataract extraction and posterior chamber (PC) intraocular lens (IOL) implantation in rabbits,and discusses the effect of TNF on postoperative anterior ocular inflammation. Methods. Twenty seven pigmented rabbits were divided into three groups: for the first group, the IOL were placed in the capsular bag after traumatic cataract extraction; for the second, the Extracapsular cataract extraction without IOL implantation; and for the third, the control group without surgical intervention. On the 1st, 3rd, 7th and 14th day postoperatively, aqueous humor samples were obtained. A modified double antibodies indirect sandwich ELISA was used to detected for the presence of TNF. The data were studied by means of analysis of variance in SAS software. Result. The TNF level was increased in aqueous humor and reached its maximum on the 1st postoperative days after traumatic cataract extraction and PC IOLs implantation, and the TNF levels are significantly higher (P<0 05) on the 1st, 3rd, 7th and 14th day postoperatively in traumatic cataract extraction and PC IOL implanted group than that in the non surgical intervention group and extracapsular cataract extraction group. Conclusions. The increase of TNF levels had a close relationship with presence of the IOL itself which induces the secretion of TNF. This suggested that TNF as the principal mediators of immunological and inflammatory responses, so that may play critical role in anterior ocular inflammative response after traumatic cataract extraction and IOL implantation. 展开更多
关键词 intraocular lens traumatic cataract extraction tumour necrosis factor (TNF)
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