[Objective] The research aimed to establish the fast and efficient rapid propagation system for Ampelopsis grossedentata by using the tissue culture method. [Method] Ampelopsis grossedentata stem with the bud was the ...[Objective] The research aimed to establish the fast and efficient rapid propagation system for Ampelopsis grossedentata by using the tissue culture method. [Method] Ampelopsis grossedentata stem with the bud was the material,and the influences of different sterilization methods,media and hormone combinations on the in vitro rapid propagation of Ampelopsis grossedentata were studied. [Result] The explant sterilization effect of 75% ethanol dipping 20 s ＋ 0.1% HgCl2 treating 8 min ＋ Tween-80 1-2 drops was better. The optimal medium for the axillary bud induction was B5 ＋ 1.00 mg/L BA ＋ 0.05 mg/L NAA. The optimal multiplication medium was MS/B5 ＋ 1.50 mg/L BA ＋ 0.05 mg/L NAA. 1/2MS ＋ 0.50 mg/L IBA was the best medium for the rooting. [Conclusion] The high frequency occurrence system of Ampelopsis grossedentata in vitro rapid propagation was established. It laid the technology basis for the callus regeneration system,genetic transformation and clonal mutation screening.展开更多
Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different c...Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA ＋ NAA or BA ＋ Kin. BA at concentration （2 mg L-1 BA） gave the best results in percentage response and number of shoots/plant （100%, 2.84） respectively. While, Kin at 3 mg L-1 gave the highest average shoot length （2.81 cm）. However, MS medium supplemented with 2.5 mg L-1 ＋ 0.5 mg L-1 Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants （3.7, 8.2） respectively. While the highest length of shoot （4.7 cm） in MS medium supplemented with 3 mg L-1 Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg L-1 IBA （Indole-3-butyric acid） was obtained.展开更多
基金Supported by Ministry of Agriculture Major Technology Research Special Item of Agricultural Structure Adjustment (06-08-02B)~~
文摘[Objective] The research aimed to establish the fast and efficient rapid propagation system for Ampelopsis grossedentata by using the tissue culture method. [Method] Ampelopsis grossedentata stem with the bud was the material,and the influences of different sterilization methods,media and hormone combinations on the in vitro rapid propagation of Ampelopsis grossedentata were studied. [Result] The explant sterilization effect of 75% ethanol dipping 20 s ＋ 0.1% HgCl2 treating 8 min ＋ Tween-80 1-2 drops was better. The optimal medium for the axillary bud induction was B5 ＋ 1.00 mg/L BA ＋ 0.05 mg/L NAA. The optimal multiplication medium was MS/B5 ＋ 1.50 mg/L BA ＋ 0.05 mg/L NAA. 1/2MS ＋ 0.50 mg/L IBA was the best medium for the rooting. [Conclusion] The high frequency occurrence system of Ampelopsis grossedentata in vitro rapid propagation was established. It laid the technology basis for the callus regeneration system,genetic transformation and clonal mutation screening.
文摘Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA ＋ NAA or BA ＋ Kin. BA at concentration （2 mg L-1 BA） gave the best results in percentage response and number of shoots/plant （100%, 2.84） respectively. While, Kin at 3 mg L-1 gave the highest average shoot length （2.81 cm）. However, MS medium supplemented with 2.5 mg L-1 ＋ 0.5 mg L-1 Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants （3.7, 8.2） respectively. While the highest length of shoot （4.7 cm） in MS medium supplemented with 3 mg L-1 Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg L-1 IBA （Indole-3-butyric acid） was obtained.
