In our laboratory, FISH technology has been applied in the detection of alien chromosome or large alien chromosome fragments (for instance, the rye chromosome fragments in translocation line of wheat). But for investi...In our laboratory, FISH technology has been applied in the detection of alien chromosome or large alien chromosome fragments (for instance, the rye chromosome fragments in translocation line of wheat). But for investigating translocations involving small segment of alien chromosomes, DNA fragment analysis based technologies such as RAPD and AFLP have been used. The latter methods also have been applied to pbtain molecular markers linked to specific genes (our focus was the Rf3 restorer gene of G type CMS of wheat). An alternative method for finding new molecular markers is subtractive hybridization. Our aim in using this method is to obtain high copy sequences, which are specific to rye.展开更多
文摘In our laboratory, FISH technology has been applied in the detection of alien chromosome or large alien chromosome fragments (for instance, the rye chromosome fragments in translocation line of wheat). But for investigating translocations involving small segment of alien chromosomes, DNA fragment analysis based technologies such as RAPD and AFLP have been used. The latter methods also have been applied to pbtain molecular markers linked to specific genes (our focus was the Rf3 restorer gene of G type CMS of wheat). An alternative method for finding new molecular markers is subtractive hybridization. Our aim in using this method is to obtain high copy sequences, which are specific to rye.
