The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were selfcrossed and the foreign auxin genes in pla...The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were selfcrossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leaf y head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs nd for the breeding of new varietywith rapid growth trait.展开更多
This paper introduces the discovery,composition and structure of oxalate oxidase,as well as illustrates the biological functions of this enzyme.With a comprehensive introduction upon previous researches upon gene clon...This paper introduces the discovery,composition and structure of oxalate oxidase,as well as illustrates the biological functions of this enzyme.With a comprehensive introduction upon previous researches upon gene cloning and heredity transformation of this enzyme,it indicates that heredity transformation can increase the content of oxalate oxidase within the plants and also enhance their resistance.The paper also points out the problems such as lack of gene resources and difficulty in the transformation of heterologous genes,and the focus in later researches should be laid upon the exploration of plant resources relative to this enzyme and selection of resistant species.展开更多
In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector...In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector strategy,we first inserted the remolded tPA gene into the Ti-derived plasmid, PGA643 and then transferred,in a triparental mating system,the plasmid into a strain of Agrobacterium tumefaciens,A281, that contained vir-DNA.Finally poplar cells were transformed with A. tumefaciens using leaf discus method. The transformed cells were selected in culture containing kanamycin and in siyu hybridization.Successful generation of transgenic poplar was demonstrated by molecular hybridization in the cultivated plants and detection of tPA gene product.展开更多
In plants, post-transcriptional gene silencing(PTGS) protects the genome from foreign genes and restricts the expression of certain endogenous genes for proper development. Here, we review the recent progress about ho...In plants, post-transcriptional gene silencing(PTGS) protects the genome from foreign genes and restricts the expression of certain endogenous genes for proper development. Here, we review the recent progress about how the unwanted PTGS is avoided in plants. As a decision-making step of PTGS, aberrant transcripts from most endogenous coding genes are strictly sorted to the bidirectional RNA decay pathways in cytoplasm but not to the short interference RNA(si RNA)-mediated PTGS, with the exception of a few development-relevant endogenous si RNA-producing genes. We also discuss a finely balanced PTGS threshold model that plants fully take advantage of the power of PTGS without self-harm.展开更多
文摘The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were selfcrossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leaf y head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs nd for the breeding of new varietywith rapid growth trait.
基金Supported by Jilin Province Post-doctoral Project"Exploration of Quality Bean Sclerotiniose-tolerant Seeds and Genetic Resources"(00206)
文摘This paper introduces the discovery,composition and structure of oxalate oxidase,as well as illustrates the biological functions of this enzyme.With a comprehensive introduction upon previous researches upon gene cloning and heredity transformation of this enzyme,it indicates that heredity transformation can increase the content of oxalate oxidase within the plants and also enhance their resistance.The paper also points out the problems such as lack of gene resources and difficulty in the transformation of heterologous genes,and the focus in later researches should be laid upon the exploration of plant resources relative to this enzyme and selection of resistant species.
文摘In this study, tPA gene(human tissuetype plasminogen activator gene) was remolded with the leader sequence removed and an artificially synthesized sequence containing the initiator (ATG) added. Using the binary vector strategy,we first inserted the remolded tPA gene into the Ti-derived plasmid, PGA643 and then transferred,in a triparental mating system,the plasmid into a strain of Agrobacterium tumefaciens,A281, that contained vir-DNA.Finally poplar cells were transformed with A. tumefaciens using leaf discus method. The transformed cells were selected in culture containing kanamycin and in siyu hybridization.Successful generation of transgenic poplar was demonstrated by molecular hybridization in the cultivated plants and detection of tPA gene product.
基金supported by the National Basic Research Program of China(2012CB910902)the National Natural Science Foundation of China(9121730591017010)to H.G
文摘In plants, post-transcriptional gene silencing(PTGS) protects the genome from foreign genes and restricts the expression of certain endogenous genes for proper development. Here, we review the recent progress about how the unwanted PTGS is avoided in plants. As a decision-making step of PTGS, aberrant transcripts from most endogenous coding genes are strictly sorted to the bidirectional RNA decay pathways in cytoplasm but not to the short interference RNA(si RNA)-mediated PTGS, with the exception of a few development-relevant endogenous si RNA-producing genes. We also discuss a finely balanced PTGS threshold model that plants fully take advantage of the power of PTGS without self-harm.
