目的观察大翅蓟属植物提取物对皮肤屏障相关蛋白的表达的影响,探讨其屏障修复作用。方法通过使用胶带黏贴的方法,对离体培养的皮肤造成皮肤屏障功能损伤模型,每隔2 d涂抹大翅蓟属植物提取物,7 d后评估损伤皮肤的淋巴上皮Kazal型相关抑...目的观察大翅蓟属植物提取物对皮肤屏障相关蛋白的表达的影响,探讨其屏障修复作用。方法通过使用胶带黏贴的方法,对离体培养的皮肤造成皮肤屏障功能损伤模型,每隔2 d涂抹大翅蓟属植物提取物,7 d后评估损伤皮肤的淋巴上皮Kazal型相关抑制因子(Lympho-epithelial Kazal type related inhibitor,LEKTI),兜甲蛋白(Loricrin,LOR),内披蛋白(Involucrin,INV)丝聚合蛋白(Filaggrin,FLG)的表达情况。结果正常皮肤和受损皮肤的LEKTI,LOR,INV,FLG染色均有增强,受损皮肤增强更为明显。结论大翅蓟属植物提取物可以提高皮肤FLG、LOR、INV、LEKTI的表达,进而提高皮肤屏障功能。展开更多
Causative gene defects have not been demonstrated in the majority of nonbullous congenital ichthyosiform erythroderma (NBCIE) cases. The purpose of this study was to further elucidate the pathogenesis of NBCIE. Immuno...Causative gene defects have not been demonstrated in the majority of nonbullous congenital ichthyosiform erythroderma (NBCIE) cases. The purpose of this study was to further elucidate the pathogenesis of NBCIE. Immunohistochemical and ultrastructural observations, transglutaminase activity assays and sequencing of TGM1 were performed in five patients from four NBCIE families. Transglutaminase 1 (TGase 1), involucrin and loricrin expression and in situ transglutaminase activity were present in all of the cases. Ultrastructurally, two cases out of five showed incomplete thickening of the cornified cell envelope (CCE) during keratinization and the other three exhibited abnormal lipid droplets in the cornified cells and malformed lamellar granules. No TGM1 mutation was found in any of the four families by direct sequence analysis. NBCIE cases with normal TGase 1 seemed to have two distinct patterns of abnormality, one with abnormal lipid droplets and malformed lamellar granules and the other with defective CCE formation.展开更多
文摘目的观察大翅蓟属植物提取物对皮肤屏障相关蛋白的表达的影响,探讨其屏障修复作用。方法通过使用胶带黏贴的方法,对离体培养的皮肤造成皮肤屏障功能损伤模型,每隔2 d涂抹大翅蓟属植物提取物,7 d后评估损伤皮肤的淋巴上皮Kazal型相关抑制因子(Lympho-epithelial Kazal type related inhibitor,LEKTI),兜甲蛋白(Loricrin,LOR),内披蛋白(Involucrin,INV)丝聚合蛋白(Filaggrin,FLG)的表达情况。结果正常皮肤和受损皮肤的LEKTI,LOR,INV,FLG染色均有增强,受损皮肤增强更为明显。结论大翅蓟属植物提取物可以提高皮肤FLG、LOR、INV、LEKTI的表达,进而提高皮肤屏障功能。
文摘Causative gene defects have not been demonstrated in the majority of nonbullous congenital ichthyosiform erythroderma (NBCIE) cases. The purpose of this study was to further elucidate the pathogenesis of NBCIE. Immunohistochemical and ultrastructural observations, transglutaminase activity assays and sequencing of TGM1 were performed in five patients from four NBCIE families. Transglutaminase 1 (TGase 1), involucrin and loricrin expression and in situ transglutaminase activity were present in all of the cases. Ultrastructurally, two cases out of five showed incomplete thickening of the cornified cell envelope (CCE) during keratinization and the other three exhibited abnormal lipid droplets in the cornified cells and malformed lamellar granules. No TGM1 mutation was found in any of the four families by direct sequence analysis. NBCIE cases with normal TGase 1 seemed to have two distinct patterns of abnormality, one with abnormal lipid droplets and malformed lamellar granules and the other with defective CCE formation.