A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the rea...A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear membrane, nuclear pore complexes, and decondensed chromatin etc. Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei. Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only eggderived lamin LIII.展开更多
On the basis of choosing the basic element as the bar and choosing the basic mesh as the triangle as well as supposing the conditions of the element, the membrane states of an antenna reflector were researched by the ...On the basis of choosing the basic element as the bar and choosing the basic mesh as the triangle as well as supposing the conditions of the element, the membrane states of an antenna reflector were researched by the analogue method, because the membrane effect was not omitted during the ending deployment process of the radial rib antenna. The expressions of the bar element’s section area and density were obtained, while the expression of the stress state during the ending deployment process of antenna was attained. During the establishment process of the analogue method, the analysis method of the net shell structure was employed. Moreover, during the backward deduction of membrane stress, the continuation method was adopted. Because the expression of the membrane stress state can realize the analysis on the antenna membrane state, this research has great significance of theoretical direction to the normal operation of the space deployable antenna.展开更多
The electroluminescence thin films doped with erbium, fabricated by thermal evaporation with two boats, are analyzed by X-ray diffraction (XRD). The relationship between electroluminescence brightness and microstructu...The electroluminescence thin films doped with erbium, fabricated by thermal evaporation with two boats, are analyzed by X-ray diffraction (XRD). The relationship between electroluminescence brightness and microstructure of the thin films is obtained. The results reveal that the large grain size in high index plane of deposited microcrystalline film has an effect on electroluminescence characteristics of the film devices.展开更多
Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a s...Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a signal peptide and C-terminal intracellular kinase. Researches in the last two decades have uncovered an increasing number of RLKs that regulate plant development, stress response and sexual reproduction, highlighting a dominant role of RLK signaling in cell-to-cell communications. Sexual reproduction in flowering plants is featured by interactions between the male gametophyte and the female tissues to facilitate sperm delivery and fertilization. Emerging evidences suggest that RLKs regulate almost every aspect of plant reproductive process, especially during pollination. Therefore, in this review we will focus mainly on the function and signaling of RLKs in plant male-female interaction and discuss the future prospects on these topics.展开更多
Plant receptor-like protein kinases(RLKs) are transmembrane proteins with an extracellular domain and an intracellular kinase domain, which enable plant perceiving diverse extracellular stimuli to trigger the intracel...Plant receptor-like protein kinases(RLKs) are transmembrane proteins with an extracellular domain and an intracellular kinase domain, which enable plant perceiving diverse extracellular stimuli to trigger the intracellular signal transduction. The somatic embryogenesis receptor kinases(SERKs) code the leucine-rich-repeat receptor-like kinase(LRR-RLK), and have been demonstrated to associate with multiple ligand-binding receptors to regulate plant growth, root development, male fertility, stomatal development and movement, and immune responses. Here, we focus on the progress made in recent years in understanding the versatile functions of Arabidopsis SERK proteins, and review SERK proteins as co-receptor to perceive different endogenous and environmental cues in different signaling pathway, and discuss how the kinase activity of SERKs is regulated by various modification.展开更多
In a seminal work, Gozar et al. reported on the high-temperature interface superconductivity in bilayers of insulating La2Cu O4 and metallic La2-xSrxCuO4(x=0.45). An interesting question to address is how general and ...In a seminal work, Gozar et al. reported on the high-temperature interface superconductivity in bilayers of insulating La2Cu O4 and metallic La2-xSrxCuO4(x=0.45). An interesting question to address is how general and robust this interface superconductivity is. In the past, the cuprate bilayers were grown in a unique atomic-layer molecular beam epitaxy system, with a Sr doping range of x≤0.47, and the atomically flat interface was thought to be indispensable. Here, we have fabricated bilayers of La2CuO4 and La2-xSrxCuO4 by pulsed laser deposition. We have tried to extend the nominal doping range of Sr from the previous maximum of 0.47 to the present1.70(the nominal Sr content in the targets). X-ray diffraction result indicates that our La2-xSrxCuO4 films with x≤0.60 have very high crystalline quality;but the film crystalline structure degrades gradually with further increasing x, and finally the structure is fully lost when x reaches 1.40 and higher. Although the film quality scatters dramatically, our experiments show that there exists superconductivity for bilayers in nearly the entire over-doped Sr range, except for a non-superconducting region at x^0.80. These observations demonstrate that the interface superconductivity in copper oxides is very general and robust.展开更多
文摘A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear membrane, nuclear pore complexes, and decondensed chromatin etc. Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei. Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only eggderived lamin LIII.
基金Sponsored by the National Natural Science Foundation of China(Grant No.19682003)
文摘On the basis of choosing the basic element as the bar and choosing the basic mesh as the triangle as well as supposing the conditions of the element, the membrane states of an antenna reflector were researched by the analogue method, because the membrane effect was not omitted during the ending deployment process of the radial rib antenna. The expressions of the bar element’s section area and density were obtained, while the expression of the stress state during the ending deployment process of antenna was attained. During the establishment process of the analogue method, the analysis method of the net shell structure was employed. Moreover, during the backward deduction of membrane stress, the continuation method was adopted. Because the expression of the membrane stress state can realize the analysis on the antenna membrane state, this research has great significance of theoretical direction to the normal operation of the space deployable antenna.
文摘The electroluminescence thin films doped with erbium, fabricated by thermal evaporation with two boats, are analyzed by X-ray diffraction (XRD). The relationship between electroluminescence brightness and microstructure of the thin films is obtained. The results reveal that the large grain size in high index plane of deposited microcrystalline film has an effect on electroluminescence characteristics of the film devices.
基金supported by the Ministry of Science and Technology of China(2013CB945103)the National Natural Science Foundation of China(31330053)
文摘Different from animals, sessile plants are equipped with a large receptor-like kinase(RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a signal peptide and C-terminal intracellular kinase. Researches in the last two decades have uncovered an increasing number of RLKs that regulate plant development, stress response and sexual reproduction, highlighting a dominant role of RLK signaling in cell-to-cell communications. Sexual reproduction in flowering plants is featured by interactions between the male gametophyte and the female tissues to facilitate sperm delivery and fertilization. Emerging evidences suggest that RLKs regulate almost every aspect of plant reproductive process, especially during pollination. Therefore, in this review we will focus mainly on the function and signaling of RLKs in plant male-female interaction and discuss the future prospects on these topics.
基金supported by the National Natural Science Foundation of China(31470376)the"Qilu Scholarship"from Shandong University(11200083963009)"1000-talents Plan"from China for young researchers(11200084963002)
文摘Plant receptor-like protein kinases(RLKs) are transmembrane proteins with an extracellular domain and an intracellular kinase domain, which enable plant perceiving diverse extracellular stimuli to trigger the intracellular signal transduction. The somatic embryogenesis receptor kinases(SERKs) code the leucine-rich-repeat receptor-like kinase(LRR-RLK), and have been demonstrated to associate with multiple ligand-binding receptors to regulate plant growth, root development, male fertility, stomatal development and movement, and immune responses. Here, we focus on the progress made in recent years in understanding the versatile functions of Arabidopsis SERK proteins, and review SERK proteins as co-receptor to perceive different endogenous and environmental cues in different signaling pathway, and discuss how the kinase activity of SERKs is regulated by various modification.
基金supported by the National Key Research and Development Program of Ministry of Science and Technology of China (2017YFA0303002, 2016YFA0300204, and 2016YFA0300701)the Fundamental Research Funds for the Central Universities
文摘In a seminal work, Gozar et al. reported on the high-temperature interface superconductivity in bilayers of insulating La2Cu O4 and metallic La2-xSrxCuO4(x=0.45). An interesting question to address is how general and robust this interface superconductivity is. In the past, the cuprate bilayers were grown in a unique atomic-layer molecular beam epitaxy system, with a Sr doping range of x≤0.47, and the atomically flat interface was thought to be indispensable. Here, we have fabricated bilayers of La2CuO4 and La2-xSrxCuO4 by pulsed laser deposition. We have tried to extend the nominal doping range of Sr from the previous maximum of 0.47 to the present1.70(the nominal Sr content in the targets). X-ray diffraction result indicates that our La2-xSrxCuO4 films with x≤0.60 have very high crystalline quality;but the film crystalline structure degrades gradually with further increasing x, and finally the structure is fully lost when x reaches 1.40 and higher. Although the film quality scatters dramatically, our experiments show that there exists superconductivity for bilayers in nearly the entire over-doped Sr range, except for a non-superconducting region at x^0.80. These observations demonstrate that the interface superconductivity in copper oxides is very general and robust.
