This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering sca...This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture.展开更多
基金National High-tech Reasearch and Development Program of China(863 Program)grant number:2077AA09Z436+1 种基金Guangdong Project '211'grant number:50621030
文摘This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture.
