To analyze a multibody system composed of non-uniform beam and spring-mass subsystems, the model discretization is carried on by utilizing the finite element method(FEM), the dynamic model of non-uniform beam is dev...To analyze a multibody system composed of non-uniform beam and spring-mass subsystems, the model discretization is carried on by utilizing the finite element method(FEM), the dynamic model of non-uniform beam is developed by using the transfer matrix method of multibody system(MS-TMM), the transfer matrix of non-u- niform beam is derived, and the natural frequencies are computed. Compared with the numerical assembly method (NAM), the results by MS-TMM have good agreement with the results by FEM, and are better than the results by NAM. When using the high precision method, the global dynamic equations of the complex multibody system are not needed and the orders of involved system matrices are decreased greatly. For the investigation on the re- verse problem of the physical parameter identification of multibody system, MS-TMM and the optimization tech- nology based on genetic algorithms(GAs) are combined and extended. The identification problem is exchanged for an optimization problem, and it is formulated as a global minimum solution of the objective function with respect to natural frequencies of multibody system. At last, the numerical example of non-uniform beam with attach- ments is discussed, and the identification results indicate the feasibility and the effectivity of the proposed aop- proach.展开更多
The ethanolic extracts of 100 common traditional Chinese drugs, which are widely used in many prescriptions in treatment of cancer in China, were screened for MDR of KBV200 cell line in vitro with MTT method. The ...The ethanolic extracts of 100 common traditional Chinese drugs, which are widely used in many prescriptions in treatment of cancer in China, were screened for MDR of KBV200 cell line in vitro with MTT method. The result showed 9 extracts having MDR reversal activity. They were the extracts of Fructus Lagenariae Sicerariae, Radix Glycyrrhizae, Poria, Herba Andrographitis, Radix Sophorae Tonkinensis, Caulis Mahoniae, Folium Artemisiae Argyi, Rhizoma Curcumae, Fructus Cnidii. Other 5 extracts showed cytotoxic on KBV200 cell line. $$$$展开更多
A proof is given that any λ _polynome over real quaternionic sfield can be factorized into produce of some linear factors.By the way,some properties and applications of this factorization in matrix theory are given.
Based on the constructivism, using the College English(new edition) CD-ROMs for classroom teaching as main media, this paper explores the feasibility of the classroom teaching mode of appropriate combination of trad...Based on the constructivism, using the College English(new edition) CD-ROMs for classroom teaching as main media, this paper explores the feasibility of the classroom teaching mode of appropriate combination of traditional methods with the multimedia overhead projector. The new teaching mode proves to be successful in that it has greatly improved the students' ability to use English in an all-round way and has obvious advantages over the only traditional method.展开更多
Numerical simulations are performed on the interface with large deformation induced by the interaction between a moving shock and two consecutive bubbles. The high performance of the level set method for multi-materia...Numerical simulations are performed on the interface with large deformation induced by the interaction between a moving shock and two consecutive bubbles. The high performance of the level set method for multi-material interfaces is demonstrated. Discontinuous Galerkin finite element method is used to solve Euleri- an equations. And the fifth-order weighted essentially non-oscillatory (WENO) scheme is used to solve the level set equation for capturing multi-material interfaces. The ghost fluid method is used to deal with the interfacial boundary condition. Results are obtained for two bubble interacting with a moving shock. The contours of the constant density and the pressure at different time are given. In the computational domain, three different cases are considered, i.e. two helium bubbles, a helium bubble followed by an R22 bubble in the direction of the moving shock, and an R22 bubble followed by a helium bubble. Computational results indicate that multi-mate- rial interfaces can be properly captured by the level set method. Therefore, for problems involving the flow of three different materials with two different interfaces, each interface separating two different materials can be similarly handled.展开更多
A forward recursive formulation based on corotational frame is proposed for flexible planar beams with large displacement.The traditional recursive formulation has been successfully used for flexible mutibody dynamics...A forward recursive formulation based on corotational frame is proposed for flexible planar beams with large displacement.The traditional recursive formulation has been successfully used for flexible mutibody dynamics to improve the computational efficiency based on floating frame,in which the assumption of small strain and deflection is adopted.The proposed recursive formulation could be used for large displacement problems based on the corotational frame.It means that the recursive scheme is used not only for adjacent bodies but also for adjacent beam elements.The nodal relative rotation coordinates of the planar beam are used to obtain equations with minimal generalized coordinates in present formulation.The proposed formulation is different from absolute nodal coordinate formulation and the geometrically exact beam formulation in which the absolute coordinates are used.The recursive scheme and minimal set of dynamic equations lead to a high computational efficiency in numerical integration.Numerical examples are carried out to demonstrate the accuracy and validity of this formulation.For all of the examples,the results of the present formulation are in good agreement with results obtained using commercial software and the published results.Moreover,it is shown that the present formulation is more efficient than the formulation in ANSYS based on GEBF.展开更多
The discrete particle method was used to simulate the distribution of gas holdup in a gas-liquid standard Rushton stirred tank. The gas phase was treated as a large number of bubbles and their trajectories were tracke...The discrete particle method was used to simulate the distribution of gas holdup in a gas-liquid standard Rushton stirred tank. The gas phase was treated as a large number of bubbles and their trajectories were tracked with the results of motion equations. The two-way approach was performed to couple the interphase momentum exchange. The turbulent dispersion of bubbles with a size distribution was modeled using a stochastic tracking model, and the added mass force was involved to account for the effect of bubble acceleration on the surrounding fluid. The predicted gas holdup distribution showed that this method could give reasonable prediction comparable to the reported experimental data when the effect of turbulence was took into account in modification for drag coefficient.展开更多
A decentralized task planning algorithm is proposed for heterogeneous unmanned aerial vehicle(UAV)swarm with different capabilities.The algorithm extends the consensus-based bundle algorithm(CBBA)to account for a more...A decentralized task planning algorithm is proposed for heterogeneous unmanned aerial vehicle(UAV)swarm with different capabilities.The algorithm extends the consensus-based bundle algorithm(CBBA)to account for a more realistic and complex environment.The extension of the algorithm includes handling multi-agent task that requires multiple UAVs collaboratively completed in coordination,and consideration of avoiding obstacles in task scenarios.We propose a new consensus algorithm to solve the multi-agent task allocation problem and use the Dubins algorithm to design feasible paths for UAVs to avoid obstacles and consider motion constraints.Experimental results show that the CBBA extension algorithm can converge to a conflict-free and feasible solution for multi-agent task planning problems.展开更多
Mutual information is widely used in medical image registration, because it does not require preprocessing the image. However, the local maximum problem in the registration is insurmountable. We combine mutual informa...Mutual information is widely used in medical image registration, because it does not require preprocessing the image. However, the local maximum problem in the registration is insurmountable. We combine mutual information and gradient information to solve this problem and apply it to the non-rigid deformation image registration. To improve the accuracy, we provide some implemental issues, for example, the Powell searching algorithm, gray interpolation and consideration of outlier points. The experimental results show the accuracy of the method and the feasibility in non-rigid medical image registration.展开更多
GIS not includes only spatial data and attribute data, but also multimedia information. How to integrate and manage these data effectively is very important. With the development of the technology of Object-Relation D...GIS not includes only spatial data and attribute data, but also multimedia information. How to integrate and manage these data effectively is very important. With the development of the technology of Object-Relation DBMS (ORDBMS), it improved the capability of managing complicated information. With ORDBMS, GIS and multimedia information can be integrated together and can speed accessing files by using "buffer".展开更多
This study establishes the launch dynamics method,sensitivity analysis method,and multiobjective dynamic optimization method for the dynamic simulation analysis of the multiple launch rocket system(MLRS)based on the R...This study establishes the launch dynamics method,sensitivity analysis method,and multiobjective dynamic optimization method for the dynamic simulation analysis of the multiple launch rocket system(MLRS)based on the Riccati transfer matrix method for multibody systems(RMSTMM),direct differentiation method(DDM),and genetic algorithm(GA),respectively.Results show that simulation results of the dynamic response agree well with test results.The sensitivity analysis method is highly programming,the matrix order is low,and the calculation time is much shorter than that of the Lagrange method.With the increase of system complexity,the advantage of a high computing speed becomes more evident.Structural parameters that have the greatest influence on the dynamic response include the connection stiffness between the pitching body and the rotating body,the connection stiffness between the rotating body and the vehicle body,and the connection stiffnesses among 14^(#),16^(#),and 17^(#)wheels and the ground,which are the optimization design variables.After optimization,angular velocity variances of the pitching body in the revolving and pitching directions are reduced by 97.84%and 95.22%,respectively.展开更多
An improved model predictive control algorithm is proposed for Hammerstein-Wiener nonlinear systems.The proposed synthesis algorithm contains two parts:offline design the polytopic invariant sets,and online solve the ...An improved model predictive control algorithm is proposed for Hammerstein-Wiener nonlinear systems.The proposed synthesis algorithm contains two parts:offline design the polytopic invariant sets,and online solve the min-max optimization problem.The polytopic invariant set is adopted to replace the traditional ellipsoid invariant set.And the parameter-correlation nonlinear control law is designed to replace the traditional linear control law.Consequently,the terminal region is enlarged and the control effect is improved.Simulation and experiment are used to verify the validity of the wind tunnel flow field control algorithm.展开更多
Unquestionably, anyone born to Urhobo parents, being biologically Urhobo, should speak the Urhobo language. But does everybody born by an Urhobo parent speak Urhobo? From our observation, the answer to this question ...Unquestionably, anyone born to Urhobo parents, being biologically Urhobo, should speak the Urhobo language. But does everybody born by an Urhobo parent speak Urhobo? From our observation, the answer to this question is clearly "no". Many of the Urhobo people in the Diaspora as well as those at home have chosen to shift from their own language to other languages. Hence Urhobo language has gradually acquired the status of a second language. Therefore, its teaching would also veer away from language teaching methods that are used for first languages. Based on Noam Chomsky's theory of Generative Grammar, aspects of the Urhobo grammar will be treated as we present specifically the verb "edia"/"to be". As it is, it is being presented as a second language bearing in mind the great population of Urhobo people in the Diaspora as well as Mowarin's "miguo generation" of Urhobo children back home in Urhobo land. This being so, the methods applied in this study are the Grammar Translation Method Approach as well as the Direct Method Approach. To facilitate its learning especially for the new generation of Urhobo persons, the poetic approach would also be applied.展开更多
In this paper the large deflection dynamic problems of Euler beams are investigated. The vibration control equations are derived based on the multibody system method. A numerical procedure for solving the resulting di...In this paper the large deflection dynamic problems of Euler beams are investigated. The vibration control equations are derived based on the multibody system method. A numerical procedure for solving the resulting differential algebraic equations is presented on the basis of the Newmark direct integration method combined with the Newton-Raphson iterative method. The sub beams are treated as small deformation in the convected coordinate systems, which can greatly simplify the deformation description. The rigid motions of the sub beams are taken into account through the motions of the convected coordinate systems. Numerical ex- amples are carried out, where results show the effectiveness of the proposed method.展开更多
A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized...A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized with sucking-mouse adapted FMD virus (A/AV88(L) strain) were conjugated to colloidal gold beads and used as the capture antibody,and affinity purified polyclonal antibodies from rabbits which were immunized with cell-culture adapted FMD virus (A/CHA/09 strain) were used as detector antibody. On the nitrocellulose membrane of the immunochromatographic strip,the capture antibody was laid on a sample pad,the detector antibody was printed at the test line(T) and goat anti-guinea pigs IgG antibodies were immobilized to the control line(C). The lower detection limit of the test for a FMDV 146S antigen is 11.7ng/ml as determined in serial tests after the strip device was assembled and the assay condition optimization. No cross reactions were found with FMDV serotype C,Swine vesicular disease (SVD),Vesicular stomatiti svirus (VSV) and vesicular exanthema of swine virus (VES) viral antigens with this rapid test. Clinically,the diagnostic sensitivity of this test for FMDV serotypes A was 88.7% which is as same as an indirect-sandwich ELISA. The specificity of this strip test was 98.2% and is comparable to the 98.7% obtained with indirect-sandwich ELISA. This rapid strip test is simple,easy and fast for clinical testing on field sites; no special instruments and skills are required,and the result can be obtained within 15 min. To our knowledge,this is the first rapid immunochromatogarpic assay for serotype A of FMDV.展开更多
Due to the effectiveness, simple deployment and low cost, radio frequency identification (RFID) systems are used in a variety of applications to uniquely identify physical objects. The operation of RFID systems ofte...Due to the effectiveness, simple deployment and low cost, radio frequency identification (RFID) systems are used in a variety of applications to uniquely identify physical objects. The operation of RFID systems often involves a situation in which multiple readers physically located near one another may interfere with one another's operation. Such reader collision must be minimized to avoid the faulty or miss reads. Specifically, scheduling the colliding RFID readers to reduce the total system transaction time or response time is the challenging problem for large-scale RFID network deployment. Therefore, the aim of this work is to use a successful multi-swarm cooperative optimizer called pseo to minimize both the reader-to-reader interference and total system transaction time in RFID reader networks. The main idea of pS20 is to extend the single population PSO to the interacting multi-swarm model by constructing hierarchical interaction topology and enhanced dynamical update equations. As the RFID network scheduling model formulated in this work is a discrete problem, a binary version of PS20 algorithm is proposed. With seven discrete benchmark functions, PS20 is proved to have significantly better performance than the original PSO and a binary genetic algorithm, pS20 is then used for solving the real-world RFID network scheduling problem. Numerical results for four test cases with different scales, ranging from 30 to 200 readers, demonstrate the performance of the proposed methodology.展开更多
Robust video streaming through high error prone wireless channel has attracted much attention. In this paper the authors introduce an effective algorithm by joining the Unequal Error Protection ability of the channel ...Robust video streaming through high error prone wireless channel has attracted much attention. In this paper the authors introduce an effective algorithm by joining the Unequal Error Protection ability of the channel multiplexing protocol H.223 Annex D, and the new H.263++ Annex V Data Partition together. Based on the optimal trade off of these two technologies, the Joint Source and Channel Coding algorithm can result in stronger error resilience. The simulation results have shown its superiority against separate coding mode and some Unequal Error Protection mode under recommended wireless channel error patterns.展开更多
An ancient genome duplication (PPP1) that predates divergence of the cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot dive...An ancient genome duplication (PPP1) that predates divergence of the cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot divergence in the genome of rice (Oryza sativa L.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. Our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice.展开更多
基金Supported by the National Natural Science Foundation of China(10902051)the Natural Science Foundation of Jiangsu Province(BK2008046)~~
文摘To analyze a multibody system composed of non-uniform beam and spring-mass subsystems, the model discretization is carried on by utilizing the finite element method(FEM), the dynamic model of non-uniform beam is developed by using the transfer matrix method of multibody system(MS-TMM), the transfer matrix of non-u- niform beam is derived, and the natural frequencies are computed. Compared with the numerical assembly method (NAM), the results by MS-TMM have good agreement with the results by FEM, and are better than the results by NAM. When using the high precision method, the global dynamic equations of the complex multibody system are not needed and the orders of involved system matrices are decreased greatly. For the investigation on the re- verse problem of the physical parameter identification of multibody system, MS-TMM and the optimization tech- nology based on genetic algorithms(GAs) are combined and extended. The identification problem is exchanged for an optimization problem, and it is formulated as a global minimum solution of the objective function with respect to natural frequencies of multibody system. At last, the numerical example of non-uniform beam with attach- ments is discussed, and the identification results indicate the feasibility and the effectivity of the proposed aop- proach.
基金support of National Natural Science Foundation of China(39970892)
文摘The ethanolic extracts of 100 common traditional Chinese drugs, which are widely used in many prescriptions in treatment of cancer in China, were screened for MDR of KBV200 cell line in vitro with MTT method. The result showed 9 extracts having MDR reversal activity. They were the extracts of Fructus Lagenariae Sicerariae, Radix Glycyrrhizae, Poria, Herba Andrographitis, Radix Sophorae Tonkinensis, Caulis Mahoniae, Folium Artemisiae Argyi, Rhizoma Curcumae, Fructus Cnidii. Other 5 extracts showed cytotoxic on KBV200 cell line. $$$$
文摘A proof is given that any λ _polynome over real quaternionic sfield can be factorized into produce of some linear factors.By the way,some properties and applications of this factorization in matrix theory are given.
文摘Based on the constructivism, using the College English(new edition) CD-ROMs for classroom teaching as main media, this paper explores the feasibility of the classroom teaching mode of appropriate combination of traditional methods with the multimedia overhead projector. The new teaching mode proves to be successful in that it has greatly improved the students' ability to use English in an all-round way and has obvious advantages over the only traditional method.
基金Supported by the National Natural Science Foundation of China(10476011)~~
文摘Numerical simulations are performed on the interface with large deformation induced by the interaction between a moving shock and two consecutive bubbles. The high performance of the level set method for multi-material interfaces is demonstrated. Discontinuous Galerkin finite element method is used to solve Euleri- an equations. And the fifth-order weighted essentially non-oscillatory (WENO) scheme is used to solve the level set equation for capturing multi-material interfaces. The ghost fluid method is used to deal with the interfacial boundary condition. Results are obtained for two bubble interacting with a moving shock. The contours of the constant density and the pressure at different time are given. In the computational domain, three different cases are considered, i.e. two helium bubbles, a helium bubble followed by an R22 bubble in the direction of the moving shock, and an R22 bubble followed by a helium bubble. Computational results indicate that multi-mate- rial interfaces can be properly captured by the level set method. Therefore, for problems involving the flow of three different materials with two different interfaces, each interface separating two different materials can be similarly handled.
基金Projects(11772188,11132007,11202126)supported by the National Natural Science Foundation of ChinaProject(11ZR1417000)supported by the Natural Science Foundation of Shanghai,China
文摘A forward recursive formulation based on corotational frame is proposed for flexible planar beams with large displacement.The traditional recursive formulation has been successfully used for flexible mutibody dynamics to improve the computational efficiency based on floating frame,in which the assumption of small strain and deflection is adopted.The proposed recursive formulation could be used for large displacement problems based on the corotational frame.It means that the recursive scheme is used not only for adjacent bodies but also for adjacent beam elements.The nodal relative rotation coordinates of the planar beam are used to obtain equations with minimal generalized coordinates in present formulation.The proposed formulation is different from absolute nodal coordinate formulation and the geometrically exact beam formulation in which the absolute coordinates are used.The recursive scheme and minimal set of dynamic equations lead to a high computational efficiency in numerical integration.Numerical examples are carried out to demonstrate the accuracy and validity of this formulation.For all of the examples,the results of the present formulation are in good agreement with results obtained using commercial software and the published results.Moreover,it is shown that the present formulation is more efficient than the formulation in ANSYS based on GEBF.
基金Supported by the National Natural Science Foundation of China (No.20776121) and the Specialized Research Fund for the Doctoral Program of Higher Education (No.20050530001), and the Scientific Research Fund of Hunan Provincial Education Department (No.07C765).
文摘The discrete particle method was used to simulate the distribution of gas holdup in a gas-liquid standard Rushton stirred tank. The gas phase was treated as a large number of bubbles and their trajectories were tracked with the results of motion equations. The two-way approach was performed to couple the interphase momentum exchange. The turbulent dispersion of bubbles with a size distribution was modeled using a stochastic tracking model, and the added mass force was involved to account for the effect of bubble acceleration on the surrounding fluid. The predicted gas holdup distribution showed that this method could give reasonable prediction comparable to the reported experimental data when the effect of turbulence was took into account in modification for drag coefficient.
文摘A decentralized task planning algorithm is proposed for heterogeneous unmanned aerial vehicle(UAV)swarm with different capabilities.The algorithm extends the consensus-based bundle algorithm(CBBA)to account for a more realistic and complex environment.The extension of the algorithm includes handling multi-agent task that requires multiple UAVs collaboratively completed in coordination,and consideration of avoiding obstacles in task scenarios.We propose a new consensus algorithm to solve the multi-agent task allocation problem and use the Dubins algorithm to design feasible paths for UAVs to avoid obstacles and consider motion constraints.Experimental results show that the CBBA extension algorithm can converge to a conflict-free and feasible solution for multi-agent task planning problems.
文摘Mutual information is widely used in medical image registration, because it does not require preprocessing the image. However, the local maximum problem in the registration is insurmountable. We combine mutual information and gradient information to solve this problem and apply it to the non-rigid deformation image registration. To improve the accuracy, we provide some implemental issues, for example, the Powell searching algorithm, gray interpolation and consideration of outlier points. The experimental results show the accuracy of the method and the feasibility in non-rigid medical image registration.
基金Project of Youth Fund from Education Department of Sichuan Province (No.07ZB050)
文摘GIS not includes only spatial data and attribute data, but also multimedia information. How to integrate and manage these data effectively is very important. With the development of the technology of Object-Relation DBMS (ORDBMS), it improved the capability of managing complicated information. With ORDBMS, GIS and multimedia information can be integrated together and can speed accessing files by using "buffer".
基金The Natural Science Foundation of China(No.11972193)the Science Challenge Project(No.TZ2016006-0104)。
文摘This study establishes the launch dynamics method,sensitivity analysis method,and multiobjective dynamic optimization method for the dynamic simulation analysis of the multiple launch rocket system(MLRS)based on the Riccati transfer matrix method for multibody systems(RMSTMM),direct differentiation method(DDM),and genetic algorithm(GA),respectively.Results show that simulation results of the dynamic response agree well with test results.The sensitivity analysis method is highly programming,the matrix order is low,and the calculation time is much shorter than that of the Lagrange method.With the increase of system complexity,the advantage of a high computing speed becomes more evident.Structural parameters that have the greatest influence on the dynamic response include the connection stiffness between the pitching body and the rotating body,the connection stiffness between the rotating body and the vehicle body,and the connection stiffnesses among 14^(#),16^(#),and 17^(#)wheels and the ground,which are the optimization design variables.After optimization,angular velocity variances of the pitching body in the revolving and pitching directions are reduced by 97.84%and 95.22%,respectively.
基金Project(61074074)supported by the National Natural Science Foundation,ChinaProject(KT2012C01J0401)supported by the Group Innovation Fund,China
文摘An improved model predictive control algorithm is proposed for Hammerstein-Wiener nonlinear systems.The proposed synthesis algorithm contains two parts:offline design the polytopic invariant sets,and online solve the min-max optimization problem.The polytopic invariant set is adopted to replace the traditional ellipsoid invariant set.And the parameter-correlation nonlinear control law is designed to replace the traditional linear control law.Consequently,the terminal region is enlarged and the control effect is improved.Simulation and experiment are used to verify the validity of the wind tunnel flow field control algorithm.
文摘Unquestionably, anyone born to Urhobo parents, being biologically Urhobo, should speak the Urhobo language. But does everybody born by an Urhobo parent speak Urhobo? From our observation, the answer to this question is clearly "no". Many of the Urhobo people in the Diaspora as well as those at home have chosen to shift from their own language to other languages. Hence Urhobo language has gradually acquired the status of a second language. Therefore, its teaching would also veer away from language teaching methods that are used for first languages. Based on Noam Chomsky's theory of Generative Grammar, aspects of the Urhobo grammar will be treated as we present specifically the verb "edia"/"to be". As it is, it is being presented as a second language bearing in mind the great population of Urhobo people in the Diaspora as well as Mowarin's "miguo generation" of Urhobo children back home in Urhobo land. This being so, the methods applied in this study are the Grammar Translation Method Approach as well as the Direct Method Approach. To facilitate its learning especially for the new generation of Urhobo persons, the poetic approach would also be applied.
文摘In this paper the large deflection dynamic problems of Euler beams are investigated. The vibration control equations are derived based on the multibody system method. A numerical procedure for solving the resulting differential algebraic equations is presented on the basis of the Newmark direct integration method combined with the Newton-Raphson iterative method. The sub beams are treated as small deformation in the convected coordinate systems, which can greatly simplify the deformation description. The rigid motions of the sub beams are taken into account through the motions of the convected coordinate systems. Numerical ex- amples are carried out, where results show the effectiveness of the proposed method.
基金Financial supported by the Gansu ProvincialSci. & Tech. Department (1002NKDA037)
文摘A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized with sucking-mouse adapted FMD virus (A/AV88(L) strain) were conjugated to colloidal gold beads and used as the capture antibody,and affinity purified polyclonal antibodies from rabbits which were immunized with cell-culture adapted FMD virus (A/CHA/09 strain) were used as detector antibody. On the nitrocellulose membrane of the immunochromatographic strip,the capture antibody was laid on a sample pad,the detector antibody was printed at the test line(T) and goat anti-guinea pigs IgG antibodies were immobilized to the control line(C). The lower detection limit of the test for a FMDV 146S antigen is 11.7ng/ml as determined in serial tests after the strip device was assembled and the assay condition optimization. No cross reactions were found with FMDV serotype C,Swine vesicular disease (SVD),Vesicular stomatiti svirus (VSV) and vesicular exanthema of swine virus (VES) viral antigens with this rapid test. Clinically,the diagnostic sensitivity of this test for FMDV serotypes A was 88.7% which is as same as an indirect-sandwich ELISA. The specificity of this strip test was 98.2% and is comparable to the 98.7% obtained with indirect-sandwich ELISA. This rapid strip test is simple,easy and fast for clinical testing on field sites; no special instruments and skills are required,and the result can be obtained within 15 min. To our knowledge,this is the first rapid immunochromatogarpic assay for serotype A of FMDV.
基金Projects(61105067,61174164)supported by the National Natural Science Foundation of ChinaProjects(012BAF10B11,2012BAF10B06)supported by the National Key Technologies R&D Program of China+1 种基金Project(F11-264-1-08)supported by the Shenyang Science and Technology Project,ChinaProject(2011BY100383)supported by the Cooperation Project of Foshan and Chinese Academy of Sciences
文摘Due to the effectiveness, simple deployment and low cost, radio frequency identification (RFID) systems are used in a variety of applications to uniquely identify physical objects. The operation of RFID systems often involves a situation in which multiple readers physically located near one another may interfere with one another's operation. Such reader collision must be minimized to avoid the faulty or miss reads. Specifically, scheduling the colliding RFID readers to reduce the total system transaction time or response time is the challenging problem for large-scale RFID network deployment. Therefore, the aim of this work is to use a successful multi-swarm cooperative optimizer called pseo to minimize both the reader-to-reader interference and total system transaction time in RFID reader networks. The main idea of pS20 is to extend the single population PSO to the interacting multi-swarm model by constructing hierarchical interaction topology and enhanced dynamical update equations. As the RFID network scheduling model formulated in this work is a discrete problem, a binary version of PS20 algorithm is proposed. With seven discrete benchmark functions, PS20 is proved to have significantly better performance than the original PSO and a binary genetic algorithm, pS20 is then used for solving the real-world RFID network scheduling problem. Numerical results for four test cases with different scales, ranging from 30 to 200 readers, demonstrate the performance of the proposed methodology.
文摘Robust video streaming through high error prone wireless channel has attracted much attention. In this paper the authors introduce an effective algorithm by joining the Unequal Error Protection ability of the channel multiplexing protocol H.223 Annex D, and the new H.263++ Annex V Data Partition together. Based on the optimal trade off of these two technologies, the Joint Source and Channel Coding algorithm can result in stronger error resilience. The simulation results have shown its superiority against separate coding mode and some Unequal Error Protection mode under recommended wireless channel error patterns.
文摘An ancient genome duplication (PPP1) that predates divergence of the cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot divergence in the genome of rice (Oryza sativa L.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. Our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice.