Integrating a DNA barcoding project with an ecological survey:a case study on temperate intertidal polychaete communities in Qingdao,China

In this study,we integrated a DNA barcoding project with an ecological survey on intertidal polychaete communities and investigated the utility of CO1 gene sequence as a DNA barcode for the classification of the intertidal polychaetes.Using 16S rDNA as a complementary marker and combining morphological and ecological characterization,some of dominant and common polychaete species from Chinese coasts were assessed for their taxonomic status.We obtained 22 haplotype gene sequences of 13 taxa,including 10 CO1 sequences and 12 16S rDNA sequences.Based on intra-and inter-specific distances,we built phylogenetic trees using the neighbor-joining method.Our study suggested that the mitochondrial CO1 gene was a valid DNA barcoding marker for species identification in polychaetes,but other genes,such as 16S rDNA,could be used as a complementary genetic marker.For more accurate species identification and effective testing of species hypothesis,DNA barcoding should be incorporated with morphological,ecological,biogeographical,and phylogenetic information.The application of DNA barcoding and molecular identification in the ecological survey on the intertidal polychaete communities demonstrated the feasibility of integrating DNA taxonomy and ecology.

未遂的浪漫私奔

我出生在一个军人家庭,初恋发生在我部队生活的后期,但被我父亲“扼杀”了。后来,我转业到地方,在一家康复医院工作。经人介绍,我认识了我现在的丈夫。 我丈夫当时的一切都让我及我的家庭挑不出毛病来,他的个头、长相、工作、为人处事、家庭背景。