Z971415 农一清在麦子上应用试验初报/杨正华,陈建辉(江苏省大丰市方强乡农科站)∥大麦科学.-1997,(1).-封四农一清是新推广的一种节约型、高肥效、多功能液体叶面喷施肥料,以每次每亩用农一清0.4 kg 对水60kg 喷施 KA-4B 大麦,调查亩...Z971415 农一清在麦子上应用试验初报/杨正华,陈建辉(江苏省大丰市方强乡农科站)∥大麦科学.-1997,(1).-封四农一清是新推广的一种节约型、高肥效、多功能液体叶面喷施肥料,以每次每亩用农一清0.4 kg 对水60kg 喷施 KA-4B 大麦,调查亩穗数、穗粒数、千粒重、迟熟穗(受冻后迟后蘖或穗)等性状,喷清水对照不如喷农一清的好,喷1次不如喷2次好。拔节后喷施可使亩增产20%～40%,喷施时间在3月下旬到4月上旬为宜,2次间隔7～10 d 为好。展开更多
A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel c...A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel chromatography. The molecular weight and isoelectric point (pI) of VP-Peco60-7 were determined to be approxi-mately 40 kDa and 4.1, respectively. By N-terminal sequence determination and peptide mapping analysis, VP-Peco60-7 was found to be similar to the versatile peroxidase isoenzyme VPL1, which was previously isolated from liquid cultures of the same species. However, the molecular weight and pI of VP-Peco60-7 were different from those of versatile peroxidases of liquid cultures, implying that the VP-Peco60-7 in this study is of a novel type. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a substrate, the maximal enzyme activity was obtained at 50 °C and pH 3.0. The catalysis of ABTS by VP-Peco60-7 was expressed by the Michaelis-Menten equa-tion. At 50 °C and pH 3.0, the maximum velocity (V max ) was 188.68 U·mg-1 and the michaelis constant (K m ) was 203.09 μmol·L-1 .展开更多
文摘Z971415 农一清在麦子上应用试验初报/杨正华,陈建辉(江苏省大丰市方强乡农科站)∥大麦科学.-1997,(1).-封四农一清是新推广的一种节约型、高肥效、多功能液体叶面喷施肥料,以每次每亩用农一清0.4 kg 对水60kg 喷施 KA-4B 大麦,调查亩穗数、穗粒数、千粒重、迟熟穗(受冻后迟后蘖或穗)等性状,喷清水对照不如喷农一清的好,喷1次不如喷2次好。拔节后喷施可使亩增产20%～40%,喷施时间在3月下旬到4月上旬为宜,2次间隔7～10 d 为好。
基金Supported by the Special Funds for Major State Basic Research Program of China （2007CB707805） the Natural Science Foundation of Zhejiang Province （Y505334）
文摘A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel chromatography. The molecular weight and isoelectric point (pI) of VP-Peco60-7 were determined to be approxi-mately 40 kDa and 4.1, respectively. By N-terminal sequence determination and peptide mapping analysis, VP-Peco60-7 was found to be similar to the versatile peroxidase isoenzyme VPL1, which was previously isolated from liquid cultures of the same species. However, the molecular weight and pI of VP-Peco60-7 were different from those of versatile peroxidases of liquid cultures, implying that the VP-Peco60-7 in this study is of a novel type. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a substrate, the maximal enzyme activity was obtained at 50 °C and pH 3.0. The catalysis of ABTS by VP-Peco60-7 was expressed by the Michaelis-Menten equa-tion. At 50 °C and pH 3.0, the maximum velocity (V max ) was 188.68 U·mg-1 and the michaelis constant (K m ) was 203.09 μmol·L-1 .
