目的:设计并构建偶联双基因靶点的多功能金纳米棒颗粒(TKI-258-p GNRs@m Si O2-RGD),并探讨该多功能金纳米棒颗粒对人三阴性乳腺癌MDA-MB-231细胞的细胞毒性,初步明确该新型多功能金纳米棒颗粒在体外实验中所需的剂量范围。方法:运用种...目的:设计并构建偶联双基因靶点的多功能金纳米棒颗粒(TKI-258-p GNRs@m Si O2-RGD),并探讨该多功能金纳米棒颗粒对人三阴性乳腺癌MDA-MB-231细胞的细胞毒性,初步明确该新型多功能金纳米棒颗粒在体外实验中所需的剂量范围。方法:运用种子介导生长法制备金纳米棒,以改良的奥伯法对其表面进行介孔二氧化硅包裹,通过表面修饰偶联整合素αvβ3和表皮生长因子受体(VEGFR)的特异性抑制剂RGD和TKI-258,采用紫外分光光度计和透射电镜对其特征进行检测。以不同浓度(0、12.5、25、50、100、200μg/ml)多功能金纳米棒颗粒孵育人三阴性乳腺癌MDA-MB-231细胞株,采用CCK-8法检测细胞增殖率,并进行细胞毒性评级。结果:多功能金纳米棒颗粒物理状态稳定,吸收峰位于515、860 nm处。偶联的双基因靶点抑制剂TKI-258、RGD分别在360、204 nm处有特征性吸收峰,药物包封率分别为58.18%±7.13%,57.95%±5.22%,载药率分别为4.26%±0.42%,4.35%±0.79%。不同浓度多功能金纳米棒处理后,细胞增殖率在50%-100%之间,细胞毒性评级为I-II级,0-100μg/ml浓度范围内无细胞毒性。结论:偶联双基因靶点的多功能金纳米棒颗粒(TKI-258-p GNRs@m Si O2-RGD)具有热疗/放疗增敏的特殊光学性质,偶联双基因靶点抑制剂TKI-258、RGD的载药量稳定,生物相容性良好,可作为三阴性乳腺癌细胞特异性靶向的热疗/放疗载药增敏材料。展开更多
Recent developments in nanochemistry offer precise morphology control of nanomaterials, which has significant impacts in the field of heterogeneous catalysis. Rational design of bifunctional catalysts can influence va...Recent developments in nanochemistry offer precise morphology control of nanomaterials, which has significant impacts in the field of heterogeneous catalysis. Rational design of bifunctional catalysts can influence various aspects of catalytic properties. In this review, a new class of bifunctional catalysts with a metal@silica yolk-shell nanostructure is introduced. This structure has many advantages as a heterogeneous catalyst since it ensures a homogeneous environment around each metal core, and particle sintering is effectively eliminated during high temperature reactions. The catalysts exhibit high activity and recyclability in gas- and solution-phase reactions. It is anticipated that appropriate selection of bifunctional components and optimal structural control will significantly further enhance the catalytic properties, and enable target reaction-oriented development of new catalysts.展开更多
文摘目的:设计并构建偶联双基因靶点的多功能金纳米棒颗粒(TKI-258-p GNRs@m Si O2-RGD),并探讨该多功能金纳米棒颗粒对人三阴性乳腺癌MDA-MB-231细胞的细胞毒性,初步明确该新型多功能金纳米棒颗粒在体外实验中所需的剂量范围。方法:运用种子介导生长法制备金纳米棒,以改良的奥伯法对其表面进行介孔二氧化硅包裹,通过表面修饰偶联整合素αvβ3和表皮生长因子受体(VEGFR)的特异性抑制剂RGD和TKI-258,采用紫外分光光度计和透射电镜对其特征进行检测。以不同浓度(0、12.5、25、50、100、200μg/ml)多功能金纳米棒颗粒孵育人三阴性乳腺癌MDA-MB-231细胞株,采用CCK-8法检测细胞增殖率,并进行细胞毒性评级。结果:多功能金纳米棒颗粒物理状态稳定,吸收峰位于515、860 nm处。偶联的双基因靶点抑制剂TKI-258、RGD分别在360、204 nm处有特征性吸收峰,药物包封率分别为58.18%±7.13%,57.95%±5.22%,载药率分别为4.26%±0.42%,4.35%±0.79%。不同浓度多功能金纳米棒处理后,细胞增殖率在50%-100%之间,细胞毒性评级为I-II级,0-100μg/ml浓度范围内无细胞毒性。结论:偶联双基因靶点的多功能金纳米棒颗粒(TKI-258-p GNRs@m Si O2-RGD)具有热疗/放疗增敏的特殊光学性质,偶联双基因靶点抑制剂TKI-258、RGD的载药量稳定,生物相容性良好,可作为三阴性乳腺癌细胞特异性靶向的热疗/放疗载药增敏材料。
文摘Recent developments in nanochemistry offer precise morphology control of nanomaterials, which has significant impacts in the field of heterogeneous catalysis. Rational design of bifunctional catalysts can influence various aspects of catalytic properties. In this review, a new class of bifunctional catalysts with a metal@silica yolk-shell nanostructure is introduced. This structure has many advantages as a heterogeneous catalyst since it ensures a homogeneous environment around each metal core, and particle sintering is effectively eliminated during high temperature reactions. The catalysts exhibit high activity and recyclability in gas- and solution-phase reactions. It is anticipated that appropriate selection of bifunctional components and optimal structural control will significantly further enhance the catalytic properties, and enable target reaction-oriented development of new catalysts.
