基于多重响应的钢渣超高性能混凝土组成优化设计

为了优化生态超高性能混凝土(UHPC)的性能,提高钢渣的利用率,文章从材料和实验方法、二维优化设计模型的建立、钢渣生态超高性能混凝土成分优化设计出发,探讨了基于多重响应的钢渣超高性能混凝土组成优化设计。

Geochip-based analysis of microbial functional genes diversity in rutile bio-desilication reactor

Biological desilication process is an effective way to remove silicate from rutile so that high purity rutile could be obtained. However, little is known about the molecular mechanism of this process. In this work, a newly developed rutile bio-desilication reactor was applied to enrich rutile from rough rutile concentrate obtained from Nanzhao rutile mine and a comprehensive high through-put functional gene array(Geo Chip 4.0) was used to analyze the functional gene diversity, structure and metabolic potential of microbial communities in the biological desilication reactor. The results show that TiO2 grade of the rutile concentrate could increase from 78.21% to above 90% and the recovery rate could reach to 96% or more in 8-12 d. The results also show that almost all the key functional genes involved in the geochemical cycling process, totally 4324 and 4983 functional microorganism genes, are detected in the liquid and ore surface, respectively. There are totally 712 and 831 functional genes involved in nitrogen cycling for liquid and ore surface samples, respectively. The relative abundance of functional genes involved in the phosphorus and sulfur cycling is higher in the ore surface than liquid. These results indicate that nitrogen, phosphorus and sulfur cycling are also present in the desiliconization process of rutile. Acetogenesis genes are detected in the liquid and ore surface, which indicates that the desiliconizing process mainly depends on the function of acetic acid and other organic acids. Four silicon transporting genes are also detected in the sample, which proves that the bacteria have the potential to transfer silicon in the molecule level. It is shown that bio-desilication is an effective and environmental-friendly way for enrichment of rough rutile concentrate and presents an overview of functional diversity and structure of desilication microbial communities, which also provides insights into our understanding of metabolic potential in biological desilication reactor ecosystems.

Synergistic promotion of HER and OER by alloying ternary Zn‐Co‐Ni nanoparticles in N‐doped carbon interfacial structures

Catalytic water splitting potentially reduce the consumption of fossil fuels and has received intense research attention.Synergy effects in multi‐element transition metal‐based water splitting catalysts have evoked special interests.Studies on catalysts in interfacial structures are especially meaningful due to their pertinence in applications.In this study,we report the synergy effects in promoting catalytic power in the ternary transition metal Zn,Co,Ni alloy nanoparticles that embeds in the carbonized Ppy/CNT multilayered matrix.By comparison with a series of binary or single metal counterparts,the mechanism under the synergy effects are elucidated.Experimental and DFT calculation results indicate that the ternary transition metal catalysts in the N‐doped carbon matrix present special electronic structure,which benefits the reversible transition‐state adsorption in HER and OER and render the catalysts high conductivity in room temperature.We expect our findings inspire further development of efficient transition metal HER and OER catalysts.

Analysis of Absorption and Reaction Kinetics in the Oxidation of Organics in Effluents Using a Porous Electrode Ozonator

A novel electrode design for the in situ generation of ozone in the reaction zone of a tubular reactor is described in this work.The ozone generator uses a porous inner electrode tube in the corona discharge assembly,and the ozone generated around the outer periphery of the porous tube diffuses into the tubular reactor and reacts with the contaminants in the fluid that is being treated.A mathematical model that includes absorption and second order reaction in the film is developed to describe ozonation kinetics of a contaminant dye in the tubular reactor.The model describes the experimental data for dye decolorization,oxidation byproducts,dissolved ozone,and ozone gas concentrations well.Model analysis indicates that the fast dye decolorization reaction occurs partly in the liquid film and partly in the bulk fluid.The model can be used in the selection of appropriate gas-liquid contactors for efficient oxidation of contaminants in effluents.

Behavioural Response of Anacanthotermes ochraceus towards Some Baits and Volatile Oils

Six types of termites＇ baits （cartoon, rough tissues, soft toilet tissues, acacia wood, saw dust of building wood and sackcloth） were elicited variable degree of attraction toward Anacanthotermes ochaceus, where the best one was cardboard, followed by soft and rough toilet tissue at mono-choice and multiple-choice tests. Chemical analysis showed that there were variations in the chemical constituents （lignin, cellulose, N, H and C） of the six baits studied. On the other hand, olfactory reactions of harvester termites toward eight volatile oils （four natural medical plants and four commercial terpines） were repellent, but the most repellent one was cineol and the least one was clove oil. The most tested oils were caused inhibition in feeding, except cedar wood. The most toxic oils were cineol followed by thymol.

Mutational analysis of the PTEN gene in soft tissue sarcomas

Objective： This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas （STS）. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to amplify 4 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in soft tissue sarcomas. Results： Two of 86 cases showed 130th condon G→A missense mutation in the exon 8 of PTEN gene, and this mutation made Arg to change to Gin in PTEN protein structure 334th condon A→T missense mutation in the exon 8 of PTEN gene, and this mutation made Asn to change to Lys in PI-EN protein structure. Conclusion： These data indicated the existence of PTEN mutation in soft tissue sarcomas, but PTEN gene mutation rate is very low. PTEN mutation may prays an less important role in the development and malignant transformation of soft tissue sarcomas.

Carrier detection and prenatal diagnosis of hemophilia A

Objective To establish an effective laboratory examination system for carrier detection and prenatal diagnosis of haemophilia A (HA) with a variety of molecular biological methods which are simple, rapid and easy to use. Methods Detection of inversion involving intron 22 in the FVIII gene was completed by long distance polymerase chain reaction ( PCR) and linkage analysis was performed by using several genetic polymorphisms including an intragenic Bcl I RFLP, 2 STRs and an extragenic St14 VNTR. Results Intron 22 inversion was observed in 10 out of the 21 (47.6%) pedigrees examined. Prenatal diagnosis was completed in 3 pedigrees. A further combination of the four intragenic and extragenic polymophic loci gave an informative rate of 94.7%. Conclusions Female relatives in HA families with inversion can be detected with direct diagnostic procedure. The application of long distance PCR makes the detection much more simple and rapid. For families without inversions, it is easier and more cost-effective to undertake linkage analysis of genetic polymorphism based on PCR.

Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China

OBJECTIVE: To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region. METHODS: The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed. RESULTS: Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands. CONCLUSION: Nan Peng Lie Islands is tsutsugamushi disease nature foci.

A multiple-reaction-monitoring mass spectrometric method for simultaneous quantitative analysis of five plasma apolipoproteins

A multiplexed targeted proteomic assay using a mTRAQ-MRM/MS-based approach was developed and assessed to systematically quantify the relative expressions of five candidate plasma apolipoproteins that have been previously shown to be dysregulated in neuropsychiatric disorders and cognitive dysfunction:apolipoprotein H(APOH),apolipoprotein J(APOJ),apolipoprotein A4(APOA4),apolipoprotein E(APOE),and apolipoprotein D(APOD).The peptides and transitions of each APO were carefully selected according to the tandem MS signals acquired on a TripleTOFTM 5600,followed by optimization of the declustering potential and collision energy voltages for transitions on a QTRAP 5500.Our results showed that the collision energies of mTRAQ-labeled peptides were approximately 15%–20%higher than corresponding non-labeled peptides.Through optimized transitions and parameters,we analyzed the relative abundances of the five APOs in human plasma with and without depletion of high abundant proteins.The results indicated that the MRM signals of four target APOs were significantly increased after depletion,while the MRM signal of one APO,APOD,was decreased.Furthermore,the relative abundances of the five target APOs in healthy human plasma were stable,and the ranking of these proteins according to their MS responses changed slightly.Therefore,we deduced that the rank order of the MS signals for these target proteins can be developed as a diagnostic signature for diseased plasma.