应用多元PCR技术对我国红火蚁社会型的鉴定

红火蚁Solenopsis invicta Buren蚁群存在着单后型和多后型。不同型的红火蚁在行为及生态上存在着较大的差异,因此社会型的鉴别是一个地区制定正确的红火蚁监控技术的关键步骤。本文利用多元PCR技术首次对采自我国4省16个县(市、区)的120个红火蚁蚁群的社会型进行了鉴别。结果表明:我国的红火蚁多后型与单后型共存,但以多后型为主。多后型与单后型的比约为4∶1(97∶23),但会因地区的不同而异:如广东多后型与单后型的比例最低,约为3∶1(66∶20),而广西的多后型与单后型的比为7∶1(21∶3)。最后,根据我国红火蚁的发生危害情况对这些结果进行了讨论。

红火蚁的社会型及其相关基因Gp-9的研究综述

红火蚁Solenopsis invicta Buren的蚁巢具有2种基本的社会组织形态:单后型和多后型。蚁巢中蚁后的数量是由工蚁和蚁后的基因型共同决定的。红火蚁的Gp-9基因编码一种气味结合蛋白并控制这种社会多态性的表达。Gp-9基因的发现首次证明单个基因在昆虫复杂的社会行为中起关键的作用。从红火蚁的社会型特征、Gp-9基因与不同社会型的关系以及Gp-9基因的相关研究对该领域的研究进展进行综述。

深圳红火蚁蚁巢密度、社会型研究及防治效果评价

本文报道了深圳红火蚁生境类型与蚁巢密度的关系，社会型的鉴别以及应用茚虫威对多后型和单后型、多后型共存地区的防治效果。红火蚁的蚁巢密度（巢／100m2）与生境类型密切相关，在所调查的5类生境中，待建地的蚁巢密度最高，为3．81巢／100m2，公路边的次之，为2．95巢／100群，城市绿地的最低，为0．63巢／100m2。应用多元PCR技术对6个行政区社会型进行鉴别的结果表明，福田、龙岗、南山区为多后型与单后型共存，单后型与多后型的比仅为1：（3～4）。罗湖、宝安、盐田3区全部为多后型；多后型地区的蚁巢密度（2．2巢／100m2）比单后型地区（L9巢／100m2）的为高，但没有显著差异；应用茚虫威进行防治60d后的调查结果表明，多后型地区的防治效果（97．8％）比单后型地区（96．7%）的要好，但没有显著差异。最后，本文对这些结果产生的原因及应用价值进行了讨论。

红火蚁雌蚁生殖发育的研究

通过解剖多后型红火蚁Solenopsis invicta Buren雌蛹、有翅雌蚁和脱翅雌蚁,观察了雌蚁内生殖系统的结构和发育进度。结果表明,多后型雌蚁内生殖系统形成于蛹期,其结构与单后型雌蚁存在一定差异。从发育进度来看,虽然雌蚁羽化后其卵巢大小和卵巢管数量仍有一定增长,但蛹期是卵巢大小、卵巢管数量快速增长的阶段;卵子发生始见于羽化后第4d,且卵量随个体发育而快速增加,直至脱翅后第10d达到高峰,随后开始下降;生殖系统的其它结构的变化较小。雌蛹、有翅雌蚁和脱翅雌蚁发育后期的卵巢平均大小分别为1149.23×712.42、1594.88×834.26和1975.60×1303.57μm;而卵巢管数量则分别为62.67、81.33和86.33条。至雌蚁脱翅后10d,卵巢中成熟卵和总卵量分别为20.00±4.62粒和34.00±2.31粒。

Role of genetics in the diagnosis and prognosis of Crohn's disease

Considering the epidemiological, genetic and immunological data, we can conclude that the inflammatory bowel diseases are heterogeneous disorders of multifactorial etiology in which hereditability and environment interact to produce the disease. It is probable that patients have a genetic predisposition for the development of the disease coupled with disturbances in immunoregulation. Several genes have so far been related to the diagnosis of Crohn's disease. These genes are related to innate pattern recognition receptors, to epithelial barrier homeostasis and maintenance of epithelial barrier integrity, to autophagy and to lymphocyte differentiation. So far, the strongest and most replicated associations with Crohn's disease have been demonstrated with NOD2 , IL23R and ATG16L1 genes. Many genes have so far been implicated in the prognosis of Crohn's disease and many attempts have been made for classification of genetic profiles in Crohn's disease.CARD15 seems to be not only a susceptibility gene, but also a disease-modifier gene for Crohn's disease. Enriching our understanding of Crohn's disease genetics is of value, but when combining genetic data with functional data the outcome could be of major importance to clinicians.

Conditional Quantile Polynomial Distributed Lag Model with an Application to Rubber Price Returns

Impacts of returns on assets are not instantaneously felt, since there is lag period. In this paper we consider the problem of developing a model for the conditional QPDL （quantile polynomial distributed lag） and investigate the influences of the conditioning variables on the location, scale and shape parameters of the QPDL model. As an economic application, we consider the production of rubber and its price returns ofSri Lanka. From the analysis we observed that the QPDL model applications were better estimators than the PDL （Polynomial Distributed Lag） models.

A hybrid discrete particle swarm optimization-genetic algorithm for multi-task scheduling problem in service oriented manufacturing systems

To cope with the task scheduling problem under multi-task and transportation consideration in large-scale service oriented manufacturing systems(SOMS), a service allocation optimization mathematical model was established, and then a hybrid discrete particle swarm optimization-genetic algorithm(HDPSOGA) was proposed. In SOMS, each resource involved in the whole life cycle of a product, whether it is provided by a piece of software or a hardware device, is encapsulated into a service. So, the transportation during production of a task should be taken into account because the hard-services selected are possibly provided by various providers in different areas. In the service allocation optimization mathematical model, multi-task and transportation were considered simultaneously. In the proposed HDPSOGA algorithm, integer coding method was applied to establish the mapping between the particle location matrix and the service allocation scheme. The position updating process was performed according to the cognition part, the social part, and the previous velocity and position while introducing the crossover and mutation idea of genetic algorithm to fit the discrete space. Finally, related simulation experiments were carried out to compare with other two previous algorithms. The results indicate the effectiveness and efficiency of the proposed hybrid algorithm.

Emergence of recombinant PCV2 between genotype 2a and 2b in pig herds in Shandong province of China

Since late 2005, the swine industry in Shandong province has experienced a significant increase in postweaning multisystemic wasting syndrome （PMWS）. Porcine circovirus type 2 （PCV2） is one of the major etiologic agents of PMWS. In order to understand the genetic diversity of PCV2 in swine herds in Shandong province, we sequenced six complete genomes of PCV2 between 2005 and 2007. Sequence analysis revealed that all the six PCV2 strains belonged to one genotype （named PCV 2b）. The deduced amino acid sequences of the replication associated protein （coded by ORFI） showed that all the six PCV2 strains belonged to genotype 2a and could be further divided into two clusters. Based on the analysis of the capsid protein （coded by ORF2）, the six PCV2 strains belonged to genotype 2b. All the six strains belonged to recombinant viruses between genotype PCV2a and genotype PCV2b. This study implied that such recombinant PCV2 spread widely in Shandong area and might be the reason that pig death rate associated with PMWS increased in recent years.

Genetic Diversity of Intragenomic Rearrangement of Porcine Circovirus Type 2 in vitro and in vivo

We characterized the genome sequences of defective-interfering(DI) particle DNA of porcine circovirus type 2(PCV2) by sequencing and bioinformatics analyses. DI particles were both generated by serial passage of PCV2 in PK15 cells and obtained from sera of pigs with postweaning multisystemic wasting syndrome(PMWS). These subviral isolates ranged from 358 nt to 1 125 nt genomes. Investigating the complexity and diversity of PCV2 DI in vivo and in vitro can help elucidating the evolutionary history of PCV2.

Bayesian Variable Selection via Perfect Gibbs Coupler Using Approximate Bounds

Variable selection is one of the most fundamental problems in regression analysis. By sampling from the posterior distributions of candidate models, Bayesian variable selection via MCMC （Markov chain Monte-Carlo） is effective to overcome the computational burden of all-subset variable selection approaches. However, the convergence of the MCMC is often hard to determine and one is often not sure about if obtained samples are unbiased. This complication has limited the application of Bayesian variable selection in practice. Based on the idea of CFTP （coupling from the past）, perfect sampling schemes have been developed to obtain independent samples from the posterior distribution for a variety of problems. Here the authors propose an efficient and effective perfect sampling algorithm for Bayesian variable selection of linear regression models, which independently and identically sample from the posterior distribution of the model space and can efficiently handle thousands of variables. The effectiveness of the authors＇ algorithm is illustrated by three simulation studies, which have up to thousands of variables, the authors＇ method is further illustrated in SNPs （single nucleotide polymorphisms） association study among RA （rheumatoid arthritis） patients.

Jiāwèi Wǔbèizǐsǎn(Supplemented Galla Chinensis Powder) application on the navel for different syndromes of profuse sweating after tumor chemotherapy

Objective To observe the clinical efficacy of Jiāwèi Wǔbèizǐ sǎn（加味五倍子散,Supplemented Galla Chinensis Powder） application on the different syndromes of profuse sweating after tumor chemotherapy.Methods One hundred and sixty patients with profuse sweating after tumor chemotherapy were randomly divided into a control group（n=80） and a treatment group（n=80）.Placebo application was carried out in the control group,and traditional Chinese medicine application was conducted in the treatment group.Treatment for 7 days was considered as 1 course of treatment.Results In treatment group,48 patients were cured,improvement was found in25 cases,and ineffectiveness was seen in 7 cases.For the patients with lungwei insecurity syndrome,19 cases were cured,improvement was found in10 cases,and ineffectiveness was seen in 1 case;for the patients with deficiency of both qi and yin,27 cases were cured,improvement was found in 9 cases,and ineffectiveness was seen in 2 cases;for the patients with vigorous fire due to yin deficiency,3 cases were cured,improvement was found in5 cases,and ineffectiveness was seen in 4 cases.According to statistical analysis,the total efficacy of treatment group was superior to that of control group（P0.05）.The efficacy on lung-wei insecurity and deficiency of both qi and yin of treatment group was superior to that of control group（both P0.05）,but there was no obvious difference between treatment group and control group in the efficacy on vigorous fire due to yin deficiency（P0.05）.Conclusion Supplemented Galla Chinensis Powder application on the navel can effectively improve the profuse sweating syndrome of patients in the types of lung-wei insecurity and deficiency of both qi and yin after tumor chemotherapy,but the efficacy is not so good for the patients in the type of vigorous fire due to yin deficiency.