鸡毒霉形体HS株pMGA多基因族的研究

鸡毒霉形体 (Mycoplasma gallisepticum ,GM)基因组中存在着编码细胞表面血凝粘附素蛋白 (protein of My-coplasma gallisepticum adhesin,p MGA)相关基因组的多基因族。为筛选出含有完整的 p MGA基因片段 ,并估算出鸡毒霉形体 HS株基因组中 p MGA多基因族的基因数 ,本试验以 p UC1 8为载体 ,用 Eco R 限制性内切酶构建了鸡毒霉形体 HS株的基因组文库。根据 p MGA基因引导序列的保守区和 p MGA基因间隔区的序列 ,人工合成了 2个寡核苷酸探针 (探针 、探针 ) ,经标记后 ,双筛选所建的基因文库 ,从 6 0 0个转化子中共得到的 38个含有 p MGA基因的阳性克隆子 ,选其中 1个 7.5 kb的片段 (17号阳性克隆子 ,W17) ,用 4种限制性内切酶 (Eco R ,Hind ,Pst ,Bgl )酶切消化 ,绘制了该片段的物理图谱。该片段酶切产物经电泳、转膜后与探针 和探针 杂交 ,发现 2个探针杂交图谱基本相同 ,根据杂交带及放射信号的强度值 ,估测出该片段含有 4个 p MGA基因 ,以这个片段所含的 p MGA基因数为标准 ,估算出鸡毒霉形体 HS株含有 39个 p MGA基因。

基于多基因族编码的股票网络社团划分研究

针对传统股票网络社团划分算法发现精度低、时间复杂度高、容易陷入局部最优解的缺点,提出一种基于多基因族(MGF)编码的基因表达式编程(GEP)股票网络社团划分算法,来研究股票市场复杂网络社团化现象。该算法利用多基因族编码的特性,将代表股票节点的ID号和表示社团的类型分别编码在两个不同的多基因族中,再通过一个映射函数将两者的相互作用关系隐式编码在染色体中;同时,将精英迁移策略应用到基因选择、交叉、倒置、限制交换等各个遗传阶段,以避免早熟现象,加快遗传收敛到全局最优解的速度。实验分析表明,该算法能够准确和高效地实现股票复杂网络社团的划分,其划分结果对投资者进行决策具有重要的指导意义。

鸡毒霉形体HS株pMGA多基因族序列分析

用限制性核酸内切酶对鸡毒霉形体 (MG) HS株基因文库中经初步估测可能含有的 4个鸡毒霉形体粘附蛋白(protein of Mycoplasma gallisepticumadhesin,p MGA)基因的 Mg W1 7重组质粒进行了不同组合的酶消化 ,根据消化片段的大小及酶切位点绘制了 7.5 kb外源片段的物理图谱 ;然后根据所得的物理图谱 ,选用 Pst 和 Eco R 将Mg W1 7外源片段酶解成 1 .3、2 .0、4 .2 kb3个部分 ,将它们分别亚克隆到 SK( + ) 质粒上 ,得到了 3个亚克隆子SGp1 0 0、SGp2 0 0、SGp30 0。使用核酸外切酶 缺失法构建缺失子系列 ,经序列分析后得到 Mg W1 7外源片段的全部序列。序列全长 74 34bp,中间含有 2个完整的 p MGA基因和另 2个不完整的 p MGA基因的首部和尾部 ,分别将它们顺次命名为 H- p MGA1 .1、H- p MGA1 .2、H- p MGA1 .3和 H- p MGA1 .4。 2个完整的 p MGA基因的阅读框长度分别为1 96 7bp(1 .2 )和 2 0 39bp(1 .3) ;H- p MGA1 .1首部不完整的阅读框的长度为 72 0 bp,尾部不完整的 H- p MGA1 .4的长度为 1 75 2 bp。将 H- p MGA基因与已报道的 MG.S6株、F株的 p MGA基因进行了比较 ,探讨了 p MGA基因在不同MG菌株中的相似性、基因启动子结构的差异、间隔区内

miR-17-92基因簇研究进展

微小RNA(microRNA,miRNA)是一类内源性的非编码的高度保守的单链小分子RNA,通过完全和(或)部分互补的原则结合到靶基因3′UTRs或者直接剪切靶基因mRNA,以降解或抑制mRNA翻译的方式调控靶基因的表达。miR-17-92基因簇位于人染色体13q31.3处,可产生miR-17,miR-18,miR-19a,miR-20,miR-19b和miR-92等6个成熟的miRNA。miR-17-92基因簇通过调控30多种靶基因参与肿瘤的发生发展。综述miR-17-92基因簇的结构和功能,及其在妇科及其他肿瘤发生发展中的作用。

肝癌微环境中影响转移相关通路的基因富集分析

目的 探讨肝癌微环境对肝癌发生门静脉转移起重要作用的基因簇或通路,为肝癌的治疗和研究提供理论依据.方法 利用基因组富集（GSEA）的方法,对美国国家生物技术信息中心（NCBI）的综合性的基因表达与杂交排序数据库（GEO数据库）中的11例肝癌无门静脉癌栓（PVTT）和9例肝癌合并PVTT的癌旁相对正常组织基因表达谱芯片进行分析,分析其在肿瘤相关通路中的富集特征与核心富集基因.结果 找到15条相关的信号通路,这些通路与癌旁微环境中的免疫功能状态有关,对于肝癌发生PVTT转移可能起重要作用.结论 肝癌发生门静脉转移除了与肿瘤细胞本身的恶性增殖和侵袭有关以外,还与周围微环境中的免疫状态和炎症反应有重要联系.

Smith-Fineman-Myers综合征的候选基因GPC4分析

目的:探讨GPC4基因与中国山东Smith-Fineman-Myers综合征(SFMS)的关系,并分析SFMS患者GPC4基因突变。方法:利用primer3设计扩增GPC4全部编码序列及内含子和外显子接头序列的引物,采用PCR扩增结合PCR产物直接测序方法检测GPC4基因开放性阅读框架区域基因突变。结果:在GPC4基因开放性阅读框架区域内并未检测到导致疾病的基因突变。结论:山东SFMS家系患者不是由于GPC4基因编码区域基因突变所致。

中国广西人群miRNA-17-92基因簇启动子区rs1491034和rs982873遗传多态性研究

目的研究rs1491034和rs982873单核苷酸多态性(SNPs)在广西人群中的分布特点,比较二者基因型和等位基因在不同种族、地区人群间的分布差别。方法采用SNaPshot SNP分型技术和DNA测序法检测397例广西人群rs1491034和rs982873SNPs,比较两位点基因型和等位基因频率与国际人类基因组单体型图(HapMap)计划公布的不同种族和地区人群间的差别。结果广西人群rs1491034和rs982873均存在TT,CT及CC 3种基因型。2个位点的基因型和等位基因频率在广西人群不同性别间比较,差别均无统计学意义(P>0.05)。广西人群rs1491034基因型和等位基因频率与HapMap-CEU和HapMap-YRI人群比较,差别均有统计学意义(P<0.01),而rs982873基因型和等位基因频率与HapMap-JPT和HapMap-YRI人群比较,差别均有统计学意义(P<0.05),但两位点基因型和等位基因频率与其他人群比较,差别均无统计学意义(P>0.05)。结论广西人群存在rs1491034和rs982873多态性,与其他种族、地区人群比较存在不同程度的差别。

基于基因表达式编程的复杂网络研究

论文提出一种基于基因表达式编程的复杂网络研究,用两个多基因族分别表示节点和社团类型,且通过一个映射函数表达两者的相互作用,再进行基因选择、交叉等遗传阶段。实验分析表明,该算法解决社团划分问题具有更高的效率和精确度。

高等植物中的多酚氧化酶

介绍了多酚氧化酶在植物体内的分布、性质、分子结构、生理作用及分子生物学研究进展。

载脂蛋白AⅠ-CⅢ基因簇多态性在心脑血管疾病低发的云南德宏傣族中的分布情况

目的探讨载脂蛋白(apolipoprotein,apo)AⅠ-CⅢ基因簇的apoAⅠ/MspⅠ和apoCⅢ/SstⅠ基因多态性在心脑血管疾病低发的云南德宏傣族人群中的分布情况。方法2002年4～5月在云南德宏和昆明两地随机抽样168名傣族和72名汉族人,提取基因组DNA后,应用聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polym orphism,PCR-RFLP)技术检测apoAⅠ-CⅢ基因簇中apoAⅠ启动子区MspⅠ多态性(-75bpG/A和+83bpC/T)和apoCⅢ3′端非编码区的SstⅠ多态性。结果apoAⅠ-CⅢ基因簇的apoAⅠ/MspⅠ和apoCⅢ/SstⅠ的等位基因M1+、M1-、M2+、M2-、S+、S-在傣汉两民族的分布频率依次为傣族:0.735、0.265、0.938、0.062、0.310、0.690;汉族:0.715、0.285、0.917、0.083、0.347、0.653。apoAⅠ-CⅢ基因簇的这6个等位基因频率在傣汉两民族间无统计学意义(P>0.05)。比较分析21个不同人群apoAⅠ/MspⅠ基因多态性和35个不同人群apoCⅢ/SstⅠ基因多态性的结果表明,德宏傣族人群apoAⅠ-CⅢ基因簇多态性分布与同属于蒙古人种的人群接近,而与高加索人种和尼格罗人种有较大的差异性。结论apoAⅠ-CⅢ基因簇的apoAⅠ基因启动子区-75bp和+83bp位点的基因多态性可能是德宏傣族人群心脑血管疾病患病率较低的重要遗传因素。而apoCⅢ的S+等位基因在该人群中并不是心脑血管疾病患病的易感因子。傣族人群具有较低的心脑血管疾病患病率应归因于遗传因素和环境因素协同作用的结果。

RFLP技术及其在大麦研究中的应用

本文扼要地阐述了限制性片段长度多态性(RFLP)技术的原理和方法,着重介绍了该项技术在大麦研究上的应用和进展,其中包括:(1)大麦α—淀粉酶多基因族的RFLP分析;(2)RFLP标志在大麦抗病育种中的应用;(3)RFLP技术与大麦籽粒醇溶蛋白的研究;(4)构建大麦RFLP连锁图。

微小RNA-106b参与内皮细胞介导的血管新生作用机制研究

目的研究微小RNA(microRNA,miR)-106b是否参与动脉粥样硬化相关的血管新生,明确miR-106b在内皮细胞中参与血管新生的作用机制。方法内皮细胞培养并转染miR-106b,分为miR-106b组、空白对照组和阴性对照组。提取RNA、反转录及实时定量PCR检测miR-106b相对表达量以明确转染效率。观察各组内皮细胞在凝固的基质胶中管腔形成情况。TUNEL法检测转染后细胞凋亡状况并进行靶基因的预测,采用Western blot法检测筛选的蛋白相对表达量变化。结果与空白对照组和阴性对照比较,miR-106b组在基质胶中管腔形成明显减少;与阴性对照组比较,miR-106b组管腔比值、信号转导及转录激活因子3mRNA相对表达量及蛋白表达明显降低(P<0.05,P<0.01)。阴性对照组与miR-106b组细胞凋亡率比较,差异无统计学意义(1.19%vs 3.39%,P>0.05)。结论 miR-106b在内皮细胞抑制血管新生可能是通过下调信号转导及转录激活因子3,与血管内皮生长因子无直接关系。

儿童神经病

9926542 癫痫患儿过早停用抗癫痫药物的随机前瞻性研究/Peters A C B//Neurol-ogy.-1998,50(3).-724～730 湘医图9926543 有高热惊厥病史患儿的癫痫的诊断及定位/Hamati-Haddad A//Neurol-ogy.-1998,50(4).-917～922 湘医图9926544 IA 型17P 多基因族夏科马里病婴幼儿:临床和电生理研究/Garcia A//Neurology.-1998,50(4).-1061～1067湘医图9926545 药物的选择和治疗实际:败血症,化脓性髓膜炎[日]/远藤晃彦//周产医.-1998,28(10).-1291～1294 友谊医9926546 脑室周围白质软化症(PVL)的危机管理[日]/小保内俊雅//周产医.-1998,28(9).-1183～1188

Roles of Long-chain Acyl Coenzyme A Synthetase in Absorption and Transport of Fatty Acid

Long-chain acyl coenzyme A synthetase(ACSL) is a member of the synthetase family encoded by a multigene family;it plays an important role in the absorption and transport of fatty acid.Here we review the roles of ACSL in the regulating absorption and transport of fatty acid,as well as the connection between ACSL and some metabolic diseases.

Genetic determination of irritable bowel syndrome

Irritable bowel syndrome （IBS） is a common functional gastrointestinal disorder. According to the Rome m criteria, IBS is defined as recurrent abdominal pain or discomfort for at least 3 d per month during the previous 3 mo associated with two or more of the following symptoms： improvement with defecation, onset associated with a change in the frequency of stool and/or onset associated with a change in form or appearance of stool. There is growing evidence regarding the genetic contribution in IBS, however the precise etiology of IBS is still unknown. The evaluation of the genetic influence is based on twin studies, familial aggregation and genetic epidemiological investigations. Most studies showed a concordance for IBS significantly greater in monozygotic than in dizygotic twins. The majority of the studies have shown that familial aggregation may represent exposures to a similar environment, as well as the influence of genetic factors. Whereas no specific gene has been identified in association with IBS, recent studies have noticed the importance of polymorphisms in the promoter region of the serotonin reuptake transporter gene, G-protein beta 3 subunit gene （C825T）, cholecystokinin receptor （CCKAR gene 779T〉C）, and high-producer tumor necrosis factor genotype. Further studies are necessary to determine how genetic factors influence the clinical manifestations and therapeutical response in IBS patients.

G-protein beta 3 subunit polymorphisms and essential hypertension： a case-control association study in northern Han Chinese

Objective To explore the association between the three polymorphisms [ C825T, C1429T and G（-350）A] of the gene encoding the G protein beta 3 subunit （GNB3） and hypertension by performing a case-control study in the northern Han Chinese population. Methods We recnaited 731 hypertensive patients and 673 control subjects （the calculated power value was 〉 0.8）. Genotyping was performed to identify C825T, C1429T and G（-350）A polymorphisms using the TaqMan assay. Comparisons of allelic and genotypic frequencies between cases and controls were made by using the chi-square test. Logistic regression analyses were performed to investigate the relationships between the three polymorphisms of GNB3 gene under different genetic models （additive, dominant and recessive models）. Results The genotype dis- tribution and allele frequencies of C825T, C1429T and G（-350）A polymorphisms did not differ significantly between hypertensive patients and control subjects, either when the full sample was assessed, or when the sample was stratified by gender. No significant association was observed between C825T, C 1429T and G（-350）A polymorphisms and the risk of essential hypertension in any genetic model. Linkage dis- equilibrium was only detected between C825T and C 1429T polymorphisms. Haplotype analyses observed that none of the three estimated haplotypes significantly increased the risk of hypertension. Conclusions Our study suggested that the GNB3 gene polymorphisms [C825T, C 1429T and G（-350）A] were not significantly associated with essential hypertension in northern Han Chinese population.

LBP and CD14 polymorphisms correlate with increased colorectal carcinoma risk in Han Chinese

AIM: To explore the associations of polymorphisms of lipopolysaccharide binding protein (LBP), cluster of differentiation 14 (CD14), toll-like receptor 4 (TLR-4), interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) with the colorectal carcinoma (CRC) risk in Han Chinese. METHODS: Polymorphisms of LBP (rs1739654, rs223 2596, rs2232618), CD14 (rs77083413, rs4914), TLR-4 (rs5030719), IL-6 (rs13306435) and TNF-α (rs35131721) were genotyped in 479 cases of sporadic colorectal carcinoma and 486 healthy controls of Han Chinese in a case-control study. Single-nucleotide polymorphisms (SNPs) between cases and controls were analyzed by unconditional logistic regression. RESULTS: GA and GG genotypes of LBP rs2232596 were associated with a significantly increased risk ofCRC [odds ratio (OR) = 1.51, 95% confidence interval (CI) 1.15-1.99, P = 0.003; OR = 2.49, 95% CI 1.16-5.38, P = 0.016, respectively]. A similar association was also observed for the CG genotype of CD14 rs4914 (OR= 1.69, 95% CI 1.20-2.36, P = 0.002). In addition, a combination of polymorphisms in LBP rs2232596 and CD14 rs4914 led to a 3.4-fold increased risk of CRC (OR = 3.44, 95% CI 1.94-6.10, P = 0.000). CONCLUSION: This study highlights the LBP rs2232596 and CD14 rs4914 polymorphisms as biomarkers for elevated CRC susceptibility in the Chinese Han population.

Analysis of TLR4 and TLR2 polymorphisms in inflammatory bowel disease in a Guangxi Zhuang population

AIM: To study the polymorphisms of toll-like receptor 4 (TLR4) gene Asp299Gly, Thr399Ile and TLR2 gene Arg753Gln, Arg677Trp and susceptibility to inflammatory bowel disease (IBD) in the Zhuang population from Guangxi, China. METHODS: A case-control study was performed from February 2007 to October 2011 which included 146 Zhuang patients with IBD in the experimental group and 164 healthy Zhuang subjects who acted as the control group. All patients and healthy subjects were from the Guangxi Zhuang Autonomous Region of China. Genomic DNA was extracted from intestinal tissue by the phenol chloroform method. TLR4 gene Asp299Gly, Thr399Ile and TLR2 gene Arg753Gln, Arg677Trp were amplified by polymerase chain reaction (PCR), and then detected by PCR-restriction fragment length polymorphism (RFLP). RESULTS: The TLR4 gene Asp299Gly was digested using Nco Ⅰ restriction enzyme, and a single band of 249 bp was observed which showed that it was a wild type (AA). The TLR4 gene Thr399Ile was digested using Hinf Ⅰrestriction enzyme and only the wild type (CC) was detected. In addition, the TLR2 gene Arg-677Trp was digested using Aci Ⅰ restriction enzyme and only the wild type (CC) was detected. The TLR2 gene Arg753Gln was digested using Pst Ⅰ restriction enzyme. Only the wild type (GG) as a single band of 254 bp was observed during RFLP. Overall, no heterozygous or homozygous single nucleotide polymorphism mutations were found in patients with Crohn's disease and ulcerative colitis both in the TLR4 gene Asp299Gly, Thr399Ile and the TLR2 gene Arg677Trp, Arg753Gln in the Zhuang population from the Guangxi Zhuang Autonomous Region of China. CONCLUSION: The TLR4 gene Asp299Gly, Thr399Ile and TLR2 gene Arg753Gln, Arg677Trp polymorphisms may not be associated with IBD in the Zhuang population from the Guangxi Zhuang Autonomous Region of China.

应用O-抗原基因簇特异PCR对假结核耶尔森菌分型

目的：依据假结核耶尔森菌O-抗原基因簇多态性，应用PCR基因分型方法替代传统的血清分型方法，时本室保存的31株假结核耶尔森菌进行分型研究。方法：通过比较假结核耶尔森菌与已知血清型假结核耶尔森菌标准参考株O-抗原基因簇的差异，确定实验株的血清型。结果：应用PCR基因分型方法替代传统的血清分型方法，对本室保存的31株假结核耶尔森菌进行了成功的血清分型，血清组Ⅰ-Ⅴ与血清型0：1～0：5存在着对应关系。结论：应用PCR方法对假结核耶尔森菌进行血清分型，方法简单、迅速，同时不需要制备特异抗血清，是一种理想的血清分型替代方法。

胃癌组织中干细胞标志物性别决定相关基因簇2以及外周血树突细胞功能对胃癌预后的影响

目的分析胃癌组织中干细胞标志物性别决定相关基因簇2(Sox2)以及外周血树突细胞(DC)功能对预后的影响,为胃癌患者预后的早期评估提供参考依据。方法选取70例胃癌患者及50名健康受试者,使用实时荧光定量多重聚合酶链反应(PCR)检测胃癌组织、正常胃黏膜Sox2表达,并对其外周血DC进行检测。比较两组受试者及不同病理指标胃癌患者间上述指标间差异,应用受试者工作特征曲线(ROC)分析Sox2及DC对患者预后的诊断价值。应用t检验和ROC曲线分析进行统计分析。结果胃癌组织Sox2 mRNA相对表达量显著低于正常胃黏膜组织,差异有统计学意义(P=0.002);患者组外周血DC/PBMC显著低于正常组,差异有统计学意义(P=0.013)。不同TNM分期、细胞分化程度、肿瘤浸润深度、淋巴结转移状态胃癌患者肿瘤组织Sox2、DC计数、DC/PBMC表达比较,差异有统计学意义(P=0.006,0.011,0.007,0.000)。Sox2 mRNA表达和DC计数降低预测患者3年病死的AUC分别为0.877、0.935,P=0.000,0.000,均可有效预测患者预后。结论胃癌组织中Sox2表达、DC计数降低是胃癌患者预后不良的预测因子。