小麦显性矮秆基因复等位多态特性研究

世界小麦矮化育种主要使用隐性矮源.在普通小麦中发现的显性矮源均因导致植株极度矮化(20～55cm)而未能在小麦育种中广泛应用.笔者发现,将显性矮源矮变1号(4DS携带Rht10,25～30 cm)及矮苏3(4BS携带Rht3,55 cm)的原种大群体种植或施以诱变因子并将其与中、高秆的小麦品种杂交、回交,从其分离世代的大群体中,均可选择到一些株高呈不同程度提升的稳定的突变株系.采用近等基因系法对不同株高突变衍生系的研究表明:其提升的株高真实遗传,各自均携带一个不同株高的半显性矮秆基因,随突变衍生系株高的提升,近等基因系的产量性状显著优化.采用标志基因测交法以及生理生化遗传标记对突变衍生系携带的不同株高的半显性矮秆基因重新进行了基因定位,确认它们分别与Rht1 0及Rht3的座位相同,因而均是其突变衍生的复等位基因.提出显性矮秆基因具有'复等位多态特性',即极度矮化的显性矮秆基因容易突变为一群株高提高程度不同的、可以达到小麦育种理想株高的半显性矮秆复等位基因.

有限长圆柱状催化剂内扩散效率因子多态研究(Ⅱ)——多态特性

本文(I)报开发的研究方法和量级分析的思想探讨了圆柱状催化剂内发生强放热不可逆反应时的多态特性,针对多态区域的预测,本文以粒内浓度分布为出发点,提出了一种锥体简化方法,用此法所得预测值与实际计算值吻合良好。

有限长圆柱状催化剂内扩散效率因子多态研究(Ⅰ)——研究方法的开发

对有限长圆柱状催化剂内发生强放热反应时的多态特性,本文提出了较为完整的研究方法,包括:(1)将有限北方法与非线性连续性算法及奇性理论结合,开发了有限元全区域分析方法,作为研究非线性偏微分方程多解的基本工具;(2)在高数值效率因子计算中,改进传统方法,开发了高效、准确的新方法,通过实验数据检验了有限元全区域分析方法的可靠性,结果令人满意。

JAVA语言中的多态

多态在任何一门面向对象语言中都是一个非常重要的特性.面向对象的很多优点和灵活性都因它而存在,所以对于Java语言,其多态特性尤为重要.

面向电力系统暂态稳定分析的双馈风电机组动态模型

大规模风电并网对系统暂态和中长期稳定都有重要影响,提出能够用于电力系统稳定分析的风电机组、风电场简化模型对于深入研究风电与电网暂态互动是十分重要的。目前WECC、IEC、GE等研究机构已提出了各自的风电机组、风电场标准化通用模型,但模型均较为简化,对于双馈风电机组建模没有考虑暂态过程中的变流器闭锁和转子crowbar投切等机制,导致外特性准确性不足,使得暂态稳定分析结果过于乐观。针对该不足,文中按多时间尺度模型简化原理,系统地推导双馈风电机组气动、机械及电气部分的简化模型,并着重探讨电网电压扰动强度与风电变流器"电流源–电压源–无源阻性"3种状态特性的关系,从而导出能直接用于暂态稳定分析的简化模型和Crowbar投入、切出代数判据,在保证仿真效率的同时提高了模型暂态特性的精度。最后,通过与详细模型的仿真对比及与机组实验结果的对照,验证所提简化模型的有效性。

小麦半显性创新矮源“西南02～10”

1 小麦显性矮秆基因"复等位多态特性"的发现 小麦矮化育种导致了"绿色革命",矮秆基因已成为现代小麦育种的主要遗传支撑.但当今世界小麦育种仍局限于使用隐性矮源,已发现、报道的显性矮源均因植株极度矮化而未能在小麦育种中得到广泛的应用.这不但存在着"遗传脆弱性的潜在威胁",而且由于现代小麦育种的理想株高仍然呈逐渐下降的趋势,缺少株高在70～80 cm左右的单基因矮源.因此,开拓小麦育种新矮源的研究具有重要的意义.西南农业大学麦类研究室经多年研究,采用矮变1号(4DS携带Rht10)、矮苏3(4BS携带Rht3)、奥尔森(待定位)、农大3222(5A携带Rht12)、农大3338(2AS携带Rht21)等显性矮源,通过杂交育种、辐射诱变、天然突变等方式选育出了西南02～10等一批株高呈不同程度提升的半显性创新矮源.

Screening of a Novel Bioflocculant-producing Strain and Research on Its Flocculation

[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.

Relationship between Crohn’s disease, infection with Mycobacterium avium subspecies paratuberculosis and SLC11A1 gene polymorphisms in Sardinian patients

AIM： To study the association between Crohn＇s disease （CD）, Mycobacterium avium subspecies paratubercuolsis （MAP）, and genetic factors by examining the role of natural resistance-associated macrophage protein 1 （NRAMP1） gene polymorphisms （now SLCllA1） in Sardinian patients with CD and controls. METHODS： Thirty-seven CD patients and 34 controls with no inflammatory bowel disease （IBD） were recruited at the University of Sassari after giving written consent. Six SCL11A1 polymorphisms previously reported to be the most significantly associated with IBD were searched. M, pafatubefculosis was identified by IS900 PCR and sequencing. Logistic regression was used to calculate odds ratios （OR） for the associations among CD, presence of MAP, and 6 loci described above.＇ RESULTS： For the first time, a strong association was observed between polymorphisms at NRAMP1 locus 823C/T and CD. While CD was strongly associated with both NRAMP1 and MAP, NRAMP1 polymorphisms and MAP themselves were not correlated. CONCLUSION： Combined with previous work on the NOD2/CARD15 gene, it is clear that the interplay of genetic, infectious, and immunologic factors in the etiology of CD is complex.

Study on the sex-related AFLP marker of the Yangtze finless porpoise

The sex-related molecular marker of the Yangtze finless porpoise was screened using Amplified Fragment Length Polymorphism (AFLP) technique combined with the bulked segregant analysis. Totally 36 AFLP primer combinations were used to detect the genome DNA bulks of the female and male porpoises, and one sex-related AFLP marker was finally obtained. The marker can be applied to sex identification, and provides a base for further cloning of sex-related genes and analyzing of Y chromosome haplotypes of the Yangtze finless porpoise.

Relationship between Physical and Chemical Soil Attributes and Plant Species Diversity in Tropical Mountain Ecosystems from Brazil

Although the high diversity of plant species in the rupestrian fields has been primarily attributed to the existence of a set of distinct habitats, few studies support this assertion. The present study aimed to further investigate the relationship between physical and chemical attributes of soils with the diversity of plant species in this unique ecosystem. The rupestrian field is a unique vegetation formation that covers some of the southeastern Brazilian mountains in the transition of the Atlantic rain forest and the Cerrado(savanna). Different habitats occur according to soil characteristics(e.g., presence of rocks, sand, fertility, hydrology, etc.). These attributes ultimately influence the vegetation that is highly adapted to the harsh edaphic and climatic mountain conditions. Five distinct habitats were studied by us: rocky outcrops, peat bogs, sandy bogs, quartz gravel fields, and "cerrado"(savanna). A floristic survey indicated that four families are found at greater frequency: Poaceae, Asteraceae, Cyperaceae, and Leguminosae. The greatest diversity of plant species was found in the rocky outcrops habitat, followed by cerrado, peat bog, quartz gravel grassland, and sandy bogs, respectively. The main difference in the floristic composition among these habitats was related to the dominant species. Trachypogon spicatus(Poaceae) was the most dominant species in the rocky outcrops, Axonopus siccus(Poaceae) in the peat bogs, Lagenocarpus rigidus(Cyperaceae) in the sandy bogs, Schizachyrium tenerum(Poaceae) in the cerrado, while Vellozia sp. 8(Velloziaceae) dominated the vegetation in the quartz gravel grassland. This study demonstrated that physical and chemical soil properties strongly related the diversity of plant species occurring in the different habitats of rupestrian fields.

Panax ginseng-specific sequence characterized amplified region (SCAR) marker for testing medicinal products

To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA （RAPD） was performed using 120 random primers. Of the successful amplicons obtained, the Panax ginseng-specific RAPD marker C-12 was cloned into a TA vector and sequenced （Genl3ank access number KU553472）. Based on the sequence analysis results, a pair of primers specific to C-12 was designed. Finally, a SCAR marker-based identification system for Panax ginseng was developed after optimization of the reaction conditions. Using this method, two positive bands were stably observed at 300 bp and 130 bp in 33 batches of Panax ginseng samples tested, while negative results were obtained for another 101 batches of samples, including Panax quinquefolium, Panax notoginseng, adulterants, and other medicinal herbs. Thus, we successfully developed a PCR-based method for rapid and effective identification of Panax ginseng, which can be effectively used for the protection and utilization of germplasm resources and identification of the origins of Panax ginseng samples.

Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set

Buffaloes （Bubalus bubalis） are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific （cattle） markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three PCR mixes were finally developed with the 11 markers that succeed in amplify and were polymorphic （58%）. The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. As it was expected, Brazilian buffalo variability was lower than the previously reported from the domestication centres （China and India）, but higher than the seriously selected European populations. The exclusion power calculated for the eleven markers in Brazilian buffalo was 0.9999999996, this allows its use in DNA based traceability.

Survey of Plant Community Characteristics in Bamenwan Mangrove of Wenchang City

According to natural conditions and vegetation distribution characteristics of Wenchang Bamenwan Mangrove Forest, sample plots were set up to investigate plant community characteristics in this area, and to explore the diversity characteristics of different subareas and calculate importance values of various tree species in the study area. According to the investigation, there are 48 plant species of 42 genera and 28 families in Bamenwan Mangrove Forest, including 29 tree species of 24 genera and 17 families. Among the tree species, those on the top 5 in the importance value list are Hibiscus tiliaceus, Bruguiera sexangula, Lumnitzera littorea, Rhizophora apiculata, Xylocarpus granatum. There are 3 shrub species of 3 genera and 3 families, including Atalantia buxifolia, Lantana camara and Streblus asper. And there are 16 herbal species of 16 genera and 11 families, including Acrostichum aureum, Vernonia cinerea, Acanthus ilicifolius, Alocasia macrorrhiza, Synedrel a nodiflora, Gynura japonica and etc.. Diversity of the mangrove in different areas varies greatly, Shannon-Wiener index and richness index of semi-mangrove forest are both higher than those of other 3 mangrove forests. Bamenwan Mangrove Forest is characterized by diversified species and complex structures, but the community diversity is unevenly distributed because of the interventions from many factors. More human efforts are suggested to enhance the mangrove protection, so as to enrich the community diversity.

Research on dynamic characteristics model test scheme for middle pylon of multi-pylon multi-span suspension bridges

Multi-pylon multi-span suspension bridge is a new type super flexible structure system, and the rigidity design of middle pylon is one of the main difficult technical issues. Due to the requirements of longitudinal rigidity, the structural form and the corresponding foundation type of middle pylon are different from those of the ordinary steel pylon, and the complicated dynamic characteristics make the calculation quite difficult. In this article, exploration has been made in selection of similarity ratio and model materials, section simulation, restriction conditions simulation, fixing of mass blocks, fabrication scheme and testing method by taking into account different construction and working conditions such as restriction conditions and working environment of a three-pylon suspension bridge, to conduct the test experimental design of the dynamic behavior of the middle pylon, with the purpose to reveal its dynamic characteristics and make comparison and analysis with theoretical assumptions, to provide basis for anti-wind and anti-seismic design and reference for the design and research of three-pylon two-span suspension bridges in the future.

Spatial Correlation and Ecological Characteristics Analysis of Man-agement Area for Biodiversity Conservation and Relevant Regionalization

Formulation of different ecological zone plans according to the corresponding protection targets and the necessity of proper conservation policy is one of the measures to achieve the goal of ecological conservation in China.In order to clarify the interrelation among key ecological zone plans,this paper carried out the research on spatial relation of priority areas of biodiversity conservation and three key ecological areas(key ecological function areas,key regions of ecological service function,national nature reserves)and the research on ecological conditions,based on multi-scale ecological spatial theme information,which incorporates elements like ecological quality and type,and by the aid of spatial information analysis and GIS modeling.The results showed a contrastively fine spatial consistency with 68.8%of priority areas of biodiversity conservation overlapping with three key ecological areas.Although the environment in priority areas of biodiversity conservation were in good conditions,protection pressure is also increasing,powerful supervision and protection should not be ignored.The environmental conditions in the overlapping areas,as a whole,were superior to those in the non-overlapping areas.Since two areas have different characteristics,targeted protection measures should be formulated based on this difference,which will be very important for biodiversity conservation in priority areas of biodiversity conservation.

Analytical Results of Eigenstates and Eigenenergies for Three Kinds of Models Describing N-mode Multiphoton Process

We obtain. the exact analytical results of all the eigenvalues and eigenstates for three kinds of models describing N-mode multiphoton process without using the assumption of the Bethe ansatz. The exact analytical results of all the eigenstates and eigenvalues are in terms of a parameter lambda for three kinds of models describing N-mode multiphoton process. The parameter is shown to be determined by the roots of a polynomial and is solvable analytically or numerically. Moreover, these three kinds of models can be processed with the same procedure.

The Evolution of Stinging Hairs and Other Characters in Nettle Family

Urticaceae Juss.is a large cosmopolitan family and taxonomically difficult group,partly because it encompasses a broad range of morphological diversity and many of the diagnostic characters（e.g.flower,achene.stipule,bract）require a microscope for accurate determination.Meanwhile,most Uriiceae species have stinging hairs which make them more difficult to collect and identify.As a result,the infra-familial classification of Urticaceae has been controversial for more than a century.A research group led by Prof.

Effect of interleukin-18 polymorphisms-607 and -137 on clinical characteristics of prostate cancer patients

Objective: The aim of this study was to determine whether the presence of IL-18 polymorphisms -137 G/C and -607 A/C was associated with grade, clinical stage, and survival in patients with prostate cancer. Methods: The study cohort included 126 patients with prostate cancer. Control group consisted of 125 samples from Chinese population. Genomic DNA was extracted from EDTA-anticoagulated peripheral blood leukocytes by the salting-out method. The genotyping of the two IL-18 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Results: The studied IL-18 gene polymorphisms did not influence susceptibility to prostate cancer in the analyzed group of patients (IL-18-607, P = 0.342; IL-18-137 P = 0.715) but may contribute to disease onset and aggressiveness. IL-18-607 CC genotype was significantly associated with higher tumor grade (P = 0.025) and stage (P = 0.001). IL-18-137 GG genotype was correlated with higher tumor grade (P = 0.018) and stage (P = 0.007). The Cox proportional hazard model showed that tuumor grade and stage grouping were independent prognostic factors but IL-18 polymorphism was not. Polymorphism variants in the IL-18 gene (IL-18-607 and IL-18-137) may be associated with a worse prognosis for prostate cancer. Conclusion: High levels of IL-18 production may play a major role in the growth, invasion and metastasis of prostate cancer.

The relationship between the ratio of second fingers to fourth fingers （2D：4D） and the inheritance of athletic ability

This paper summarizes the ratio of 2D and 4D to the inheritance of athletic ability： 2D： 4D has been stereotypes before birth, and the mother＇s uterus testosterone concentration there is a negative correlation between the relationship, Testosterone concentration affects the development of the right hemisphere and spatial visual ability of the brain, thus affecting athletic ability. The mitochondrial genome （mtDNA） polymorphism is directly related to motor activity and has a high maternal genetic trait. Athlete mothers have higher testosterone, and their children have athletic advantages. 2D： 4D and mtDNA have good genetic markers, which are of great significance and prospect for the study of their inheritance of motor ability.

Optimality conditions for sparse nonlinear programming

The sparse nonlinear programming （SNP） is to minimize a general continuously differentiable func- tion subject to sparsity, nonlinear equality and inequality constraints. We first define two restricted constraint qualifications and show how these constraint qualifications can be applied to obtain the decomposition properties of the Frechet, Mordukhovich and Clarke normal cones to the sparsity constrained feasible set. Based on the decomposition properties of the normal cones, we then present and analyze three classes of Karush-Kuhn- Tucker （KKT） conditions for the SNP. At last, we establish the second-order necessary optimality condition and sufficient optimality condition for the SNP.