GB14048.5按钮类多样品驱动实验系统的研究

GB14048.5是针对低压控制电路电器和开关元件、机电式控制电路电器进行相关实验的国家检测实验标准。其中涉及的按钮、微动开关等实验过程都会有多个不同型号同实验参数的样品要进行实验。样品的实验步骤复杂、实验次数多达数千次,因此实验效率极低。为了提高实验效率和精度,提高实验过程的监测保护能力,本文通过反复研究和实践,开发出多样品实验控制系统。

多样品同时萃取-气相色谱法测定食品中山梨酸和苯甲酸

研究了多样品同时萃取/气相色谱法对食品中山梨酸和苯甲酸的快速测定。以醋酸乙酯+正己烷(1+1)混合溶剂对样品和标准系列进行一次性萃取,用SE-30宽口径毛细柱作分析柱对萃取相进行分析。方法条件下,样品中山梨酸和苯甲酸的检出限为1mg/kg,线性范围25-500 mg/kg,回收率93.2%、102.4%,相对标准偏差(RSD)为1.9-4.1%。方法操作简便快捷易于控制,使用试剂少成本低,用于批量样品的山梨酸和苯甲酸快速测定获得良好效果。

超灵敏小型回旋加速器质谱计推拉式垂直型多样品装置的研制

介绍了推拉式垂直型多样品装置的研制、装置的结构与特点,技术规范与性能精度、工作过程与流程。

超灵敏小型回旋加速器质谱计旋转式垂直型多样品装置的机械设计

介绍旋转式垂直型多样品装置机械设计,包括装置的结构,技术规范和技术要求,有关主要零部件技术设计,以及本多样品装置与美国NEC公司制造的多样品装置结构上的比较。

一种多样品臂扫频光学相干层析系统

报道了一种多样品臂扫频光学相干层析(OCT)系统,使用光开关将多路样品臂接入同一个扫频OCT成像系统,通过光开关的快速切换,实现了多路同时实时成像。设计并展示一个双样品臂的成像系统,两路成像性能均与典型扫频OCT系统相当;通过两路实时成像结果相结合,在仅使用少量额外硬件的情况下,实现了对成像视场的成倍扩展。

超灵敏小型回旋加速器质谱计垂直旋转型多样品装置自动控制系统

本文介绍用于我所超灵敏小型回旋加速器质谱计垂直旋转型多样品装置自动控制系统原理及应用 ,该系统亦可用于其他多样品自动装置。

超灵敏小型回旋加速器质谱计旋转式垂直型多样品装置的自动控制系统

介绍用于超灵敏小型回旋加速器质谱计旋转式垂直型多样品装置自动控制系统原理及应用,该系统亦可用于其他多样品自动装置。

多样品组织研磨仪在提取菊科植物各组织中总RNA的应用

通过多样品组织研磨仪处理菊科植物组织,从其根、茎、叶和花蕾中提取纯度高、完整性好的RNA。总RNA浓度与纯度通过分光光度计检测,总RNA的质量与完整性通过琼脂糖凝胶电泳检测。通过与传统研磨破碎方法处理得到的结果进行对比,发现多样品组织研磨仪处理的菊科植物组织在确保所提取的RNA高质量和完整性的前提下,能够从相应的植物组织中获得更高含量的RNA。

采用多样品引入系统HG-ICP-AES法测定汽轮机材料中砷、铋、锑元素

在多样品引入系统(MSIS)中,选择砷谱线波长193.696nm,铋谱线波长223.061nm,锑谱线波长217.582nm作为分析线,用试验方法探讨了介质酸度、硼氢化钾浓度、射频发生器(RF)功率等对最终测定结果的影响,从而实现了使用电感耦合等离子体发射光谱法测定汽轮机用钢铁材料中微量元素砷、铋、锑的过程。在选定的仪器工作条件下,各元素的校准曲线线性相关因数均大于0.9990,各元素的检出限为0.00036%~0.00059%。将该试验方法用于标准样品中砷、铋、锑的测定,结果的相对标准偏差(RSD,n=6)为2.66%~8.21%,测定值与认定值基本吻合。研究成果可为汽轮机钢铁材料中微量元素砷、铋、锑化学成分测试提供参考。

多样品法确定类金刚石薄膜的光学常数与厚度

采用乙烷气体辉光放电法在单晶Si衬底上制备了名义厚度分别为75,150和250nm的类金刚石碳(DLC)薄膜,除沉积时间外其他工艺参数完全一致。使用可变入射角光谱型椭偏仪(VASE)测量了380～1700nm波段的椭偏谱。该研究发现,对单一DLC样品的椭偏数据进行分析时,一定的范围内,假定不同的薄膜厚度均可以得到非常好的拟合结果。结果表明,采用单样品椭偏法拟合时,厚度与光学常数呈现出强烈的关联性,无法快速获得准确的结果。采用多样品椭偏法,对三个样品建立相同的物理模型,假定他们的光学常数相同,进行数据拟合。分析发现该方法可以快速、简便地获得精确的折射率、消光系数以及厚度值。经过检验,结果具有非常好的唯一性。

材料高通量试验及表征技术研究进展

随着美国于2011年率先提出材料基因组计划(materials genome initiative,MGI),为了解决传统材料研发“试错法”耗时长、成本高、效率低的问题,加速新材料的研发及应用,降低材料研发成本,近年来世界各国均开展了大量材料基因组计划相关研究。作为材料基因组计划的三大重要组成部分之一,材料高通量实验(试验及表征)技术已成为各国研究学者的重点关注对象。首先对材料高通量试验设计原理、实现思路及发展现状进行了梳理,分析了高通量试验在金属、非金属等材料体系制备及试验评价中的发展;同时总结了材料高通量表征技术的发展,根据表征参量不同,分析介绍了多种高通量表征技术;最后根据材料高通量试验及表征技术现状,对当前高通量试验及表征技术进行了总结及展望。

椭偏谱法测量类金刚石薄膜的质量

应用椭偏光谱法研究了一系列不含氢DLC样品,讨论了样品制备与测量、模型的建立及多样品分析法和椭偏数据拟合,表明椭偏光谱法可以确定DLC膜的厚度,并可反映出sp3成份百分比变化与制备时偏置电压的关系.

RAPD Analysis of 33 Varieties of Banana

RAPD assessment of genetic variations in 33 varieties of banana (Musa nana Lour.) was carried out. Eighteen primers were screened from 249 10 bp arbitrary primers, and a total of 192 DNA bands were amplified, among which 183 (95.31%) were polymorphic. The average number of DNA bands amplified by each primer was 10.67, and the average genetic distance among 33 varieties was 0.3412. Based on UPGMA cluster analysis of 192 DNA bands amplified by 18 primers a DNA molecular dendrogram was established for 33 varieties of banana in China, which divided the 33 varieties into four groups: group A, B, C and D. Group A included 20 varieties, group B included 5 varieties, group C included 2 varieties and group D included 6 varieties. Group A could be divided into 3 sub-groups: A1, A2 and A3. The molecular foundation of genetic diversity of banana was also explored.

Genetic Diversity of Maize (Zea mays L.) Landraces from Southwest China Based on SSR Data

Genetic diversity of 54 maize landraces from southwest China was tested using bulk DNA samples and 42 microsatellite （SSR） loci distributed on 10 chromosomes of maize. A total of 256 alleles were detected among the landraces. At each locus, the number of alleles varied from 2 to 9, with an average of 6.1. On the basis of the genetic similarity coefficients, clustering analysis separated the landraces into four groups. The landraces collected from the same region were mostly grouped together. To reveal the genetic structure and genetic diversity within landraces, 165 individuals from 11 landraces were analyzed. Individual DNA samples proved to be superior to bulk DNA samples in identifying genetic diversity of landraces. A total of 330 alleles were detected in the 11 landraces. According to the results of the individual DNA sampling analysis, estimates of the mean number of alleles ‘A’, the effective allelic number ‘Ae’, the observed heterozygosity ‘Ho’, and expected heterozygosity ‘He’ were 7.86, 3.90, 0.69, and 0.37, respectively. An obvious genetic deviation from Hardy-Weinberg expectation was observed both among and within landraces and a considerable genetic variation was revealed within rather than among landraces. In addition, genetic diversity of landraces was greater in Sichuan than in the other three regions. It can be concluded that maize landraces in southwest China were initially introduced to Sichuan and from there to adjacent areas.

Genetic Diversity of Gli-1, Gli-2 and Glu-1 Alleles in Sichuan Wheat Landraces

Genetic diversity at Gli_1, Gli_2 and Glu_1 loci was investigated in 89 Sichuan wheat (Triticum aestivum L.) landraces by using acid polyacrylamide gel electrophoresis (APAGE) and SDS_PAGE. In these landraces, a total of 32 gliadin and 3 high_molecular_weight (HMW) glutenin patterns were observed. In total, 14, 15 and 5 alleles were identified at Gli_1, Gli_2 and Glu_1, respectively. At each locus, the alleles in higher frequency were Gli_A1a (89%), Gli_B1h (46%), Gli_D1a (65%), Gli_A2a (64%), Gli_B2j (45%), Gli_D2a (48%), Glu_A1c (99%), Glu_B1b (99%) and Glu_D1a (100%). The Nei's genetic variation index (H) of Sichuan wheat landraces was 0.370?6, varying from 0 to 0.708?7. The highest genetic diversity was found at Gli_B2 locus, while the lowest was found at Glu_D1. The genetic diversity at Gli loci was higher than that of Glu_1 loci among these landraces, but it was much lower than that of modern wheat cultivars. These results indicated a narrow genetic base of Sichuan wheat landraces. In this study, “Chengdu_guangtou” had the identical gliadin and HMW_glutenin patterns with “Chinese Spring”, further supporting the proposal that “Chinese Spring” is a strain of “Chengdu_guangtou”.

Genetic Analysis of Six Endangered Local Duck Populations in China Based on Microsatellite Markers

Seventeen microsatellite loci were used to assess the genetic variation and population structure of six endangered duck populations in China. PIC, heterozygosity with two estimators of genetic differentiation （FST and GST）, and Nei＇s standard genetic distance were evaluated. The results showed that these six endangered duck populations showed high polymorphism. The propor- tion of inter-population subdivision among the six duck populations ranged between 17.0 and 14.7%. The average heterozygosity was 0.706, 0.702, 0.691, 0.676, 0.604, and 0.660 in the Gaoyou, Liancheng, Jinding, Beijing, Shaoxing, and Jianchang ducks, respectively. The average PIC ranged from 0.561 to 0.663. Finally, the results showed higher genetic diversity and difference above the levels of genetic variation among all the populations.

Genetic Diversity Analysis and Constructing Core Collection Based on Phenotypes in Cotton

In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collection by phenotypes.Phenotyping and statistic analysis on 9 traits of 92 accessions of cotton germplasm resource from three species（Gossypium hirsutum L.,Gossypium barbadence L.and Gossypium arboreum L.）were conducted.And the statistics（variation coefficient,proportion of special accessions and Shannon-Weaver information index）indicated that initial collection had abundant phenotypic diversity;software NTSYS-pc and the unweighted pair group method of arithmetic（UPGMA）were used for the cluster analysis on genetic similarity coefficient and genetic distance matrix,and the result showed that the genetic relationship among accessions was highly consistent with the pedigree;22 accessions of core collection were selected by software QGAStation,four statistics,such as variance difference percentage（VD%）,mean difference percentage（MD%）,coincidence rate（CR%）and variable rate（VR%）,showed that the genetic diversity of core collection was approximately equal to the initial collection.The results of genetic diversity analysis based on phenotypic data and sampling of core collection would provide reference for breeding projects and basic research.

Genetic Diversity Analysis of Three Guizhou Local Pig Breeds Using Microsatellite DNA Markers

[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations （FAO） and international Society for Animal Genetics （ISAG）, six pairs （S0155, SW240, IGF1, SW951, SW857, SW24） were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci （PIC 〉0.5）. The Nei＇s standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.

企业经营的“法门”——介绍镇江市铸造阀门厂

镇江市铸造阀门厂是江苏省阀门协会成员厂,一九七○年以来,尤其是通过近几年来的改革和新产品开发等方面的工作,生产迅速发展,已由过去单一生产铸造截止阀,逐步发展到能生产铸铁、铸钢和不锈钢中低压闸阀、截止阀和球阀等多样品种,规格达207种之多,