该文提出了一种全新的高速连接器时域模型MMTL(Multi-segment Multiple Transmission Line model)。该模型克服了MLC模型不稳定和仿真效率低的缺点,具有直观、精度可控、便于优化等优点。实验结果表明,该文所建模型精度高,与实际测量的...该文提出了一种全新的高速连接器时域模型MMTL(Multi-segment Multiple Transmission Line model)。该模型克服了MLC模型不稳定和仿真效率低的缺点,具有直观、精度可控、便于优化等优点。实验结果表明,该文所建模型精度高,与实际测量的最大误差小。展开更多
The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as ...The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite.展开更多
The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 mate...The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 maerials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocks for P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.展开更多
The development of modem information technology brings opportunities to vocational English teaching reform. Teachers use the advantages ＆modem information technology can create a good teaching situation, optimize the...The development of modem information technology brings opportunities to vocational English teaching reform. Teachers use the advantages ＆modem information technology can create a good teaching situation, optimize the classroom teaching process, cultivate student＇ s self-learning ability, and improve the effectiveness of vocational English classroom teaching. The purpose of college English teaching is to enable students to master certain basic knowledge and skills in English, with some listening, speaking, reading, writing, translation ability, a simple verbal and written communication in daily activities and foreign business activities. Modem information technology provides a strong tecbaaical support to achieve this goal. Multimedia teaching methods widely used in teaching activities to vocational English class have brought a series of profound changes. Teachers using modem educational teclmology to fully develop learning resources, build new teaching model, which make vocational English classroom full of vigor and vitality.展开更多
Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantifica...Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantification within both kinds of sludge were monitored by terminal restriction fragment length polymorphism （T-RFLP） and real-time PCR （RT-PCR） technique, respectively. The results showed that the COD removal of both kinds of sludge changed slightly when the ammonia removal efficiency decreased gradually with the ammonia concentration increased from 100 mg L^-1 to 500 mg L^-1 Furthermore, activated sludge demonstrated higher ammonia removal ability than that of aerobic granular sludge （10%- 16%）. As revealed by T-RFLP, activated sludge was of higher ammonia removal ability and more abounding bacterial diversity than that of aerobic granular sludge, suggesting that the bacterial diversity was probably relevant to the ammonia removal. The RT-PCR results indicated that the AOB population size of activated sludge and aerobic granular sludge were 2.80× 10^4-3.44× 10^4cells （g dried sludge）^-1 and 7.83×10^4-1.18×10^5cells （g dried sludge）^-1, respectively. There is no obvious positive correlation between the ammonia removal ability and number of AOB in both kinds of sludge.展开更多
基金Project (51374248) supported by the National Natural Science Foundation of ChinaProject (NCET-13-0595) supported by the Program for New Century Excellent Talents in University,China+3 种基金Project (2012AA061501) supported by the High-tech Research and Development Program of ChinaProject (2010CB630905) supported by the National Basic Research Program of ChinaProject (20120162120010) supported by the Research Fund for the Doctoral Program of Higher Education of ChinaProject (CSUZC2012020) supported by the Open-End Fund for the Valuable in Central South University,China
文摘The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite.
基金Supported by the National Key Project of Tenth-five Year Plan(2001BA707B)School Foundation Program of Henan Science and Technology University~~
文摘The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 maerials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysis was conducted with MVSP3.13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocks for P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.
文摘The development of modem information technology brings opportunities to vocational English teaching reform. Teachers use the advantages ＆modem information technology can create a good teaching situation, optimize the classroom teaching process, cultivate student＇ s self-learning ability, and improve the effectiveness of vocational English classroom teaching. The purpose of college English teaching is to enable students to master certain basic knowledge and skills in English, with some listening, speaking, reading, writing, translation ability, a simple verbal and written communication in daily activities and foreign business activities. Modem information technology provides a strong tecbaaical support to achieve this goal. Multimedia teaching methods widely used in teaching activities to vocational English class have brought a series of profound changes. Teachers using modem educational teclmology to fully develop learning resources, build new teaching model, which make vocational English classroom full of vigor and vitality.
基金Acknowledge： The study are supported by the Natural Science Foundation of Jiangsu Province （No. BK2005402） and National Natural Science Foundation （No. 30640018）.
文摘Effect of ammonia at different concentrations on aerobic granular sludge and activated sludge was investigated in this study. Meanwhile, bacterial diversity variation and ammonia oxidizing bacterium （AOB） quantification within both kinds of sludge were monitored by terminal restriction fragment length polymorphism （T-RFLP） and real-time PCR （RT-PCR） technique, respectively. The results showed that the COD removal of both kinds of sludge changed slightly when the ammonia removal efficiency decreased gradually with the ammonia concentration increased from 100 mg L^-1 to 500 mg L^-1 Furthermore, activated sludge demonstrated higher ammonia removal ability than that of aerobic granular sludge （10%- 16%）. As revealed by T-RFLP, activated sludge was of higher ammonia removal ability and more abounding bacterial diversity than that of aerobic granular sludge, suggesting that the bacterial diversity was probably relevant to the ammonia removal. The RT-PCR results indicated that the AOB population size of activated sludge and aerobic granular sludge were 2.80× 10^4-3.44× 10^4cells （g dried sludge）^-1 and 7.83×10^4-1.18×10^5cells （g dried sludge）^-1, respectively. There is no obvious positive correlation between the ammonia removal ability and number of AOB in both kinds of sludge.
