Objective speech quality is difficult to be measured without the input reference speech.Mapping methods using data mining are investigated and designed to improve the output-based speech quality assessment algorithm.T...Objective speech quality is difficult to be measured without the input reference speech.Mapping methods using data mining are investigated and designed to improve the output-based speech quality assessment algorithm.The degraded speech is firstly separated into three classes(unvoiced,voiced and silence),and then the consistency measurement between the degraded speech signal and the pre-trained reference model for each class is calculated and mapped to an objective speech quality score using data mining.Fuzzy Gaussian mixture model(GMM)is used to generate the artificial reference model trained on perceptual linear predictive(PLP)features.The mean opinion score(MOS)mapping methods including multivariate non-linear regression(MNLR),fuzzy neural network(FNN)and support vector regression(SVR)are designed and compared with the standard ITU-T P.563 method.Experimental results show that the assessment methods with data mining perform better than ITU-T P.563.Moreover,FNN and SVR are more efficient than MNLR,and FNN performs best with 14.50% increase in the correlation coefficient and 32.76% decrease in the root-mean-square MOS error.展开更多
In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside...In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.展开更多
A novel disposable paper-based bipolar electrode （BPE） array is fabricated for multiplexed electrochemiluminescence （ECL） detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each con...A novel disposable paper-based bipolar electrode （BPE） array is fabricated for multiplexed electrochemiluminescence （ECL） detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each consisting of six sensing cells and two reporting cells patterned using hydrophobic wax. A hairpin structure DNA assembled on the cathodes of BPEs hybridizes with Pt nanoparticles （NPs） labeled probe DNA in the presence of complementary target DNA. The introduction of Pt NPs catalyzes the reduction of dissolved 02 at cathodes and induces an enhanced ECL signal from Ru（bpy）32＋/tripropylamine （TPrA） at the anodes of BPEs. The dissolved 02 lost in reduction reaction could be promptly replenished due to the relatively large contact area of the paper-based cells with air, which ensures the stability of ECL signal. This obtained paper-based BPE array sensor showed excellent performances for the multiplexed analysis of the syphilis （Treponema pallidum） gene, the immunodeficiency virus gene （HIV） and hepatitis B virus gene （HBV）.展开更多
基金Projects(61001188,1161140319)supported by the National Natural Science Foundation of ChinaProject(2012ZX03001034)supported by the National Science and Technology Major ProjectProject(YETP1202)supported by Beijing Higher Education Young Elite Teacher Project,China
文摘Objective speech quality is difficult to be measured without the input reference speech.Mapping methods using data mining are investigated and designed to improve the output-based speech quality assessment algorithm.The degraded speech is firstly separated into three classes(unvoiced,voiced and silence),and then the consistency measurement between the degraded speech signal and the pre-trained reference model for each class is calculated and mapped to an objective speech quality score using data mining.Fuzzy Gaussian mixture model(GMM)is used to generate the artificial reference model trained on perceptual linear predictive(PLP)features.The mean opinion score(MOS)mapping methods including multivariate non-linear regression(MNLR),fuzzy neural network(FNN)and support vector regression(SVR)are designed and compared with the standard ITU-T P.563 method.Experimental results show that the assessment methods with data mining perform better than ITU-T P.563.Moreover,FNN and SVR are more efficient than MNLR,and FNN performs best with 14.50% increase in the correlation coefficient and 32.76% decrease in the root-mean-square MOS error.
基金National Key R&D Program of China(Grant Nos.2017YFC1702400,2018YFC1707300 and 2018YFC1707904)
文摘In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.
基金supported by the National Basic Research Program of China(2012CB932600)the National Natural Science Foundation of China(21327902,21475058,21121091)
文摘A novel disposable paper-based bipolar electrode （BPE） array is fabricated for multiplexed electrochemiluminescence （ECL） detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each consisting of six sensing cells and two reporting cells patterned using hydrophobic wax. A hairpin structure DNA assembled on the cathodes of BPEs hybridizes with Pt nanoparticles （NPs） labeled probe DNA in the presence of complementary target DNA. The introduction of Pt NPs catalyzes the reduction of dissolved 02 at cathodes and induces an enhanced ECL signal from Ru（bpy）32＋/tripropylamine （TPrA） at the anodes of BPEs. The dissolved 02 lost in reduction reaction could be promptly replenished due to the relatively large contact area of the paper-based cells with air, which ensures the stability of ECL signal. This obtained paper-based BPE array sensor showed excellent performances for the multiplexed analysis of the syphilis （Treponema pallidum） gene, the immunodeficiency virus gene （HIV） and hepatitis B virus gene （HBV）.
