[Objective] The aim was to isolate the Carbendazim-degrading bacterium, so as to provide reference for the bioremediation of carbendazim contaminated soil. [Method] A carbendazim-degrading bacterium was isolated from ...[Objective] The aim was to isolate the Carbendazim-degrading bacterium, so as to provide reference for the bioremediation of carbendazim contaminated soil. [Method] A carbendazim-degrading bacterium was isolated from a vineyard which has been applied with carbendazim for a long term; then the strain was identified using Biolog automatic analysis system and phylogenetic analysis based on 16S rDNA sequence. [Result] The strain XJ-D was identified as Rhodococcus erythropolis. It can use carbendazim as the sole carbon or nitrogen source, and degrade 99.0% of carbendazim at concentrations of 600 mg/L in mineral salt medium within 11 d. In addition, it showed a high average degradation rate of 52.87 mg/（L·d）. [Conclusion] The carbendazim-degrading bacterium XJ-D has a wide application prospect in bioremediation of pesticide-polluted soil.展开更多
[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots fro...[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots from forest section after treatment for one month were as explants,and 1 g/L carbendazim,75% ethanol,0.1% mercuric chloride and Yipeilong with different concentrations were used as disinfectants for roots disinfection.[Result] Before conventional disinfection,root explants were treated by 1 g/L carbendazim for 2.5 h,and disinfected by 75% ethanol for 30 s and 0.1% HgCl2 for 6 min,then cultured on the callus induction medium supplemented with 0.1% Yipeilong.The results showed that the contamination rate of explants decreased to 44.59%,and 25.60% explants survived after cultured for 30 d.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H.brasiliensis.展开更多
基金Supported by National Key Technology Research and Development Program of China(2007BAC20B04)~~
文摘[Objective] The aim was to isolate the Carbendazim-degrading bacterium, so as to provide reference for the bioremediation of carbendazim contaminated soil. [Method] A carbendazim-degrading bacterium was isolated from a vineyard which has been applied with carbendazim for a long term; then the strain was identified using Biolog automatic analysis system and phylogenetic analysis based on 16S rDNA sequence. [Result] The strain XJ-D was identified as Rhodococcus erythropolis. It can use carbendazim as the sole carbon or nitrogen source, and degrade 99.0% of carbendazim at concentrations of 600 mg/L in mineral salt medium within 11 d. In addition, it showed a high average degradation rate of 52.87 mg/（L·d）. [Conclusion] The carbendazim-degrading bacterium XJ-D has a wide application prospect in bioremediation of pesticide-polluted soil.
基金Supported by Natural Science Foundation of Hainan Province(30821)Basic Scientific Research Business Fees of Chinese Academy of Tropical Agricultural Sciences(XJSYWFZX2008-02)~~
文摘[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots from forest section after treatment for one month were as explants,and 1 g/L carbendazim,75% ethanol,0.1% mercuric chloride and Yipeilong with different concentrations were used as disinfectants for roots disinfection.[Result] Before conventional disinfection,root explants were treated by 1 g/L carbendazim for 2.5 h,and disinfected by 75% ethanol for 30 s and 0.1% HgCl2 for 6 min,then cultured on the callus induction medium supplemented with 0.1% Yipeilong.The results showed that the contamination rate of explants decreased to 44.59%,and 25.60% explants survived after cultured for 30 d.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H.brasiliensis.
