采用热水浸泡法提取洋葱多糖;在碱性条件下,将洋葱多糖水溶液与氯化钙反应合成洋葱多糖钙。在p H、温度、料液比、氯化钙用量四个单因素实验的基础上,采用正交试验优化洋葱多糖钙合成工艺。结果表明,最佳工艺条件为:p H 8.5,温度65℃,...采用热水浸泡法提取洋葱多糖;在碱性条件下,将洋葱多糖水溶液与氯化钙反应合成洋葱多糖钙。在p H、温度、料液比、氯化钙用量四个单因素实验的基础上,采用正交试验优化洋葱多糖钙合成工艺。结果表明,最佳工艺条件为:p H 8.5,温度65℃,料液比1∶60,氯化钙的用量2.5 m L,该条件下洋葱多糖钙的合成率为60%。多糖钙的络合物作为补钙剂对肠胃的刺激极小,无副作用,当其释放钙后,配体多糖还具有多方面的生活活性,对肌体有益,可被吸收利用。展开更多
Objective:To study the role of intracellular calcium signal pathway in the down-regulation of aggrecan induced by cyclic tensile strain in the annulus fibrosus cells. Methods:The expression of aggrecan mRNA and core p...Objective:To study the role of intracellular calcium signal pathway in the down-regulation of aggrecan induced by cyclic tensile strain in the annulus fibrosus cells. Methods:The expression of aggrecan mRNA and core protein were respectively detected with RT-PCR and western blot after the channels transmitting calcium ions were blocked with EGTA, gadolinium and verapamil. Results :EGTA. gadolinium and verapamil partially prevented the effects of cyclic tensile strain on the expression of aggrecan in annulus fibrosus cells. Conclusion:The calcium signaling is involved in the down-regulation of proteoglycan resulting from cyclic tensile strain in the annulus fibrosus cells.展开更多
文摘采用热水浸泡法提取洋葱多糖;在碱性条件下,将洋葱多糖水溶液与氯化钙反应合成洋葱多糖钙。在p H、温度、料液比、氯化钙用量四个单因素实验的基础上,采用正交试验优化洋葱多糖钙合成工艺。结果表明,最佳工艺条件为:p H 8.5,温度65℃,料液比1∶60,氯化钙的用量2.5 m L,该条件下洋葱多糖钙的合成率为60%。多糖钙的络合物作为补钙剂对肠胃的刺激极小,无副作用,当其释放钙后,配体多糖还具有多方面的生活活性,对肌体有益,可被吸收利用。
文摘Objective:To study the role of intracellular calcium signal pathway in the down-regulation of aggrecan induced by cyclic tensile strain in the annulus fibrosus cells. Methods:The expression of aggrecan mRNA and core protein were respectively detected with RT-PCR and western blot after the channels transmitting calcium ions were blocked with EGTA, gadolinium and verapamil. Results :EGTA. gadolinium and verapamil partially prevented the effects of cyclic tensile strain on the expression of aggrecan in annulus fibrosus cells. Conclusion:The calcium signaling is involved in the down-regulation of proteoglycan resulting from cyclic tensile strain in the annulus fibrosus cells.
