Cloning of Hoxc8 Promoter in Mongolian Sheep by Thermal Asymmetric Interlaced PCR

[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.

自然放牧条件下乌珠穆沁羔羊肉营养品质

乌珠穆沁羊作为我国宝贵的肉羊资源,以体格大、产肉多、肉质鲜美、适应性强等特点而著称于世。乌珠穆沁羔羊在随群放牧且没有任何补饲条件下,羔羊初生重公羔为4.07 kg、母羔3.75 kg,6月龄羔羊屠宰前平均活重35.6 kg,胴体重15.56~17.97 kg,屠宰率49.65%,净肉重13.25~14.39 kg,净肉率39.93%,其产肉量可达成年羊的45.7%。多脊椎乌珠穆沁羔羊净肉：（T14＋L7）型净肉量高于（T14＋L6）型的0.9 kg和（T13＋L7）型的0.8 kg、（T13＋L6）型的2.5 kg,净肉率分别提高1.5%、0.4%和3.0%。