Peanut (Arachis hypogaea L.) is an important source crop for edible oil and protein. It is important to identify the genetic diversity of peanut genetic resources for cultivar development and evaluation of peanut ac...Peanut (Arachis hypogaea L.) is an important source crop for edible oil and protein. It is important to identify the genetic diversity of peanut genetic resources for cultivar development and evaluation of peanut accessions. Thirty-four SSR markers were used to assess the genetic variation of four sets of twenty-four accessions each from the four botanical varieties of the cultivated peanut. Among the tested accessions, ten to sixteen pairs of SSR primers showed polymorphisms. The maximum differentiation index, which was defined as the degree of genetic differentiation, was as high as 0.992 in the tested accessions. Each accession could be discriminated by a specific set of polymorphic SSR primers, and the intra-variety genetic distance was determined among accessions, with an average of 0.59 in var. fastigiata, 0.46 in var. hypogaea, 0.38 in var. vulgaris, and 0.17 in var. hirsuta. Dendrogrames based on genetic distances were constructed for the four botanical varieties, which revealed the existence of different clusters. It was concluded that there was abundant intra-variety SSR polymorphism, and with more and more SSR markers being developed, the intrinsic genetic diversity would be detected and the development of genetic map and marker-assisted selection for cultivated peanut would be feasible.展开更多
In the study, 1 255 fungal strains were isolated from 105 peanut soil samples collected from 15 counties in 10 provinces of China. These fungi were identified to 21 genera, using the method of microscopical observatio...In the study, 1 255 fungal strains were isolated from 105 peanut soil samples collected from 15 counties in 10 provinces of China. These fungi were identified to 21 genera, using the method of microscopical observation according to morphology. And 2 genera were members of oomycetes, 3 genera of ascomycota and 16 genera of mitosporic fungi. In these soil samples, the dominant species were Penicillium, Trichoderma and Fusarium, accounting for 28.89%, 14.16% and 8.64%, respectively.展开更多
Freshwater shallow lakes typically exhibit two alternative stable states under certain nutrient loadings:macrophyte-dominated and phytoplankton-dominated water regimes.An ecosystem regime shift from macrophytes to phy...Freshwater shallow lakes typically exhibit two alternative stable states under certain nutrient loadings:macrophyte-dominated and phytoplankton-dominated water regimes.An ecosystem regime shift from macrophytes to phytoplankton blooming typically reduces the number of species of invertebrates and fishes and results in the homogenization of communities in freshwater lakes.We investigated how microbial biodiversity has responded to a shift of the ecosystem regime in Dianchi Lake,which was previously fully covered with submerged macrophytes but currently harbors both ecological states.We observed marked divergence in the diversity and community composition of bacterioplankton between the two regimes.Although species richness,estimated as the number of operational taxonomic units and phylogenetic diversity(PD),was higher in the phytoplankton dominated ecosystem after this shift,the dissimilarity of bacterioplankton community across space decreased.This decrease in beta diversity was accompanied by loss of planktonic bacteria unique to the macrophyte-dominated ecosystem.Mantel tests between bacterioplankton community distances and Euclidian distance of environmental parameters indicated that this reduced bacterial community differentiation primarily reflected the loss of environmental niches,particularly in the macrophyte regime.The loss of this small-scale heterogeneity in bacterial communities should be considered when assessing long-term biodiversity changes in response to ecosystem regime conversions in freshwater lakes.展开更多
Alfalfa (Medicago sativa L.) is being grown in harsh environment in Iraq and is mostly subjected to abiotic stresses such as drought, salinity, pH and temperature. Both alfalfa and its nitrogen fixing symbiotic bact...Alfalfa (Medicago sativa L.) is being grown in harsh environment in Iraq and is mostly subjected to abiotic stresses such as drought, salinity, pH and temperature. Both alfalfa and its nitrogen fixing symbiotic bacteria Sinorhizobium meliloti are affected by these environmental stresses. Enhancing nitrogen fixation biologically could be achieved through selection of tolerant strains of S. meliloti to these environmental stresses and inoculating them to the crop, also growing tolerant cultivars. This study examines phenotypic diversity for tolerance to drought, salinity, temperature and pH. Sixty isolates sampled from different areas of Iraq. The results revealed high degree of phenotypic diversity in Sinorhizobium populations. Furthermore, the isolates which showed tolerance to drought stress also showed tolerance to salinity and high degree of temperature, indicating direct relationship between three physiological path ways. Also 58.3% of drought tolerant isolates were alkaline tolerant they tolerated up to pH 9, point to say almost all drought tolerant isolates in this study illustrated strong + positive reaction to catalase enzyme. And 91.6% themes were negative for Gelatinase enzyme test. While only 50% of drought sensitive isolates were negative for drought sensitive isolates could grow at high temperature (42 ℃).展开更多
Inflorescences structures in context of their evolution have been conducted for 60 genera and 170 species of Celastrales (according to APG (Angiosperm Phylogeny Group) III 2009, almost 60% of genera and 15% of spec...Inflorescences structures in context of their evolution have been conducted for 60 genera and 170 species of Celastrales (according to APG (Angiosperm Phylogeny Group) III 2009, almost 60% of genera and 15% of species from this taxon of the world flora). There are two big groups of inflorescences in Celastrales-intercalary (more often) and terminal. For many genera of Celastrales both types of inflorescences can be observed, although the frequency of their occurrence varies. There is an important difference between two types of inflorescences: character of completion of the main axis (the terminal flower present or absent). Flower opening can be in basipetal (Celastraceae, Brexia) or acropetal (Stackhousiaceae) order. Partial inflorescence types included: simple, compound or umbrellate dichasia, spike, raceme, thyrse. Simple flowers of Parnassia are large, nested on long reproductive shoots, emerging from a rosette, with amplexicaule leaf.展开更多
The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyz...The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism (AFLP). 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 (0.6809-0.8648). 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus (QTLs) of Cervus nippon hortulorum.展开更多
Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To p...Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.展开更多
Aim: Ophrys insectTfera L. due to its peculiar biology and environmental requirements is among the most threatened and endangered vascular plants in Bulgaria. On the territory of Bulgaria the species fulfills the IUC...Aim: Ophrys insectTfera L. due to its peculiar biology and environmental requirements is among the most threatened and endangered vascular plants in Bulgaria. On the territory of Bulgaria the species fulfills the IUCN criteria for critically endangered [CR C2a(i); D] and is included also in the Red Data Book of Republic Bulgaria and Biodiversity Act. The present study aimed to explore and evaluate the status of its populations and their threats. Materials and methods: Exploration mad monitoring of the populations of the critically endangered orchid O. insectifera in Bulgaria was done from 2012 till 2017. The shoot count and some morphometxic parameters were recorded for evaluation of their distribution and population dynamics. Results and discussion: At one location Ophrys insectifera can be considered extinct. However two new locations were found so the total number of known locations is increased. Approximately over 50% of the occupied territories fall outside protected sites and many of the habitats axe endangered by anthropogenic or other factors. At all of the known locations there are less than 50 shoots per place. These facts put a high stress on the perspectives of their populations' survival in Bulgaria.展开更多
The Nanling and adjacent regions of South China host a series of tin deposits related to Mesozoic granites with diverse petrological characteristics. The rocks are amphibole-bearing biotite granites, or (topaz-) alb...The Nanling and adjacent regions of South China host a series of tin deposits related to Mesozoic granites with diverse petrological characteristics. The rocks are amphibole-bearing biotite granites, or (topaz-) albite-lepidolite (zinnwaldite) granites, and geochemically correspond to mealuminous and peraluminous types, respectively. Mineralogical studies demonstrate highly distinctive and critical patterns for each type of granites. In mealuminous tin granites amphibole, biotite and perthite are the typical rock-forming mineral association; titanite and magnetite are typical accessory minerals, indicating highjO2 magmatic conditions; cassiterite, biotite and titanite are the principal Sn-bearing minerals; and pure cassiterite has low trace-element contents. However, in peraluminous tin granites zirmwaldite-lepidolite, K-feldspar and albite are typical rock-forming minerals; topaz is a common accessory phase, indicative of high peraluminity of this type of granites; cassiterite is present as a uniquely important tin mineral, typically rich in Nb and Ta. Mineralogical distinction between the two types of tin granites is largely controlled by redox state, volatile content and differentiation of magmatic melts. In oxidized metaluminous granitic melts, Sn4+ is readily concentrated in Ti-bearing rock-forming and accessory minerals. Such Sn-bearing minerals are typical of oxidized tin granites, and are enriched in granites at the late fractionation stage. In relatively reduced peraluminous granitic melts, Sn2+ is not readily incorporated into rock-forming and accessory minerals, except for cassiterite at fractionation stage of granite magma, which serves as an indicator of tin mineralization associated with this type of granites. The nature of magma and the geochemical behavior of tin in the two types of granites thus result in the formation of different types of tin deposits. Metaluminous granites host disseminated tin mineralization, and are locally related to deposits of the chlorite quartz-vein, greisen, and skarn types. Greisen, skarn, and quartz-vein tin deposits can occur related to peraluminous granites, but disseminated mineralization of cassiterite is more typical.展开更多
To simulate a multivariate density with multi_hump, Markov chainMonte Carlo method encounters the obstacle of escaping from one hump to another, since it usually takes extraordinately long time and then becomes practi...To simulate a multivariate density with multi_hump, Markov chainMonte Carlo method encounters the obstacle of escaping from one hump to another, since it usually takes extraordinately long time and then becomes practically impossible to perform. To overcome these difficulties, a reversible scheme to generate a Markov chain, in terms of which the simulated density may be successful in rather general cases of practically avoiding being trapped in local humps, was suggested.展开更多
基金This work was supported by Guangxi Natural Sciences Foundation (No. 0542027) Science and Technology Development Foundation of GXAAS (No. 200301).
文摘Peanut (Arachis hypogaea L.) is an important source crop for edible oil and protein. It is important to identify the genetic diversity of peanut genetic resources for cultivar development and evaluation of peanut accessions. Thirty-four SSR markers were used to assess the genetic variation of four sets of twenty-four accessions each from the four botanical varieties of the cultivated peanut. Among the tested accessions, ten to sixteen pairs of SSR primers showed polymorphisms. The maximum differentiation index, which was defined as the degree of genetic differentiation, was as high as 0.992 in the tested accessions. Each accession could be discriminated by a specific set of polymorphic SSR primers, and the intra-variety genetic distance was determined among accessions, with an average of 0.59 in var. fastigiata, 0.46 in var. hypogaea, 0.38 in var. vulgaris, and 0.17 in var. hirsuta. Dendrogrames based on genetic distances were constructed for the four botanical varieties, which revealed the existence of different clusters. It was concluded that there was abundant intra-variety SSR polymorphism, and with more and more SSR markers being developed, the intrinsic genetic diversity would be detected and the development of genetic map and marker-assisted selection for cultivated peanut would be feasible.
基金Supported by the Major Projects for National Agricultural Products Quality Safety Risk Assessment of China(GJFP2017001004)the Fund of the Key Laboratory for Biotoxin Detection of the Ministry of Agriculture of China~~
文摘In the study, 1 255 fungal strains were isolated from 105 peanut soil samples collected from 15 counties in 10 provinces of China. These fungi were identified to 21 genera, using the method of microscopical observation according to morphology. And 2 genera were members of oomycetes, 3 genera of ascomycota and 16 genera of mitosporic fungi. In these soil samples, the dominant species were Penicillium, Trichoderma and Fusarium, accounting for 28.89%, 14.16% and 8.64%, respectively.
基金Supported by the National Natural Science Foundation of China(Nos.U1202231,31225004)the National Science Foundation for Young Scientists of China(No.31200383)
文摘Freshwater shallow lakes typically exhibit two alternative stable states under certain nutrient loadings:macrophyte-dominated and phytoplankton-dominated water regimes.An ecosystem regime shift from macrophytes to phytoplankton blooming typically reduces the number of species of invertebrates and fishes and results in the homogenization of communities in freshwater lakes.We investigated how microbial biodiversity has responded to a shift of the ecosystem regime in Dianchi Lake,which was previously fully covered with submerged macrophytes but currently harbors both ecological states.We observed marked divergence in the diversity and community composition of bacterioplankton between the two regimes.Although species richness,estimated as the number of operational taxonomic units and phylogenetic diversity(PD),was higher in the phytoplankton dominated ecosystem after this shift,the dissimilarity of bacterioplankton community across space decreased.This decrease in beta diversity was accompanied by loss of planktonic bacteria unique to the macrophyte-dominated ecosystem.Mantel tests between bacterioplankton community distances and Euclidian distance of environmental parameters indicated that this reduced bacterial community differentiation primarily reflected the loss of environmental niches,particularly in the macrophyte regime.The loss of this small-scale heterogeneity in bacterial communities should be considered when assessing long-term biodiversity changes in response to ecosystem regime conversions in freshwater lakes.
文摘Alfalfa (Medicago sativa L.) is being grown in harsh environment in Iraq and is mostly subjected to abiotic stresses such as drought, salinity, pH and temperature. Both alfalfa and its nitrogen fixing symbiotic bacteria Sinorhizobium meliloti are affected by these environmental stresses. Enhancing nitrogen fixation biologically could be achieved through selection of tolerant strains of S. meliloti to these environmental stresses and inoculating them to the crop, also growing tolerant cultivars. This study examines phenotypic diversity for tolerance to drought, salinity, temperature and pH. Sixty isolates sampled from different areas of Iraq. The results revealed high degree of phenotypic diversity in Sinorhizobium populations. Furthermore, the isolates which showed tolerance to drought stress also showed tolerance to salinity and high degree of temperature, indicating direct relationship between three physiological path ways. Also 58.3% of drought tolerant isolates were alkaline tolerant they tolerated up to pH 9, point to say almost all drought tolerant isolates in this study illustrated strong + positive reaction to catalase enzyme. And 91.6% themes were negative for Gelatinase enzyme test. While only 50% of drought sensitive isolates were negative for drought sensitive isolates could grow at high temperature (42 ℃).
文摘Inflorescences structures in context of their evolution have been conducted for 60 genera and 170 species of Celastrales (according to APG (Angiosperm Phylogeny Group) III 2009, almost 60% of genera and 15% of species from this taxon of the world flora). There are two big groups of inflorescences in Celastrales-intercalary (more often) and terminal. For many genera of Celastrales both types of inflorescences can be observed, although the frequency of their occurrence varies. There is an important difference between two types of inflorescences: character of completion of the main axis (the terminal flower present or absent). Flower opening can be in basipetal (Celastraceae, Brexia) or acropetal (Stackhousiaceae) order. Partial inflorescence types included: simple, compound or umbrellate dichasia, spike, raceme, thyrse. Simple flowers of Parnassia are large, nested on long reproductive shoots, emerging from a rosette, with amplexicaule leaf.
文摘The genetic diversity of 27 different Cervus nippon hortulorum was studied to provide theoretical evidence for their identification and utilization. The genomie DNA of 27 different Cereus nippon hortulorum were analyzed by amplified fragment length polymorphism (AFLP). 11,443 bands associated with genetic polymorphism among total 15,169 bands were obtained with 9 kinds of primer pairs and restriction endonuclease EcoRI/Msel, percentage of polymorphie band was 78.43%, 1,271 polymorphic locus were shown per primer pair. The AFLP data showed that average genetic similarity was 0.7841 (0.6809-0.8648). 27 samples were classified into Group I and Group II with cluster analysis, and Group II was divided into five subgroups. The result of AFLP and cluster analysis concluded that there was high genetic variation, which associated with orientated artificial breed selection and breeding in the population. Genetic similarity of Group II-4 was the highest, more than 0.82, while genetic distance in this group was the shortest, from 0.1354 to 0.1563, which was coordinated with breeding record. All these showed that there was great genetic polymorphism among the deer population. The results laid the foundation for main quantitative trait locus (QTLs) of Cervus nippon hortulorum.
基金supported by the National Natural Science Foundation of China(30870308 and 31071944)the Shanghai Rising-Star Program(10QA1402200)
文摘Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.
文摘Aim: Ophrys insectTfera L. due to its peculiar biology and environmental requirements is among the most threatened and endangered vascular plants in Bulgaria. On the territory of Bulgaria the species fulfills the IUCN criteria for critically endangered [CR C2a(i); D] and is included also in the Red Data Book of Republic Bulgaria and Biodiversity Act. The present study aimed to explore and evaluate the status of its populations and their threats. Materials and methods: Exploration mad monitoring of the populations of the critically endangered orchid O. insectifera in Bulgaria was done from 2012 till 2017. The shoot count and some morphometxic parameters were recorded for evaluation of their distribution and population dynamics. Results and discussion: At one location Ophrys insectifera can be considered extinct. However two new locations were found so the total number of known locations is increased. Approximately over 50% of the occupied territories fall outside protected sites and many of the habitats axe endangered by anthropogenic or other factors. At all of the known locations there are less than 50 shoots per place. These facts put a high stress on the perspectives of their populations' survival in Bulgaria.
基金supported by the National Natural Science Foundation of China(Grant No.41230315)the National Key R&D Program of China(Grant No.2016YFC0600203)the Fundamental Research Funds for the Central Universities(Grant No.020614380057).
文摘The Nanling and adjacent regions of South China host a series of tin deposits related to Mesozoic granites with diverse petrological characteristics. The rocks are amphibole-bearing biotite granites, or (topaz-) albite-lepidolite (zinnwaldite) granites, and geochemically correspond to mealuminous and peraluminous types, respectively. Mineralogical studies demonstrate highly distinctive and critical patterns for each type of granites. In mealuminous tin granites amphibole, biotite and perthite are the typical rock-forming mineral association; titanite and magnetite are typical accessory minerals, indicating highjO2 magmatic conditions; cassiterite, biotite and titanite are the principal Sn-bearing minerals; and pure cassiterite has low trace-element contents. However, in peraluminous tin granites zirmwaldite-lepidolite, K-feldspar and albite are typical rock-forming minerals; topaz is a common accessory phase, indicative of high peraluminity of this type of granites; cassiterite is present as a uniquely important tin mineral, typically rich in Nb and Ta. Mineralogical distinction between the two types of tin granites is largely controlled by redox state, volatile content and differentiation of magmatic melts. In oxidized metaluminous granitic melts, Sn4+ is readily concentrated in Ti-bearing rock-forming and accessory minerals. Such Sn-bearing minerals are typical of oxidized tin granites, and are enriched in granites at the late fractionation stage. In relatively reduced peraluminous granitic melts, Sn2+ is not readily incorporated into rock-forming and accessory minerals, except for cassiterite at fractionation stage of granite magma, which serves as an indicator of tin mineralization associated with this type of granites. The nature of magma and the geochemical behavior of tin in the two types of granites thus result in the formation of different types of tin deposits. Metaluminous granites host disseminated tin mineralization, and are locally related to deposits of the chlorite quartz-vein, greisen, and skarn types. Greisen, skarn, and quartz-vein tin deposits can occur related to peraluminous granites, but disseminated mineralization of cassiterite is more typical.
文摘To simulate a multivariate density with multi_hump, Markov chainMonte Carlo method encounters the obstacle of escaping from one hump to another, since it usually takes extraordinately long time and then becomes practically impossible to perform. To overcome these difficulties, a reversible scheme to generate a Markov chain, in terms of which the simulated density may be successful in rather general cases of practically avoiding being trapped in local humps, was suggested.