目的探讨多被银莲花总皂甙对人涎腺腺样囊性癌高肺转移细胞(ACC-M)内Bcl-2和Bax蛋白表达的影响。方法将体外培养的ACC-M细胞随机分为实验组与对照组,分别加入50μg/m L多被银莲花总皂甙溶液2 m L、含同样浓度二甲基亚砜的RPMI1640培养液...目的探讨多被银莲花总皂甙对人涎腺腺样囊性癌高肺转移细胞(ACC-M)内Bcl-2和Bax蛋白表达的影响。方法将体外培养的ACC-M细胞随机分为实验组与对照组,分别加入50μg/m L多被银莲花总皂甙溶液2 m L、含同样浓度二甲基亚砜的RPMI1640培养液2 m L。分别于培养24、48、72 h用Western blotting法检测细胞内Bcl-2和Bax蛋白的表达量。结果对照组各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均无统计学意义(P均>0.05);实验组各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均有统计学意义(P均<0.05);两组间各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均有统计学意义(P均<0.05)。结论多被银莲花总皂甙可上调ACC-M细胞内Bax蛋白表达,同时下调Bcl-2蛋白表达,导致Bax/Bcl-2升高。展开更多
Chinese folk medicine “Liang Tou Jian”(Anemone raddeana Regel) is the important medicine used to treat rheumatism, neuralgia, cancer and so on. Its chemical constituents were studied by chromatographing on silica ge...Chinese folk medicine “Liang Tou Jian”(Anemone raddeana Regel) is the important medicine used to treat rheumatism, neuralgia, cancer and so on. Its chemical constituents were studied by chromatographing on silica gel and HPLC. A new triterpenoid saponin, raddeanoside 18, along with two known triterpenoid saponins, Hederacholichiside F and Leontoside D, was isolated from the ethanolic extrant of the rhizoma of Anemone raddeana Regel. Their structures were elucidated as 3-O-β-D-glucopyranosyl(1→4)[β-D-glucopyranosyl(1→2)]-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(1), 3-O-β-D-glucopyranosyl(1→4)[α-L-rhamnopyranosyl(1→2)-]-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(2), 3-O-β-D-glucopyranosyl(1→4)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(3) by 1D and 2D NMR ESI-MS and IR spectrometries, respectively.展开更多
文摘目的探讨多被银莲花总皂甙对人涎腺腺样囊性癌高肺转移细胞(ACC-M)内Bcl-2和Bax蛋白表达的影响。方法将体外培养的ACC-M细胞随机分为实验组与对照组,分别加入50μg/m L多被银莲花总皂甙溶液2 m L、含同样浓度二甲基亚砜的RPMI1640培养液2 m L。分别于培养24、48、72 h用Western blotting法检测细胞内Bcl-2和Bax蛋白的表达量。结果对照组各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均无统计学意义(P均>0.05);实验组各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均有统计学意义(P均<0.05);两组间各时间点Bcl-2、Bax蛋白相对表达量及Bax/Bcl-2比较差异均有统计学意义(P均<0.05)。结论多被银莲花总皂甙可上调ACC-M细胞内Bax蛋白表达,同时下调Bcl-2蛋白表达,导致Bax/Bcl-2升高。
文摘Chinese folk medicine “Liang Tou Jian”(Anemone raddeana Regel) is the important medicine used to treat rheumatism, neuralgia, cancer and so on. Its chemical constituents were studied by chromatographing on silica gel and HPLC. A new triterpenoid saponin, raddeanoside 18, along with two known triterpenoid saponins, Hederacholichiside F and Leontoside D, was isolated from the ethanolic extrant of the rhizoma of Anemone raddeana Regel. Their structures were elucidated as 3-O-β-D-glucopyranosyl(1→4)[β-D-glucopyranosyl(1→2)]-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(1), 3-O-β-D-glucopyranosyl(1→4)[α-L-rhamnopyranosyl(1→2)-]-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(2), 3-O-β-D-glucopyranosyl(1→4)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(3) by 1D and 2D NMR ESI-MS and IR spectrometries, respectively.
